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This tiny, rather "rough" erumpent asco was found
Lachnum on Rubus.
Mirek Gryc,
10-07-2022 16:57
Unfortunately, I have a problem with the interpretation of some features and especially with the contents of paraphyse. Watching the preprat from the ascomata just after the harvest, I saw yellowish content. After two days of breeding, the hymenium became almost white and with this also the paraphyse content.
The key leads me to L. winteri?
Spores - the first ascomata;
(9.4) 9.6 - 10.9 (12.2) × (2.3) 2.4 - 2.7 µm
Q = (3.6) 3.8 - 4.6 (5.2); N = 8
Me = 10.4 × 2.5 µm; QE = 4.2
Spores - the second ascomata;
(8.8) 9.3 - 10.8 (11.3) × (2) 2.2 - 2.5 (2.6) µm
Q = (3.8) 4 - 4.7 (4.8); N = 15
Me = 10 × 2.3 µm; QE = 4.3
Most hair with a length of about 60 µm the longest up to 80µm.
Stipe about 0.2mm.
Paraphyse protruding above hymenium up to 28 µm.
Regards
Mirek
Hans-Otto Baral,
10-07-2022 17:20
Re : Lachnum on Rubus.
Hmm, difficult to say when there are not enough living hairs and paraphyses in water. Hairs in CRB look like containing VBs but I am not sure.
Lachnum clavigerum seems to be similar but has crystals. Other options I cannot see. Lachnum winteri I only know on Phragmites and often has yellow colour (VBs).
Mirek Gryc,
10-07-2022 18:19
Re : Lachnum on Rubus.
Hi Zotto
In the water, the content is almost invisible so that's why I showed more photos with a dyed preparation. This remark applies to both paraphyses and hair.
There were certainly no crystals on the hair.
In one of the fruiting bodies I saw crystals but only in excipulum. However, this was not a permanent feature, so I did not mention it.
I don't know L. clavigerum, but as I can see, he has much smaller spores?
Thank you Zotto.
Mirek
In the water, the content is almost invisible so that's why I showed more photos with a dyed preparation. This remark applies to both paraphyses and hair.
There were certainly no crystals on the hair.
In one of the fruiting bodies I saw crystals but only in excipulum. However, this was not a permanent feature, so I did not mention it.
I don't know L. clavigerum, but as I can see, he has much smaller spores?
Thank you Zotto.
Mirek
Hans-Otto Baral,
10-07-2022 21:47
Re : Lachnum on Rubus.
In my folders L. clavigerum has equally large spores, depending on the substrate:
clavigerum Rosaceae (8-)9-11(-12) x 2-2.5
clavigerum Epilobium, Aruncus 6-9 x 1.7-2
Mirek Gryc,
11-07-2022 09:02
Re : Lachnum on Rubus.
Zotto, you are probably right and I am even convinced that you are definitely right.
Something is wrong with these fruiting bodies! They microscopic very hard because their condition is very weak, so I can't get a clear picture. There is no wonder, however.
Because I gathered them a few days after the rain, then they had to survive the drought period during which I had extreme temperatures (35-37 degrees Celsius). For my region, these are not normal temperatures, they were usually definitely lower.
I will try to see a few fruiting bodies and check the presence of crystals. If I saw them in Excipulum, maybe during cutting I unknowingly moved them there?
Something is wrong with these fruiting bodies! They microscopic very hard because their condition is very weak, so I can't get a clear picture. There is no wonder, however.
Because I gathered them a few days after the rain, then they had to survive the drought period during which I had extreme temperatures (35-37 degrees Celsius). For my region, these are not normal temperatures, they were usually definitely lower.
I will try to see a few fruiting bodies and check the presence of crystals. If I saw them in Excipulum, maybe during cutting I unknowingly moved them there?
L. Winteri I will look for on Phragmetes.
Regards
Mirek
Mirek Gryc,
11-07-2022 19:54
Re : Lachnum on Rubus.
Zotto, as usual, you were right: D
It turns out that I was rinsing the sample too intensively to remove air bubbles. On this occasion, I washed out crystals.
This time I treated the sample more delicate and the effect can be seen in the pictures!
Danke!
It turns out that I was rinsing the sample too intensively to remove air bubbles. On this occasion, I washed out crystals.
This time I treated the sample more delicate and the effect can be seen in the pictures!
Danke!
Hans-Otto Baral,
11-07-2022 20:26
Re : Lachnum on Rubus.
Indeed, this sounds so.