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ITS sequences from the following two collections B
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Blasco Rafael
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Hola, He recogido esta muestra de Orbilia sobre Re
Stalked yellowy cup
Malcolm Greaves,
01-10-2018 16:46
Can someone point me in the right area for this yellow/orange stalked cup. It was on a small branch under Fraxinus and Salix. No hairs but almost striate outer surface. No sign of bluing with meltzers and the spores are 13.5 x 4.5.
Thanks
Mal
Hans-Otto Baral,
01-10-2018 16:58
Re : Stalked yellowy cup
This could be Hymenoscyphus consobrinus, but the spores are too unsharp to see welll their contents, and usually this species has an amyloid ring in Lugol.
The paraphyses should be figured (in living state) and also the ascus base checked for croziers.
Zotto
The paraphyses should be figured (in living state) and also the ascus base checked for croziers.
Zotto
Malcolm Greaves,
01-10-2018 22:00
Re : Stalked yellowy cup
Thanks Zotto. I have tried to get better photos showing the relevant details. There seems to be no reaction in the asci with lugol but the paraphyses which are granular did look to develop a slight brown streak. I have shown the base of the asci but again not sure whether it shows a crozier.
Thanks again.
Mal
Hans-Otto Baral,
02-10-2018 07:34
Re : Stalked yellowy cup
Are your pics in Congo red or in another red stain?
Nothing is alive anymore there. But one image shows a splendid crozier. The third pic looks much like a simple-septate ascus, though, maybe only because the other septum is hidden?
When testing iodine, the surrounding, especially the cytoplasm, should take a yellow-brownish colour, otherwise I would say there isn't enough iodine to provoke a reaction.
Zotto
Nothing is alive anymore there. But one image shows a splendid crozier. The third pic looks much like a simple-septate ascus, though, maybe only because the other septum is hidden?
When testing iodine, the surrounding, especially the cytoplasm, should take a yellow-brownish colour, otherwise I would say there isn't enough iodine to provoke a reaction.
Zotto