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Hyaloscyphaceae(?) on wood
Juuso Äikäs,
18-01-2020 08:13
Hans-Otto Baral,
18-01-2020 08:21
Re : Hyaloscyphaceae(?) on wood
Seems really anonymous... The hairs are only 2 µm wide as it seems. Doesn't look like a typical Cistella.
Michel Hairaud,
18-01-2020 08:42
Re : Hyaloscyphaceae(?) on wood
Hi Juuso, and Zotto
Seems to belong in Ciliolarina ? (difficult to give a species name, though)
Amitiés. Michel
Kosonen Timo,
18-01-2020 09:48
Re : Hyaloscyphaceae(?) on wood
Hei Juuso,
Keep it in the fridge. I could take a closer look. Is it possible to deliver it fresh to Turku? I am back at the office next Wednesday.
No good suggestions. No match in Hyaloscypha what I can think of. ...well, yes, doesn't really fit Cistella, Psilocistella.. Seppo recently collected something that we thought is Psilocistella obsoleta, but it turned out to be aff Ciliolarina phylogenetically. And with smooth hairs!. It did not have slender hairs like here, so not suggesting this is it. Just saying that someone should take a better a look to Ciliolarina as well :-).
In every case, make careful annotations in the usual reagents (Cotton blue, MLZ, Lugol, water). Always let it "incubate" for a while. Some inclusions might stain. Also important if nothing observed!
bw
Timo
Keep it in the fridge. I could take a closer look. Is it possible to deliver it fresh to Turku? I am back at the office next Wednesday.
No good suggestions. No match in Hyaloscypha what I can think of. ...well, yes, doesn't really fit Cistella, Psilocistella.. Seppo recently collected something that we thought is Psilocistella obsoleta, but it turned out to be aff Ciliolarina phylogenetically. And with smooth hairs!. It did not have slender hairs like here, so not suggesting this is it. Just saying that someone should take a better a look to Ciliolarina as well :-).
In every case, make careful annotations in the usual reagents (Cotton blue, MLZ, Lugol, water). Always let it "incubate" for a while. Some inclusions might stain. Also important if nothing observed!
bw
Timo
Juuso Äikäs,
18-01-2020 17:17
Re : Hyaloscyphaceae(?) on wood
Thank you for the answers. Timo, I sent an email to you.
Kosonen Timo,
23-01-2020 09:28
Re : Hyaloscyphaceae(?) on wood
Hello,
I just studied the sample. It's Ciliolarina.
Apothecia are usually shortly stipitate and the hairs have loosely attached granules/crystals. They are easily blown away even in water mounts just by applying too much pressure. In MLZ/KOH you don't see them at all. The small spore size suggests either neglecta or laetifica. Raitviir (and Huhtinen) give measures of dead spores. Measured in MLZ the spores here are still clearly above 2 um wide (2.5-3.0). ---> C. laetifica. Although, the stipe has some dark pigment suggesting C. neglecta. I have seqs of very small spored samples ("obvious neglecta"). So it will be interesting to compare.
A lovely and plentiful collection!
bw
Timo
Kosonen Timo,
23-01-2020 10:38
Re : Hyaloscyphaceae(?) on wood
One additional comment.
The vast majority of the hairs are apically fairly short-celled (c. 20-30 um long), and thus fit Ciliolarina. Juuso had found some very peculiar aseptate(?) hairs. The paraphyses are usually smooth, so I don't have a definite explanation. ...the majority rules :-)
Timo
Hans-Otto Baral,
23-01-2020 12:22
Re : Hyaloscyphaceae(?) on wood
I agree with C. laetifica. The ascus reaction was supposedly tested in MLZ?
In a collection by Lothar Krieglsteiner (29.XII.2016) the ring reacted dirty red (rb) in Lugol.
A nice feature of this and some other Ciliolarinas is that the surface of free spores stans lilac in CRB (aqueous).
Zotto
Kosonen Timo,
23-01-2020 14:11
Re : Hyaloscyphaceae(?) on wood
yep, ascus apex MLZ negative and red-brown in Lugol. The CRB & Ciliolarina spores I was not aware of.
Timo