micro dessin

Poils excipulum...

Ce sera plus explicite sur ce dessin...

Hi Yannick

looks for a species around Hymenoscyphus fuscidulus. I would term the ascus apex mammiform. Very important would be the content of the living paraphyses, I assume they contain strongly refractive vacuoles. Also I doubt that they are normally pointed. Could you please verify this?

The ectale excipulum seems of globulosa. It would be good to have a picture of unstained cells with their natural pigment.

Also the drawn spores are dead, do you have the fungus only in the dry state?
Cheers
Zotto

Thank's Zotto.
Yes, unfortunately my fungus is dry now.
I looked for refractive vacuoles in paraphyses when it wasn't dry, but I saw nothing. Is-it possible to see them when dry ? May be all collapsed.
I try to make a picture of ectale excipulum now.
Yannick

These fungi are entirely xerointolerant, therefore these VBs totally disappear.

What you can try is a photo of the IKI-stained ascus apex. The H. fulvidulus group has a speciel rather conspicuous apical ring, unlike typical Hymenoscyphus.

Zotto

Zotto,

I have found some paraphyses with oil content. Their apex is rounded.
Here is too a photo of the ectal excipulum.
I wasn't able to take a photo of the IKI ascus reaction : problem with my camera.

Yannick

paraphyses

O.k., the drops in the paraphyses are possibly oil. The tips are indeed a bit tapered. If the spores were not that large I would even suggest a Mollisia. But also the oil in the paraphyses seems unusual for a Mollisia.

Zotto

Thanks Zotto. I put it on the rubbish-bin ?

I think this is a very interesting collection, perhaps a rare species. A pitty that we do not have its vital characters.

Zotto

Do you want me to send it to you ?
Yannick

If you wish you can do. I can at least try what I can.

Zotto

Now we come to a better result. I received today the wonderful specimen though in the dry state (only part of the spores were alive. It is clearly a Mollisia (!, e.g., because of the Calycina-type of apical ring, the amyloid zones being apicallywidest), and very close to M. ramealis (= Dibeloniella citrinella). I did not come to a result with Andreas' english key. The only difference to M. ramealis seems to me to be in spore spize, being much too short, and a bit too wide.

My data: Asci KOH 98-107 x 8.3-9 µm, with croziers, IKI 3rb (distinctly red!), Calycina-type. Sp. *19-21.5 x 3.7-4 µm. Paraph. KOH 3-3.8 µm wide, not or slightly obtusely lanceolate. Hymenium KOH-. Medulla very loose t. intricata, with a few some crystal druses.

The large drops in the spores are obviously a product of drying since I found some spores with a purely multiguttulate content. They then resemble those of Hymenoscyphus menthae.

So please tell me the collection data. Is the bark actually Betula? It does not at all look white. You found it on a hanging, still-attached branch?

Zotto

The conical, slightly mammiform ascus apex is also untypical for Hymenoscyphus s.l.

Excipulum only on lower flanks of vertically oriented t. globulosa, on upper flanks running under 30-40°, of. short-celled t. prismatica. The same is true in M. ramealis, also these hairs are typical there.

Thank's a lot Zotto.

I found this Mollisia on a died branch, still in place, in 2m from height, on Betula.
All the bark of this Betula was not completely white, but I am sure that it was a Betula.

The collection is from "Forêt Communale de Pelouse", Pelouse (48000-Lozère-France), 26/08/2009.

Is-it possible to have these tree pictures in highter resolution ?

Yannick

Thanks, Yannick,

yes, I send you my images by email

Zotto