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13-01-2026 07:14

Neodasyscypha cerina on indet decorticate log - 303463318
Danny Newman Danny Newman

Neodasyscypha cerina on indet decorticate logThe S

13-01-2026 07:28

Chlorociboria glauca on indet. decorticate hardwood log - 303627368
Danny Newman Danny Newman

Chlorociboria glauca on indet. decorticate logThe

13-01-2026 09:10

Dasyscyphella chrysotexta on indet. decorticate hardwood - 303900282
Danny Newman Danny Newman

Dasyscyphella chrysotexta on indet. decorticate ha

13-01-2026 08:43

Tricladium varicosporioides on indet. decorticate stick - 303997395
Danny Newman Danny Newman

Tricladium varicosporioides on indet. decorticate

13-01-2026 08:49

Coccomyces sp. on fallen Rhododendron leaves - 305874978
Danny Newman Danny Newman

Coccomyces sp. on fallen Rhododendron leavesPretty

12-01-2026 22:02

Coryneum (?) acervuli on oak (?) branch
Ethan Crenson

Hello all, I am hoping someone will have some ins

13-01-2026 07:57

cf. Bombardia on indet. decorticate wood - 304222307
Danny Newman Danny Newman

cf. Bombardia on indet. decorticate woodAppalachia

13-01-2026 07:51

Atrocalyx sp. on indet. herbaceous stem - 303869374
Danny Newman Danny Newman

Atrocalyx sp. on indet. herbaceous stemAppalachian

11-01-2026 20:35

Pyrenomycete on Conifer Wood
Josep Torres Josep Torres

Hello.A very tiny pyrenomycete sprouting sparsely

12-01-2026 05:24

Cyathicula coronata on Urtica dioica - 303628165
Danny Newman Danny Newman

Cyathicula coronata on Urtica dioicaCataloochee Di

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Induction of ascomata in pure culture
Björn Nordén, 07-01-2019 12:40
Hi,

I wonder if anyone has experience of induction of ascomata in pure culture? I suspect the methods are fairly species-specific or are there some protocols involving various media, temperatures and light et c that can be used for screening a lot of various ascomycetes? I found these articles so far:


Houbraken J, Dyer PS (2015): Induction of the Sexual Cycle in Filamentous Ascomycetes... In: van den Berg, MA., Maruthachalam, K (Eds.) (2015): Genetic Transformation Systems in Fungi, Volume 2 (Fungal Biology). Springer. DOI: 10.1007/978-3-319-10503-1_2.


Untereiner Wendy A. (1994): A Simple Method for the in Vitro Production of Pseudothecia in Species of Capronia. Mycologia 86, No. 2 (Mar. - Apr., 1994), pp. 290-295


Grateful for any advice! (for example, lab, person, articles).


Björn Nordén

Spooren Marco, 07-01-2019 14:28
Spooren Marco
Re : Induction of ascomata in pure culture
Hello Björn,

Maby the simplest way to find these things out is to write an e- mail to the Westerdijk-Institute.

I had good advices from the kitchen there, that time, they are an open institute and are willingly to help as much they can, is my expierence.

Otherwise there are some, antiquarian books, but the same Westerdijk-institute has a manual about tese things.

Best,

Marco
Björn Nordén, 07-01-2019 14:36
Re : Induction of ascomata in pure culture
Excellent idea! Thank you!
Paul Cannon, 08-01-2019 12:36
Re : Induction of ascomata in pure culture
Two rather brief general hints here:

1. adding the appropriate sterilized substratum to the agar plate (bark, stem, whatever you isolated from) helps to provide a physical base on which ascomata can develop.

2. Don't overfeed your fungus - culture media with high nutrient levels encourage vegetative growth only. Fungi often produce fruit bodies when stressed, when they "think" that their food source is running out.

But there are about as many methods as there are species, so experimentation is inevitable. And do be patient, sometimes fruit body formation can take months...

Best wishes

Paul