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18-12-2017 10:41

Per Marstad Per Marstad

Hello.I would like a name on this.Spores 15x6 my.A

18-12-2017 12:24

Pauline. Penna

Bonjour tousThese tiny Xylaria were found on a fal

17-12-2017 10:54

Per Marstad Per Marstad

Hello.This was found on sticks of Fagus.Spores 11-

17-12-2017 13:17

Blasco Rafael Blasco Rafael

Hola, de nuevo necesito su ayuda con esta muestra,

17-12-2017 12:55

Blasco Rafael Blasco Rafael

Hola, les parece que sea la que propongo ????Recog

16-12-2017 09:57

Castillo Joseba Castillo Joseba

Me mandas esta muestra seca en caña de PhytolacaL

16-12-2017 14:18

Per Marstad Per Marstad

I would like a name on this. 2 mm in diameter, dar

16-12-2017 20:58

Mlcoch Patrik Mlcoch Patrik

Good day,I want ask you about help with determinat

15-12-2017 15:20

Bernard CLESSE Bernard CLESSE

Dans le même xérobrometum (pelouse calcicole xé

14-12-2017 11:51

Per Marstad Per Marstad

3-8 mm in diameter, black, sp. 14-15 x 7-8 my, asc

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Neurospora from used coffee filter bags
Stephen Martin Mifsud, 09-12-2017 17:43
Stephen Martin MifsudMy friend was concerned about a fungus growing on uses coffee filter bags which had this attractive salmon-peach colour. He sent me a sample by post. Under the microscope, it turned out to be a yeast - likely a member in the Saccharomycodaceae family. I think yeast ID is not easy or at least I have not found any convenient key to families and genera. If this can be easily identified let me know but I think it requires chemical tests and nowadays molecular techniques.

Well if this is a health hazard for my friend please let me know to advise him to sterilise his coffee maker.
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David Malloch, 09-12-2017 18:34
David Malloch
Re : Yeast (Saccharomycodaceae?) from used coffee filter bags
Hello Stephen,

This may be a species of Neurospora such as N. sitophila, known under the anamorphic names of Chrysonilia sitophila or Monilia sitophila.  Your second photo seems to show intact conidiophores.  When you make a mount of N. sitophila the conidial chains separate out into one-celled units.  Conidiogenesis is blastic with the youngest conidium at the apex of the chain.

Your friend should probably not worry about sterilizing the coffee maker but perhaps might discard the bags before the fungi find them.

Dave
Stephen Martin Mifsud, 09-12-2017 19:06
Stephen Martin Mifsud
Re : Yeast (Saccharomycodaceae?) from used coffee filter bags
Dave thank very much you for your input - greatly appreciated!!! You are absolutely right, I was deceived but the yeast-like budding but hopefully I would not be deceived again by this genus!

http://old.vscht.cz/obsah/fakulty/fpbt/ostatni/miniatlas/images/plisne/mikro/Chrysonilia%20sitophila%20CCF%2018%20mikro.jpg

The link above shows the similarity of this Neurospora.  I have inoculated it on PDA and maybe I can analyse better the conidiogenesis apparatus. 


Just a question: Within the salmon-pink mass, there are white filamentous branching structures - do you these are immature structures of the same species or a second species?

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David Malloch, 09-12-2017 19:25
David Malloch
Re : Yeast (Saccharomycodaceae?) from used coffee filter bags
Perhaps the growth on the coffee filter was a little old. I suspect that the structures you saw are the remains of the conidiophores and conidiogenous cells.

By the way; be careful with those cultures.  The conidia of Neurospora sitophila become airbore with the slightest movement of air and can infect all your other cultures.  It is a terrible weed that can take over a lab. Most mycologists are reluctant to evem open a plate where they suspect it is growing.

Dave
Stephen Martin Mifsud, 09-12-2017 19:34
Stephen Martin Mifsud
Re : Neurospora from used coffee filter bags
Yes, they were old filters. I am culturing them at home and we are going to move out in 5 months so contamination is least concern, but then I will not take the plates in my Lab at work. My goal is to try a tape mount maybe I see better intact structures and photograph them.