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22-05-2024 23:39

Marc Detollenaere Marc Detollenaere

Dear Forum,On debarked Fagus I found some small wh

21-05-2024 17:48

Karl Soler Kinnerbäck

Hi all,Could this be Venturioscypha or Venturiocis

21-05-2024 11:33

Nihad Omerovic

Hello,found on dead, dry, attached (and fallen) tw

07-11-2018 08:34

Zuzana Sochorová (Egertová) Zuzana Sochorová (Egertová)

Hello, could someone send this publication to me

21-05-2024 09:15

Pierre Repellin

Bonjour à tous,L'ouvrage de Björn Wergen: Handbo

21-05-2024 16:12

Thomas Flammer

Substrate: not quite sure, on pluvious stem of a h

21-05-2024 11:13

Ursula Kozik

Hallo und guten Tag, habe eine Frage zur Farbe ei

17-05-2024 16:25

Pavel Jiracek

Erioscyphella lunata, found on a fallen needle of

20-05-2024 21:28

Nihad Omerovic

Hello,Found this Orbilia on a dry, attached twig o

18-05-2024 10:47

Anna Klos

Goodmorning,We found this tiny ascomycete (max. 1m

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Whitish inoperculate on the liverwort Aneura pinguis
Edvin Johannesen, 25-03-2023 14:42
Edvin JohannesenI have received this dried specimen for microscopy. In the fresh state the apothecia are (purplish-greyish) white, but they become black in the dry state (see photos of both states). On rehydration they appear somewhat gelatinous, dark purplish brown.
Hymenium is a brown, dense palisade. Excipulum is paler brown, I would say textura epidermoidea (?). Asci and spores appear dead. Mature spores are brown, 20 x 6 micr., ellipsoid/allantoid, aseptate or I suspect becoming 1-septate (hard to see).  Asci IKI negative. 
All micro images in water.
I have not been able to find any bryophilous inoperculates with such brown spores.

Any suggestions?
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Georges Greiff, 29-03-2023 13:03
Re : Whitish inoperculate on the liverwort Aneura pinguis
Very interesting collection. I have not seen any inoperculates on liverworts with brown spores. It may well be undescribed. Bryoscyphus is superficially similar and seeing living paraphyses would have helped. Bryoscyphus conocephali agg. is on liverworts in similar habitats. It is not that but it might be related.

Edvin Johannesen, 29-03-2023 14:55
Edvin Johannesen
Re : Whitish inoperculate on the liverwort Aneura pinguis
Thanks for your opinion, Georges.  I will have another attempt at looking for paraphyses (I doubt they will be alive). I assume that the spore color is not a result of necrosis or some other process?
Georges Greiff, 29-03-2023 15:19
Re : Whitish inoperculate on the liverwort Aneura pinguis
Dear Edvin,

I have not noted brownish spores in any of the herbarium material of Bryoscyphus that I have examined, but that doesn't mean it won't happen. The darkening of the dry, old apothecia is a common occurrence Bryoscyphus, including in B. conocephali I collected recently. Perhaps this pigment has gone into the spores. I can check if this darkening happens in spores of my B. conocephali.

Unfortunately the paraphyses will be dead so you won't see the VBs. Perhaps now only sequencing can place this collection with more certainty within one of the bryophilous Leotiomycete groups. They are tricky even when fresh.

All the best,
Edvin Johannesen, 29-03-2023 15:40
Edvin Johannesen
Re : Whitish inoperculate on the liverwort Aneura pinguis
It would be great if you could check this - thanks!