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Calycina subtilis?
Steve Clements, 30-04-2016 13:03
Bonjour,
On a trouvé cette petite Disco sur une aiguille de pin. Il est si petit que je ne suis pas sur si mon microscope est assez bon pour le travail!
Hi,
We found this on pine needles in the Peak District, N. England, recently. It was very small and really I felt that my microscope wasn't quite up to it. However, I got the following features:
Disc whitish, "folding" when dry. Approx 0.2 - 0.3mm diameter.
Stalk about 0.1mm long.
Spores approx 5 x 1.5
Asci approx 30 x 4. Microscopy was difficult as the cells didn't disperse nicely. Tips appear to blue in Meltzers (but the annoyingly blue LED light makes lots of things look blue).
"Hairs" on the outside of the cups were approx 15 x 5.

From Ellis and Ellis I found Pezizella subtilis listed for pine needles, and it fits ......just about.
But there are only 17 British (FRDBI) records. It's now called Calycina subtilis.
I feel sure there is likely to be a much more sensible ID.
Steve

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Stip Helleman, 30-04-2016 14:12
Stip Helleman
Re : Calycina subtilis?
Hallo Steve,
could it be Cistella acuum? The hairs are apically warted, what suggests Cistella.

cheers,
Stip
Hans-Otto Baral, 30-04-2016 19:46
Hans-Otto Baral
Re : Calycina subtilis?
The warted hairs exclude C. subtilis. The only species I know on conifers with such hairs is "Pezizella" junipericola, a Calycina related to C. gemmarum (= Gemmina gemmarum).

Both species have a distinct tendency to form 4 smaller spores in the ascus (heterospory), could you observe this?

Zotto
Steve Clements, 01-05-2016 14:49
Re : Calycina subtilis?
Hi,
Than you both for your kind help.
I didn't notice heterospory - but I wasn't looking for it.
Unfortunately I have lost the pine needle - but we can try to find more on Thursday.
best regards,
Steve