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Thomas Læssøehttps://svampe.databasen.org/observations/10527903
On Populus sp. bark
Elsa Sousa,
04-02-2017 21:02
I need some help here because I just don't know where to begin...
Ascomata ±102, dried, collected some months ago, almost one year.
Spores 27.8-35.2 x 5-6.5
Me 31.9x5.7 4(?)-7 septate
Ascus 115.6x17.76
Paraphyses filiform some with slightly enlarged tips
Thank you,
Elsa
iodine reaction
IMGP0140-2--0001.JPG
Hans-Otto Baral,
04-02-2017 21:14
Re : On Populus sp. bark
This requires the iodine reaction of the ascus tip and a picture of the excipulum, in section or surface view. At present I have no idea. The substrate looks a bit lke wood, is it decayed bark, perhaps inner side of bark? On the moist ground?
Which country did you collect it?
Which country did you collect it?
Elsa Sousa,
04-02-2017 22:11
Re : On Populus sp. bark
Hi Otto, thank you for your answer
I was afraid somebody would ask me for iodine reaction...Mine finished, so... I could try with 2 options, iodopovidona 10% iodine, or one solution with 6 g iodine + 2g potassium iodide + etanol 70º /100ml. What do you think is the best?
About the collect: here in Portugal Braga. Wet place, inner bark of populus (fallen).
Elsa
Hans-Otto Baral,
04-02-2017 22:59
Re : On Populus sp. bark
I think you can test the first one, maybe it works.
Elsa Sousa,
04-02-2017 23:04
Re : On Populus sp. bark
Sorry Otto,
I missed up all these...I was trying to spare the apos and choose another which were close. I thought they were the same, a bit more dried. So, I'm going to correct the macro photos, and add the blue tip of iodine reaction. Later I think about the other, definitely completely different.
Elsa
I missed up all these...I was trying to spare the apos and choose another which were close. I thought they were the same, a bit more dried. So, I'm going to correct the macro photos, and add the blue tip of iodine reaction. Later I think about the other, definitely completely different.
Elsa
Hans-Otto Baral,
04-02-2017 23:12
Re : On Populus sp. bark
With dead material it is very very complicated to work.
Elsa Sousa,
04-02-2017 23:30
Re : On Populus sp. bark
Otto,
Sorry for my ignorance, but should dead material have still complete spores inside asci?
Thank you,
Elsa
Sorry for my ignorance, but should dead material have still complete spores inside asci?
Thank you,
Elsa
Hans-Otto Baral,
04-02-2017 23:39
Re : On Populus sp. bark
surely, but sometimes many spores get ejected during the drying process, and less mature spores remain in the asci. Anyway, you lose a lot of characters in the dead state.
Elsa Sousa,
04-02-2017 23:50
Re : On Populus sp. bark
Dear Otto,
Thank you for your patience. After some search, I think this is Durella connivens.
https://www.asturnatura.com/fotografia/setas-hongos/durella-connivens-fr-rehm-2/2493.html
Do you agree?
Thank you for your patience. After some search, I think this is Durella connivens.
https://www.asturnatura.com/fotografia/setas-hongos/durella-connivens-fr-rehm-2/2493.html
Do you agree?
Hans-Otto Baral,
05-02-2017 09:58
Re : On Populus sp. bark
I actually thought about this species, but to affirm it you should simply put an apothecium upside down on a slide and press with the cober slip. Then you see the brown excipulum which must show a parallel (radial) hyphal structure.
Elsa Sousa,
05-02-2017 11:14
Re : On Populus sp. bark
I have 2 bad pictures of that feature, one worse than the other. Maybe the excipulum wasn't enough wetted and/or I scratched the slides too much so it broke. However, I think that it could be D. connivens.
Hans-Otto Baral,
05-02-2017 11:23
Re : On Populus sp. bark
It's not clearly seen but I still doubt Durella. D. connivens has an inamyloid hemispherical ascus apex.
The conidia in your last picture belong to a hypho, I suppose Cryptocoryneum.
The conidia in your last picture belong to a hypho, I suppose Cryptocoryneum.
Elsa Sousa,
05-02-2017 11:54
Re : On Populus sp. bark
Thank you Otto,
Now I have that doubt too. Maybe what I saw isn't a blue reaction, because the second photo already shows a dark zone on ascus apex without iodine, just water.
Now I have that doubt too. Maybe what I saw isn't a blue reaction, because the second photo already shows a dark zone on ascus apex without iodine, just water.