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Sur Rubus
Camille Mertens,
17-02-2016 16:26
Récolté sur Rubus fructicosus le 4 février 2016.
Diam. 0.5 mm.
Sessile, tôt pulviné, blanchâtre, rougissant et enfin brunissant.
Asques octosporés avec crochets : 85 - 100 x 9,4 - 13, IKI +
Spores fusiformes multiguttulées, comportant une cloison à mâturité, enrobées d'une gaine: (19,3) 21,4-23,4 (24,6) x (4,5) 4,7 - 5 µm
Paraphyses contenant des vacuoles réfringentes.
Quelques poils épars, hyalins.
J'ai bien une idée mais cela ne correspond pas macroscopiquement.
Merci de votre aide.
Camille
Hans-Otto Baral,
17-02-2016 16:37

Re : Sur Rubus
Dear Camille
The endless Calycina saga, and an interesting species that I do no know.
I think the "poils" are ascogenous hyphae.
Some Calycinas show such reddening indeed.
my C. subparilis is similar but has smaller spores and an IKI-red apical ring.
To see the ring-shape better you should take a photo of a dead ascus.
Is it from Belgium?
Zotto
The endless Calycina saga, and an interesting species that I do no know.
I think the "poils" are ascogenous hyphae.
Some Calycinas show such reddening indeed.
my C. subparilis is similar but has smaller spores and an IKI-red apical ring.
To see the ring-shape better you should take a photo of a dead ascus.
Is it from Belgium?
Zotto
Camille Mertens,
17-02-2016 17:09
Re : Sur Rubus
Dear Zotto.
Vielen Dank fur ihrer Anwort.
The collection comes indeed from Belgium (South of Brussels).
How could I "kill" the ascus? Using Melzer?
Kind regards.
Camille
Vielen Dank fur ihrer Anwort.
The collection comes indeed from Belgium (South of Brussels).
How could I "kill" the ascus? Using Melzer?
Kind regards.
Camille
Hans-Otto Baral,
17-02-2016 17:42

Re : Sur Rubus
for example by stronger pressure on the cover slip, this is the easiest way. Or when you wait some minutes after adding Lugol. Also by drying the preparation and later adding water may kill the asci.
Camille Mertens,
17-02-2016 19:06
Hans-Otto Baral,
17-02-2016 21:22

Re : Sur Rubus
Yes, this is helpful, you see the ring and apical wall around is thicker, and the cytoplasm detached.
But with so large spores I know nothing.
But with so large spores I know nothing.
Camille Mertens,
17-02-2016 22:52
Re : Sur Rubus
Many thanks for your help Zotto.
Camille