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19-05-2026 19:47

Andreas Millinger Andreas Millinger

Hello dear community,found this species the second

19-05-2026 12:55

Hardware Tony Hardware Tony

After checking Gminder and Otto's library I cannot

19-05-2026 10:27

Patrice TANCHAUD

Bonjour, récolte récente sur terre retournée i

18-05-2026 12:43

Sylvie Le Goff

Bonjour à tousPuis je avoir votre aide sur ce que

19-05-2026 14:56

Åge Oterhals

I found this white cushion-formed ascomycete on ro

18-05-2026 19:49

William Slosse William Slosse

Good evening all,recently I found a portion of a S

18-05-2026 10:13

Lieve Deceuninck Lieve Deceuninck

Dear forum members,I identified this as the teleom

17-05-2026 22:09

éric ROMERO éric ROMERO

Bonjour tous, Je sollicite vos avis pour ce Molli

17-05-2026 19:05

Thomas Flammer

I have found this tiny 200 ym cup shaped apothecia

17-05-2026 16:41

Margot en Geert Vullings

We found this Lachnum on an old Rubus stem.Fruitbo

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Aff. Heterosphaeria on Populus tremula wood
Edvin Johannesen, 13-01-2021 19:14
Edvin JohannesenI though I'd just ask you about this tiny discomycete, which I struggle with. It is only about 0.2-0.4 mm in diameter. It was growing on a Populus tremula log, directly on the wood. The wood is hardly decomposed and very firm. It was collected last May, but I only recently received the dried specimen. It seems like the hymenium in many of the apothecia are now eaten by insects or have decomposed. Thus quite difficult to make nice, thin sections, so I apologise for that.

I am not even sure what family we're in. Initially, when I saw it in wet condition, I thought it was erumpent and was thinking Coccomyces or along those lines. Indeed some of the younger ascomata appear to be erumpent, but in the dried material I see that the dark-rimmed, somewhat dentate apothecia are sitting directly on the wood, anchored by a fringe of white hyphae (anchoring hyphae/subiculum). The hymenium is, when fresh whitish, when dry more greyish and somewhat pruinose.


The spores measure 12-15 x 5 microns, mostly 3-septate, hyaline, smooth. Some spores are smaller (7 x 4) and 1-septate. Many spores are germinating from one or both ends – I guess the specimen is over-mature. What I think are paraphyses are orange-tipped (irregularly flame-like). There are dark, transversely roughened hyphae in the excipulum (fringe) and some spherical cells in what may be subhymenium. The ascus tips are IKI+ (blue in Baral'sche Lösung with no KOH pre-treatment). Structures in the excipulum are stained greenish-blue with IKI, too, but I am not sure that it's due to amyloidity.


Here's a link to some photos. Photo names indicate the medium (KOH, IKI, PC (phase contrast)).


https://www.dropbox.com/sh/4prdmaszwi682ys/AABEAjm33Pf5d4b0lAUx0cnLa?dl=0


The best fit is in the genus Heterosphaeria, but I'm not sure if any species in that genus grows on wood.  Are we in Phacidiaceae?


Suggestions appreciated.  Thanks!  (I hope the link works)

Hans-Otto Baral, 13-01-2021 21:43
Hans-Otto Baral
Re : Aff. Heterosphaeria on Populus tremula wood
Too overmature to say much. Durella (Xylogramma) atrocyanea would be inamyloid. Any idea of the paraphyses?
Edvin Johannesen, 13-01-2021 22:13
Edvin Johannesen
Re : Aff. Heterosphaeria on Populus tremula wood
Difficult to find an intact hymenium. I thought maybe the three phase contrast images showed paraphyses with the golden structures apically. I will try again to see if I can find intact hymenium with paraphyses, but several attempts have failed so far.