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13-06-2025 16:34

Andgelo Mombert Andgelo Mombert

Bonjour,Un petit discomycète qui me résiste. Il

24-07-2025 19:02

Castillo Joseba Castillo Joseba

De esta mañana en tronco caido de Haya A ver si

13-06-2025 09:41

Josep Torres Josep Torres

Hello.A cerebriform ascomycete sprouting scattered

21-07-2025 19:22

Ethan Crenson

Hello all,  Here is an Orbilia found by a friend

14-07-2025 11:20

Michel Hairaud Michel Hairaud

Bonjour, Voici une espèce de  (?) Hyaloscyphace

18-07-2025 23:03

Josep Torres Josep Torres

Hello.Fruitings between 51 and 130 microns in tota

17-07-2025 11:55

Castillo Joseba Castillo Joseba

De ayer en bosque de hayas y abetos, en tieraEjemp

16-07-2025 17:34

Bernard Declercq Bernard Declercq

Hello,I have trouble distinguishing above mention

15-07-2025 13:27

Angel Pintos Angel Pintos

Hello, does anyone have access to the following ar

16-01-2023 21:31

Riet van Oosten Riet van Oosten

Hello, Nearby the find of Calycina claroflava on

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Aff. Heterosphaeria on Populus tremula wood
Edvin Johannesen, 13-01-2021 19:14
Edvin JohannesenI though I'd just ask you about this tiny discomycete, which I struggle with. It is only about 0.2-0.4 mm in diameter. It was growing on a Populus tremula log, directly on the wood. The wood is hardly decomposed and very firm. It was collected last May, but I only recently received the dried specimen. It seems like the hymenium in many of the apothecia are now eaten by insects or have decomposed. Thus quite difficult to make nice, thin sections, so I apologise for that.

I am not even sure what family we're in. Initially, when I saw it in wet condition, I thought it was erumpent and was thinking Coccomyces or along those lines. Indeed some of the younger ascomata appear to be erumpent, but in the dried material I see that the dark-rimmed, somewhat dentate apothecia are sitting directly on the wood, anchored by a fringe of white hyphae (anchoring hyphae/subiculum). The hymenium is, when fresh whitish, when dry more greyish and somewhat pruinose.


The spores measure 12-15 x 5 microns, mostly 3-septate, hyaline, smooth. Some spores are smaller (7 x 4) and 1-septate. Many spores are germinating from one or both ends – I guess the specimen is over-mature. What I think are paraphyses are orange-tipped (irregularly flame-like). There are dark, transversely roughened hyphae in the excipulum (fringe) and some spherical cells in what may be subhymenium. The ascus tips are IKI+ (blue in Baral'sche Lösung with no KOH pre-treatment). Structures in the excipulum are stained greenish-blue with IKI, too, but I am not sure that it's due to amyloidity.


Here's a link to some photos. Photo names indicate the medium (KOH, IKI, PC (phase contrast)).


https://www.dropbox.com/sh/4prdmaszwi682ys/AABEAjm33Pf5d4b0lAUx0cnLa?dl=0


The best fit is in the genus Heterosphaeria, but I'm not sure if any species in that genus grows on wood.  Are we in Phacidiaceae?


Suggestions appreciated.  Thanks!  (I hope the link works)

Hans-Otto Baral, 13-01-2021 21:43
Hans-Otto Baral
Re : Aff. Heterosphaeria on Populus tremula wood
Too overmature to say much. Durella (Xylogramma) atrocyanea would be inamyloid. Any idea of the paraphyses?
Edvin Johannesen, 13-01-2021 22:13
Edvin Johannesen
Re : Aff. Heterosphaeria on Populus tremula wood
Difficult to find an intact hymenium. I thought maybe the three phase contrast images showed paraphyses with the golden structures apically. I will try again to see if I can find intact hymenium with paraphyses, but several attempts have failed so far.