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Hymenoscyphus repandus
Steve Clements, 21-07-2015 15:32
Bonjour,
Trouvé le 15 Juillet sur fleur morte. Je crois qu'il est Hymenoscyphus repandus, mais il ya beaucoup de questions au sujet de cette espèce sur Asco France. C'est pourquoi je n'en suis pas sûr. Une confirmation serait très appréciée.
Cordialement,
Steve

Hi,
Found on July 15 on dead flower remains in mixed woodland. I work this out to be Hymenoscyphus repandus, but I note that there are many queries about this species on Asco France. A confirmation would be very much appreciated.
"Cap" up to 1.7mm, "stem" up to 2mm (0.3mm wide at apex, 0.2mm wide at base, which is a little tormentose). Colour is dull yellow all over. Shed spores 11-13.5 x 2.5-3, measured in water. Asci 65-75 x 6, blueing in Lugol. Spores arranged in a roughly biseriate fashion. Paraphyses threadlike, 2.5 wide.
I was perplexed by the sharply pointed, refractive hair-like structures in my second picture. Are these part of the fungus or accidental inclusions?
Kind regards,
Steve
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Hans-Otto Baral, 21-07-2015 17:08
Hans-Otto Baral
Re : Hymenoscyphus repandus
Hi Steve

this looks quite correct. It seems like being part of the plant between the apothecia.

Are you sure with the absence of croziers? Because the species may sometimes occur with croziers. To me it looks like without, but this could be better seen.

Only some of the spores are alive in your preparations. In living asci the spores would be larger and more accurately obliquely biseriate.

By accident I got today a sequence of H. repandus, and it falls near H. menthae as I expected. The two are rather marginal within Hymenoscyphus.

Zotto
Steve Clements, 21-07-2015 20:09
Re : Hymenoscyphus repandus
Thanks very much Zotto,
The croziers aren't very pronounced.
It's a beautiful little asco! 
Kind regards,
Steve
Hans-Otto Baral, 21-07-2015 21:53
Hans-Otto Baral
Re : Hymenoscyphus repandus
The feature of the crozier is quite a consistent and unequivocal character. I presume that diferent species are involved when H. repandus appears with croziers, which is the rarer case.

It helps to stain with Congo Red to see them better. I think your sample is without.

Zotto
Steve Clements, 21-07-2015 23:26
Re : Hymenoscyphus repandus
Hello Zotto,
I have checked for croziers using Congo Red and oil immersion. Interestingly I could find only one ascus which looks as though it has something like a crozier. All the rest I looked at do not have croziers.
Steve
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Hans-Otto Baral, 22-07-2015 06:46
Hans-Otto Baral
Re : Hymenoscyphus repandus
Thanks! I think they are clearly without, could not find a case looking like a crozier.

Did you sample the species in England, and have you any idea about the plant?

Zotto
Steve Clements, 22-07-2015 19:13
Re : Hymenoscyphus repandus
Hi Zotto,
The specimen was from the Longshaw Estate in the Peak District National Park, Derbyshire, N.England.
I don't know the species of the host.
Steve
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Hans-Otto Baral, 22-07-2015 20:14
Hans-Otto Baral
Re : Hymenoscyphus repandus
Thanks! Surely an Asteraceae (Composite). Reminds me of an artichoke.
Steve Clements, 24-07-2015 12:43
Re : Hymenoscyphus repandus
Hi Zotto,
We used the GPS to find the exact location - the only compositae present is Cirsium arvense - Creeping Thistle. We found more old flower remains on the ground, but were unable to find any more of the fungus,
Regards,
Steve
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Hans-Otto Baral, 24-07-2015 17:12
Hans-Otto Baral
Re : Hymenoscyphus repandus
Great, this sounds reasonable!