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Tiny asco on Fallopia
Camille Mertens,
03-04-2023 12:39
Collected on Fallopia end of january.
Spores slighty curved, guttulated, wrapped in a sheet : 11-12 x 3,6-3,9 µm
Ascus with croziers, IKI (-)
Branched paraphysis.
Seems to me closed to Allophylaria "petasites" on Zotto's webside illutrations.
An idea?
Best regards.
Camille
Allophylaria-sp.-bis-0001.pdf
Hans-Otto Baral,
03-04-2023 14:44
Re : Tiny asco on Fallopia
Hi Camille
that is indeed similar, although Björn measured (9-)10,3-12,4x2,6-3µm.
Your IKI I do not much trust, or you need to remove the KOH better, or even better let KOH away and show what IKI does.
The marginal excipulum doe snot look like an Allophylaria, in Björn's specimen the cells are elongate and thick-walled (gel). Did you also see VBs in the paraphyses that get red-brown in IKI by extruding red granules?
Zotto
Camille Mertens,
03-04-2023 19:43
Re : Tiny asco on Fallopia
Hi Hans
I agree this does not fit an Allophylaria.
IKI was negative. Therefore I tried KOH pretreatment.
I cannot remember a red-brown reaction in IKI.
I add a photo of paraphysis in Crb.
Many thanks.
Camille
I agree this does not fit an Allophylaria.
IKI was negative. Therefore I tried KOH pretreatment.
I cannot remember a red-brown reaction in IKI.
I add a photo of paraphysis in Crb.
Many thanks.
Camille
Hans-Otto Baral,
03-04-2023 20:25
Re : Tiny asco on Fallopia
Sorry, these paraphyses are dead, there I cannot see anything. They must be alive, best in water. Not sure how to proceed. The excipulum near the base could be helpful too.
I see a minute reactive (amyloid) ring in your ascus (KOH+IKI). But since the photo does not show any iodine colour (yellow-brown), I wonder how this would turn at higher IKI concentration.
Camille Mertens,
03-04-2023 20:39
Re : Tiny asco on Fallopia
Many thanks Hans.
Gonna try to collect new ones.
Gonna try to collect new ones.