Dear Viktorie

Great, I cannot remember such a fungus. Did you study the anamorph also? How sure does this belong to the apothecia in your opinion?

Zotto

Dear Zotto,

thank you very much. I feared I'll get answer like this since I didn't find anything similar in your folder. : ) 

I didn't study it yet in detail, I'll try to do that today evening and test the KOH as well. But here's the upper part of one conidioma (pyknid? don't know correct term) where I measured the conidia and it has the same gel layer. I don't know how common this feature is among inoperculates. 

I wanted to test it with CRB, but still have some problems with this reagent - either it's too concentrated (and staind everything violet) or I get no reaction at all. Could you please advise me the correct way to apply it? I tried it with the medium becoming only light blue, which avoided the overstaining, but never got those beautiful turquise VBs (in other species).

Viktorie

I simply add CRB to the waer mount and let it diffuse. The size of the dopr determines the concentration. Of course, CRB must be aqueous, not dissolved in another reagent.

Not sure what you want, maybe staining of the gel layer? That should work, usually.

Bonjour, 

Nous pensons avoir trouvé la même espèce que celle présentée par Viktorie dans ce forum :

Trouvé sur les branches de Prunus padus, apothécies de 0,5 - 1 mm de diamètre, isolé à grégaire, hyménium gris.

Ascospores : 8,5 - 11 x 2,25 - 2,75 µm, hyalines, conidies 5,75 - 7,5 x 1,5 - 2,25µm, asques 52 - 70 x 7 - 8 µm, avec crochets, IKI+, paraphyses légèrement élargies à l'extrémité.

Merci pour vos commentaires.

Elisabeth

Dear Elisabeth, 

great, it's indeed the same fungus. Would you please send me the collection data? Meanwhile I identified it as Glutinium laevatum, a species described several times in anamorph stage.
edit: was it on Padus avium, or P. serotina? 

If you ever find something similar on Salix (maybe shrub Salix?), we would be much interested in studying and sequencing it. (There's a sister species, perhaps less common, G. palinum. I have Petrak's locality nearby, but haven't found it there yet.) 

Viktorie

Magnifique, merci Viktorie.

Je t'envoie les données de la récolten par courriel.

Elisabeth