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Bonjour, Quelqu'un peut il me donner un conseil p

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Mathias Hass Mathias Hass

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Åge Oterhals

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Thomas Læssøe

https://svampe.databasen.org/observations/10584971

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Ascobolus michaudii with possible bycatch Pyxidiophora arvernensis?
François Bartholomeeusen, 18-05-2025 16:49
Dear forum members,

On cow dung, between fruiting bodies of Lasiobolus papillatus, I found almost cylindrical ascomata with a diameter < 2 mm. The disc is yellowish with dentate margin.

Spores: elliptical with gelatinous coating, with longitudinal anastomosing lines, from hyaline to purple; (18.6) 20 - 24.3 (24.8) × (10.4) 11 - 11.9 (12.3) µm; Q = (1.6) 1.8 - 2.1 (2.2) ; Me = 22.3 × 11.4 µm ; Qe = 2
Asci: elongated club-shaped, Me = 210.5 × 27.4 µm ; Qe = 7.7; slight purple discolouration in Melzer:
Paraphysis: filiform, with multiple septa, width about 3.5 µm in a yellowish substance. After some days in moist environment I observe fortoulisme.
Ectal excipulum: textura globulosa.

Could this be Ascobolus michaudii?

Thanks in advance,

François Bartholomeeusen

N.B.: on two photos during microscopic examination I found clustered spores with the following dimensions: (52.3) 52.33 - 58 × (4.4) 4.42 - 5.15 (5.2) µm: Me = 55 × 4.9 µm ; Qe = 11.6.
I found only these spores and no perithecial beak. Could these spores belong to Pyxidiophora arvernensis (Laboulbeniomycete)?

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Peter Welt, 19-05-2025 10:55
Peter Welt
Re : Ascobolus michaudii with possible bycatch Pyxidiophora arvernensis?
Certainly a species of the genus Pyxidiophora. However, not P. arvernensis, since the asci have eight spores. Probably P. spinuliformis (Speg., 1909) N. Lundq., since all other species have fewer than eight spores.
See: Doveri, F. & B. Coue (2006) - First record of Pyxidiophora badiorostris from France. Doc. Myco
l. 34/ 133- 134: 33-41.

The article is available online.

Best regards, Peter
François Bartholomeeusen, 19-05-2025 15:06
Re : Ascobolus michaudii with possible bycatch Pyxidiophora arvernensis?
Dear Peter,

Thank you very much for your response. I think you are right. I also used the key in that publication, but I was unsure about the number of spores and their length. I suspect the spores are not fully mature.
Thanks again, now I only need to obtain confirmation about Ascobolus michaudii!

Many greetings,
François