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Mollisia or alike?
Lepista Zacarias,
29-03-2016 14:32
I found this cups growing on the bark of Eucalyptus. It seems to be Mollisia or close. However, the spores are a bit to large. I got the following dimensions for them:
(9.7) 9.8 - 14.1 (14.6) × (2.2) 2.4 - 3.7 (3.9) µm
Q = (2.8) 3.1 - 4.4 (4.6) ; N = 23
Me = 11.9 × 3.3 µm ; Qe = 3.7 .
I attach a set of photos with its features.
I would like to have some help for its classification.
Thanks in advance,
zaca
Andreas Gminder,
29-03-2016 14:38
Re : Mollisia or alike?
Hello,
the spores wouldn't rule out Mollisia, but all the rest doesn't fit.
I think this is a Hymenoscyphus or a related genus.
best regards,
Andreas
Lepista Zacarias,
29-03-2016 14:44
Re : Mollisia or alike?
Thanks, Andreas, for your opinion.
Let's move the target genus.
Best regards,
zaca
Let's move the target genus.
Best regards,
zaca
Hans-Otto Baral,
29-03-2016 14:49
Re : Mollisia or alike?
Regrettably all in dead state. Important would be the shape of the amyloid apical ring. Is the excipulum gelatinized?
Zotto
Zotto
Andreas Gminder,
29-03-2016 15:10
Re : Mollisia or alike?
Hello,
may be it's worth comparing with Hymenoscyphus phialea.
best regards,
Andreas
Michel Hairaud,
29-03-2016 19:18
Re : Mollisia or alike?
Bonjour,
I would suggest a comparison with Bisporella subpallida, at least the macro would fit and also the excipulum ?
Amitiés
Michel
Hans-Otto Baral,
29-03-2016 19:57
Re : Mollisia or alike?
This was also my first idea but the spores are much too large and contain too much oil.
Lepista Zacarias,
29-03-2016 22:03
Re : Mollisia or alike?
Dears Zotto, Andreas and Michel,
Many thanks for your comments, opinions and proposals.
I'm a bit confused by now: I could not find any information about Hymenoscyphus phialea, suggested by Andreas, and regarding Bisporella subpallida, mentioned by Michel and Zotto, that species has spores much smaller than my specimen, despite of the similar macro appearance, as I could see in several places. I checked again the dimensions of the spores.
I take the oportunity to upload two photos, one of the ectal exciple and the other with some strange hyphae seeming incrusted.
Regards,
zaca
Many thanks for your comments, opinions and proposals.
I'm a bit confused by now: I could not find any information about Hymenoscyphus phialea, suggested by Andreas, and regarding Bisporella subpallida, mentioned by Michel and Zotto, that species has spores much smaller than my specimen, despite of the similar macro appearance, as I could see in several places. I checked again the dimensions of the spores.
I take the oportunity to upload two photos, one of the ectal exciple and the other with some strange hyphae seeming incrusted.
Regards,
zaca
Hans-Otto Baral,
29-03-2016 22:08
Re : Mollisia or alike?
o.k., the excipulum looks lke a thin-walled t. angularis. The warted hyphae I am not sure if they belong to the fungus.
The asci look amyloid, do they? The shape of ring would really be helpful.
The correct name for Andreas' suggestion is Helotium phiala, but that species has inamyloid asci.
The asci look amyloid, do they? The shape of ring would really be helpful.
The correct name for Andreas' suggestion is Helotium phiala, but that species has inamyloid asci.
Lepista Zacarias,
29-03-2016 23:13
Re : Mollisia or alike?
Thanks again, Zotto, for your interest and for sharing your knowledge.
I will repeat the microscopy and if something new I will post it here. As far as I can see from the previous micro, it is difficult to classify the asci as amyloid since there is a very diffuse bluish tone at the apex and that's all, but if you see the photo in water the appex already seems a bit blue. I also took a photo, that I upload now, of the Lugol reaction in a mount with KOH and that gave blue apex.
Best regards, zaca
I will repeat the microscopy and if something new I will post it here. As far as I can see from the previous micro, it is difficult to classify the asci as amyloid since there is a very diffuse bluish tone at the apex and that's all, but if you see the photo in water the appex already seems a bit blue. I also took a photo, that I upload now, of the Lugol reaction in a mount with KOH and that gave blue apex.
Best regards, zaca
Hans-Otto Baral,
30-03-2016 09:55
Re : Mollisia or alike?
yeah, this is good! It excludes Hymenoscyphus and also Bisporella subpallida or pallescens. This is the Calycina-type which occurs also in "Bisporella" citrina and allies, but since your excipulum is thin-walled I would exclude any taxon previously placed in Bisporella.
If you find the fungus again, please make preparations in water with only slight pressure on the cover slip. maybe we can then proceed in finding a genus.
If you find the fungus again, please make preparations in water with only slight pressure on the cover slip. maybe we can then proceed in finding a genus.
Lepista Zacarias,
30-03-2016 23:33
Re : Mollisia or alike?
UPDATE
Following the recommendations of Zotto I remade the microscopic observation, using a mount in water to observe the spores; After joined IKI and analyzed the evolution of the reaction, taking photos at regular intervals. As expected from the previous observation, the bluish apex reaction of asci is very discrete. I attach some photos, hoping that these can be useful.
Following the recommendations of Zotto I remade the microscopic observation, using a mount in water to observe the spores; After joined IKI and analyzed the evolution of the reaction, taking photos at regular intervals. As expected from the previous observation, the bluish apex reaction of asci is very discrete. I attach some photos, hoping that these can be useful.
