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19-01-2019 18:53

Blasco Rafael Blasco Rafael

Hola, alguno de ustedes conoce este hongo localiza

19-01-2019 19:58

Bernard CLESSE Bernard CLESSE

Bonsoir tout le monde,J'ai trouvé hier, sur branc

18-01-2019 22:14

Edvin Johannesen Edvin Johannesen

Dear all, This nectriaceous species was growing e

18-01-2019 18:17

Lothar Krieglsteiner Lothar Krieglsteiner

... near Spraitbach (Baden-Wuerttemberg n. Schwäb

18-01-2019 09:44

Bernard CLESSE Bernard CLESSE

Bonjour à toutes et tous,J'ai trouvé récemment,

18-01-2019 00:24

laurent magne

bonjour je vous propose ce que je pense etre un un

17-01-2019 20:13

Ethan Crenson

Hello all, Found in Bronx, New York City last wee

17-01-2019 16:10

Peter Thompson

Hello Everyone,Does anyone have a species descript

16-01-2019 23:11

Miguel Ãngel Ribes Miguel Ángel Ribes

Good night friendsDo you know if is there cited Ps

15-01-2019 17:32

Ethan Crenson

Hello, In the Bronx, New York City last Saturday

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Induction of ascomata in pure culture
Björn Nordén, 07-01-2019 12:40
Hi,

I wonder if anyone has experience of induction of ascomata in pure culture? I suspect the methods are fairly species-specific or are there some protocols involving various media, temperatures and light et c that can be used for screening a lot of various ascomycetes? I found these articles so far:


Houbraken J, Dyer PS (2015): Induction of the Sexual Cycle in Filamentous Ascomycetes... In: van den Berg, MA., Maruthachalam, K (Eds.) (2015): Genetic Transformation Systems in Fungi, Volume 2 (Fungal Biology). Springer. DOI: 10.1007/978-3-319-10503-1_2.


Untereiner Wendy A. (1994): A Simple Method for the in Vitro Production of Pseudothecia in Species of Capronia. Mycologia 86, No. 2 (Mar. - Apr., 1994), pp. 290-295


Grateful for any advice! (for example, lab, person, articles).


Björn Nordén

Spooren Marco, 07-01-2019 14:28
Spooren Marco
Re : Induction of ascomata in pure culture
Hello Björn,

Maby the simplest way to find these things out is to write an e- mail to the Westerdijk-Institute.

I had good advices from the kitchen there, that time, they are an open institute and are willingly to help as much they can, is my expierence.

Otherwise there are some, antiquarian books, but the same Westerdijk-institute has a manual about tese things.

Best,

Marco
Björn Nordén, 07-01-2019 14:36
Re : Induction of ascomata in pure culture
Excellent idea! Thank you!
Paul Cannon, 08-01-2019 12:36
Re : Induction of ascomata in pure culture
Two rather brief general hints here:

1. adding the appropriate sterilized substratum to the agar plate (bark, stem, whatever you isolated from) helps to provide a physical base on which ascomata can develop.

2. Don't overfeed your fungus - culture media with high nutrient levels encourage vegetative growth only. Fungi often produce fruit bodies when stressed, when they "think" that their food source is running out.

But there are about as many methods as there are species, so experimentation is inevitable. And do be patient, sometimes fruit body formation can take months...

Best wishes

Paul