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14-11-2018 19:07

Andgelo Mombert

Bonsoir,Sur bois mort de feuillu. Apothécies sess

12-12-2018 20:58

Per Marstad Per Marstad

I found this on Fagus. Spores 60-75 x 5 my, with 8

06-12-2018 07:35

Ethan Crenson

Hello all, These were found by my friend recently

08-12-2018 15:13

Per Marstad Per Marstad

This was growing on Fagus, 2-3 mm. The spores are

09-12-2018 23:06

Joop van der Lee

Found on cow dung. Fruitbody is covered with tufs

06-12-2018 00:02

Elsa Sousa

Good evening,I need your sharp eyes on this ascos.

11-12-2018 17:31

Peter Püwert Peter Püwert

Can somebody help ? Massee G. et Salmon E. S. (19

11-12-2018 23:18

Iglesias Plácido

Hola amigos, est sortie la revue Errotari de cette

10-12-2018 23:47

BERNARD CLESSE BERNARD CLESSE

Que pensez-vous de cette récolte, faite sur crott

07-12-2018 17:24

Enrique Rubio Enrique Rubio

Quelqu'un pourrait-il avoir une copie de ce docume

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Scutellinia dont les collections n'ont pas sporulé
Claude Kaufholtz-Couture, 13-01-2018 13:46
Claude Kaufholtz-CoutureBonjour à tous,

J'ai deux autres collections de Scutellinia, mais elles n'ont pas sporulé. J'imagine que l'observation et la mesure des ascospores ne seront pas aussi précises que si obtenues par sporulation. Mais en étudiant l'hyménium, est-il possible d'arriver à une certaine précision ? Est-ce un exercice trop hasardeux ?
Merci de vos commentaires de formation...
Meilleures salutations,


Hello everyone,

I have two other collections of Scutellinia, but they have not spore print. I imagine that the observation and measurement of ascospores will not be as accurate as if obtained by sporulation. But when studying the hymenium, is it possible to reach a certain precision? Is it a risky exercise?
Thank you for your training comments ...

Best regards,
Claude

Malcolm Greaves, 13-01-2018 15:45
Malcolm  Greaves
Re : Scutellinia dont les collections n'ont pas sporulé
Bonjour Claude
With many ascos as the spores develop in the asci they grow until mature so taking measurements of immature spores will give you inaccurate figures. The added problem with Scutellinia is that some of the spores shrink in size as the outer layer of the spore coalesces to become the ornamentation(up to 6µ shorter according to Schumacher). With some species if the spores have almost matured they will take up the stain even whilst still in the asci and using that information and the details of the hairs you can come to a reasonable conclusion. Another proviso given by Schumacher is that imature spores can show more or less ornamentation than ejected spores.
So you can give identification a go with your immature specimens but the chances of success are lower.
Mal
Viktorie Halasu, 13-01-2018 18:18
Viktorie Halasu
Re : Scutellinia dont les collections n'ont pas sporulé
Hello Claude,

you can check, if there's any leaf, piece of wood etc. over the hymenium of some apothecia - the sporeprint might be on the lower side of it. I saw this once with S. cejpii, one apothecium was dried with a fragment of a leaf on it - after removing it, it was white from the lower side with lots of nice mature spores.
Also, if the spores in asci near the margin are not completely mature, you might try to take a small sample from the centre of the hymenium. Often there's higher percentage of mature spores there than near the margin. I got this advice from another mycologist, who's working a lot with Scutellinia and I can confirm that it worked for me too. But I used it only with a few collections, because taking a sample even with thin and sharp injection needle makes further damage around the puncture and about a quarter of the spores in the sample were crushed. Maybe would I have cut the whole apothecium in half and then made a thin section from the middle, it would be less damaging, but I didn't try that yet. 
With my own collections I always try to get sporeprint on aluminium foil (a piece of dark paper would be perhaps better?), which I later pack together with the exsiccate. 

Kind regards,
Viktorie 
Claude Kaufholtz-Couture, 14-01-2018 13:01
Claude Kaufholtz-Couture
Re : Scutellinia dont les collections n'ont pas sporulé
Hello Mal,

I take good note of everything you just explained to me and I will try, knowing that my chances are rather slim.
Best
Claude

Claude Kaufholtz-Couture, 14-01-2018 14:05
Claude Kaufholtz-Couture
Re : Scutellinia dont les collections n'ont pas sporulé
Hello Viktorie,

The idea of looking around the collection on a debris is very interesting and I will look carefully. For my part, I cut the Scutellinia in four, then make a thin cut from the middle to the margin; Finally, I will see my future collections. For spore print, I usually put on a transparent plastic sheet protector, which I then cover. Eight times out of ten, I get a nice spore; which is essential for measurements and observation of ornaments.


Best regards,
Claude