Bonjour,

Voici une Helotiale manifestement liée au pyrenomycète Camarosporidiella laburni (det. A. Gardiennet) et qui évoque donc un Unguiculariopsis.

Mais ! Les caractères micro ne sont guère compatibles avec le genre, voire peut être même avec la famille des Cordieritidaceae ??

ApOthecia up to 2,5 mm . gregarious, Ascus H- and IKI - 75-95 µm. PS 30 µm Spores cylindrical to slightly allantoid, OCI 1-1,5 . 11-13 x 2,8-31 µm. PAraphyses rather lanceolate, septate, covered with amorphic hyaline matter , with no VB visible in water but some patches are stained in CRB.

Medulla T. intricata of almost parallel hyphes x 2-3 µm . Ectal of globose cells x6-12 µm covered with amorphic red-brown matter.  Margin hairs up to 150 µm x 3-7 , walls red-brown and granulous as in LAchnum (!) , which can be seen after KOH or in Congo Red. KOH dissolves the brown pigment . No ionomidotic reaction, no cristals.

Toute aide sera bienvenue. Amitiés. Michel

Hans-Otto Baral, 15-04-2019 08:22

Re : Unguiculariopsis-like, but...

Hi Michel
not easy case! But you say the brown pigment dissolves in KOH. If so, it should extrude in the medium, does it? But you say "not ionomidotic"?

could you show some hairs in closeup to better see the warts?

Various characters tell agains Unguiculariopsis, including absence of croziers, warted sublanceolate paraphyses, dissolvable pigment, and hair features. But the family I think is correct.
Zotto

Michel Hairaud, 17-04-2019 09:28

Re : Unguiculariopsis-like, but...

Thanks, Zotto.

As the hairs are strongly agglutinate , I tried KOH to gain visibility but may be the pigments are not actually dissolved. I do not notice that they extrude .

here is a comparison between KOH 5% and tap water. I also tried closeups . These warts seem not firmly fixed to hair walls.

Michel

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Hans-Otto Baral, 17-04-2019 09:40

Re : Unguiculariopsis-like, but...

O.k., so non-ionomidotic. I see a little similarity with what I call "aff. Hyphodiscus" (viridula, "sericea") but which genetically belongs in Cordieritidaceae. From this your fungus differs in wider, almost sublanceolate, rough paraphyses. I think a relationship with this family is quite probable. A genetic result would be interesting.

Michel Hairaud, 20-04-2019 12:36

Re : Unguiculariopsis-like, but...

I wonder why my answer does not appear...

Yes Zotto, the comparison shows similarity.

A sequence is sheduled. Hope we can get a clear result.

THanks again . Michel