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Hello, bonjour.Here is the paper I'm searching for
Lachnum subvirgineum with blue/purple hymenium?
François Bartholomeeusen,
11-07-2022 18:41
At the dried out edge of a swamp, on plant debris, I found dozens of ascomata of Lachnum (diameter < 2 mm). On twigs (alder, willow, birch, oak) with diameters < 10 mm and also on an alder catkin.
The hymenium is white and turns brown after damaging or ageing. Very striking: some ascomata have a bright blue/purple hymenium (in the field I remember more blue!).
Miccroscopy:
a) Hairs without oxalate crystals, almost no refractive vacuoles and apex capitate, 44-70 x 3-5 µm
b) Paraphyses only very faint refractive vacuoles; 53-57 x 3.7-5.3 µm; protruding 14-17 µm
c) Asci with croziers, IKI +; 46-59 x 3.7-5 µm
d) Spores: (6.2) 6.4 - 8.1 (9.9) × (1.1) 1.6 - 2.1 (2.3) µm; Q = 3.2 - 4.6; Me = 7.3 × 1.9 µm ; Qe = 3.9
On microscopic examination of the blue/purple-coloured ascomata, I had the impression that they were overripe. Spores were floating massively in a pulp of conidia in which a slight shade of blue was visible. In another specimen, very fine hyphae turned blue when the hymenium was cut with a scalpel.
Is Lachnum subvirgineum a correct determination? What can be the cause of the blue/purple discolouration, does anyone have experience with this phenomenon?
Thank you very much in advance,
François Bartholomeeusen
Hans-Otto Baral,
11-07-2022 20:31

Re : Lachnum subvirgineum with blue/purple hymenium?
Lachnum subvirgineum is good. The globose drop in the paraphysis is an SCB, similar SCBs may occur in the hairs, also in L. virgineum.
I know this this blue-violet stain. In your last photo it is probable that numerous bacteria are the origin.
I do not remember in which Lachnum species I saw this, but there I was sure that bacteria cause the stain in decaying apos.
François Bartholomeeusen,
11-07-2022 20:38
Re : Lachnum subvirgineum with blue/purple hymenium?
Dear Zotto,
Mystery solved! Thank you very much for your quick response.
Greetings,
François