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Lasiosphaeria or allied genus ?
Vivien Hodge, 12-03-2017 21:53
Hello Forum

This fungus was growing on an attached dead branch of Quercus robur in a small wooded area in Capel, Surrey, England. Lat, long 51.151143, 0.313536, altitude 85m.

Perithecia were erumpent to superficial. Individual perithecia were about 0.25mm. in diameter, these occurred in various quantities from very small to a group of 1 cm across shown in 1st and 2nd photos. Peridium was textura angularis.


Most of the perithecia contained immature spores, these were golden, 35-37.5 x 5-7.5 and each had a very conspicuous nucleus. A few mature spores were found, these were 6-7 septate and averaged 41 x 7.5. The septae seemed to develop late and were not observed when the spores were within the asci. Asci on average were 150 x 15.


Some of the ascospores had conidia developing at the septae, the conidiospores were 10-12 x 2.5-3.5. Golden masses of spores were exuding from some of the ostioles, these consisted of both conidio and asco spores.


It seems to resemble a Lasiosphaeria or an allied genus but I cannot get any closer with its determination, can anyone help please?

Best wishes
Vivien 

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Hans-Otto Baral, 12-03-2017 22:02
Hans-Otto Baral
Re : Lasiosphaeria or allied genus ?
This is one I think I know: Lasiosphaeria sorbina.
Jacques Fournier, 12-03-2017 22:40
Jacques Fournier
Re : Lasiosphaeria or allied genus ?
Hello Vivien,
likely L. sorbina, as pointed out by Zotto.
You should check your collection against the detailed description given by Miller & Huhndorf in Mycologia, 96(5), 2004, pp. 1106–1127.
Cheers,
Jacques
Andrew N. Miller, 12-03-2017 23:18
Vivien Hodge, 13-03-2017 00:04
Re : Lasiosphaeria or allied genus ?
Thank you Zotto, Jacques and Andy for the quick responses, and thanks Andy for the PDF.

I have checked my collection and it does indeed seem consistent with the description of Lasiosphaeria sorbina given in the Miller & Huhndor paper that you refer to. The only slight difference is that the spores in my collection appeared golden particularly when in the ascus, could that just have been due to pigments present in the centrum?

It is apparently a very rare, or at least rarely recorded, fungus here in the UK so great to have it identified. 

Best wishes
Vivien
Andrew N. Miller, 13-03-2017 00:09
Andrew N. Miller
Re : Lasiosphaeria or allied genus ?
Yes, true Lasiosphaeria's mostly have yellow centrum pigments that can stain the asci and ascospores a yellowish color.

Cheers,
Andy
Vivien Hodge, 13-03-2017 00:15
Re : Lasiosphaeria or allied genus ?
Thanks Andy, that's reassuring.
Cheers
Vivien
Alain GARDIENNET, 13-03-2017 07:58
Alain GARDIENNET
Re : Lasiosphaeria or allied genus ?

An anecdote, I found it a new time yesterday, on Sorbus aria, around Eutypella sorbi, hidden under the bark. So I agree with the identification , of course. A nice fungus.


Alain

Hans-Otto Baral, 13-03-2017 09:01
Hans-Otto Baral
Re : Lasiosphaeria or allied genus ?
I have some problems to distinguish L. capitata. The main difference seems to be the yellowish colour of the spores against a pinkish colour in L. sorbina. A whitish surface on the perithecia of L. sorbina I cannot see. Perhaps L. sorbina does not get warted spores when overmature?

I actually saw the paratype of L. capitata, probably given to me by Jacques in 2004 for some reason.

In GenBank I only see L. sorbina, does a sequence of L. capitata exist?

I still wonder about a collection on Fagus bark (inner side), with light brown Sp. *19.5-23 x (5-6-)6.5-7 µm. - Munk reports L. sorbina on inner side of Fagus bark, but with sp. 28-45 x 6-8 µm.

Zotto
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Andrew N. Miller, 13-03-2017 15:48
Andrew N. Miller
Re : Lasiosphaeria or allied genus ?
No, no sequence of L. capitata exists.  I would be happy to generate a sequence if someone can send me material.

Cheers,
Andy