Regarde là : http://www.pilzforum.eu/board/thema-spumatoria-longicollis, je pense que ta
récolte correspond !

Michel.

These collections, including the one of Michel at http://www.pilzforum.eu/board/thema-spumatoria-longicollis, seem very close to species of the genus Kathistes.  The attached photos are of Kathistes calyculata, collected on moose dung in New Brunswick, Canada. If anyone has recent material of S. longicollis I would be very grateful to receive a few perithecia for sequencing.

The panel in the right of the photo show one sporidioma of K, calyculata.  The function of these structures is unknown, but they seem to be a constant feature of species of Kathistes.

David

Yes, like Michel says, I also think you've found another time the rare Spumatoria longicollis!
Very nice found, Joop!
We discussed it three years ago here:

www.ascofrance.fr/search_forum/22944

Norbert

Dave, you wrote about Kathistes calyculata.

"These collections, including the one of Michel at http://www.pilzforum.eu/board/thema-spumatoria-longicollis, seem very close to species of the genus Kathistes. The attached photos are of Kathistes calyculata, collected on moose dung in New Brunswick, Canada"

I've never heard about this species!!!
But it seems very closely to Spumatoria.
In 1990 you published this species in Can. J. Bot. 68(8).

It would be very great, if you would send a PDF to compare!

Thanks in advance.

Norbert

Hi Norbert,

It's in your email.

Dave

Thank you, Dave, you are fast!
Give me some minutes to compare! ;-)
Maybe tomorrow.

Norbert

Hello David,

Then I also have the PDF?
Thank you very much.

Michel.

Hi Michel..

Check your email.

Dave

Super!

Merci David.

Michel.

Is it possible to send to me too? Thanks :)

Regards
björn

Hi Björn,

In your email.

Dave

Here we have the asci.
The ascus has the shape of a waterbag like the one they use during a camel ride.
It has two extension of which one is extending in water, when young it only has one.
Containing 8 spores in 2 bundles of 4.
Young ascus: 58.6x21.2 um.
Mature ascus: 80x-29.5 um.

I also observed a perithecia with two necks and another one with a single neck and two beaks.

With this ascus- and sporesize and the numerous small oildrops in vital ascospores as well as the missing of sporidiomata this is Spumatoria longicollis.
But interesting to know, that the genus Kathistes is simular to Spumatoria.
We'll do our best to find one or the other species of Kathistes in the future.

And be sure, when I find Spumatoria at the next time, I will send it to you for sequencing, Dave.

Norbert

Hello Norbert,

 

I already did send David about 10 species for sequensing today.

Joop

Well done, Joop!

Hi Joop and Norbert..

I have had success finding these fungi on dung of moose (Alces alces), but that is because it is the common large animal where I live.  Other dung types are probably just as productive.

Species of Kathistes, Pyxidiophora and Sphaeronaemella are all long-necked ascomycetes common on moose dung. Probably on deer, cow and horse as well. The trick for finding them is to collect dung that is very fresh; probably on the ground only for a day or so. They must have have been visited by insects. Don't dry the dung, but put it directly in a moist chamber for incubation. Don't let it get too warm or Trichoderma will take over. Probably 15° to 18°C is best. Examine with a dissecting microscope at fairly high magnification. Pyxidiophora species will have prominent anamorphs that may offer a sign of their presence.

Good luck in your searches for these interesting ascomycetes!

Dave

Hello Dave, thank you for all these details very interesting, I'll also look in my area.

Michel.

Hello David,

Good to know that the dung has to be very fresh. The chunck of cow dung was too fresh so I kept it in a plastic box covered by a transparant lit with a temperature between 16-18 deg. C., and no direct sunlight.
I checked it every two days for pyrenomycetes and the first fungi to appear after one week were Coprinus species mostly C. stercorius, it took 3 weeks before I discovered S. longicollis with 100+ species, together with different kinds of Podospora and Sporormiella species.

The Pyxidiophora basidiorostris and Pyxidiophora spec. I found were problably also produced on very fresh dung.

A better picture of the species on the cap of Pilobus

Miniscule!

Hello Norbert,

David mailed me that he had found sporidiomata in the surrounding dung.
Compairing the photos he did send me I realized that I found one myself not knowing that it was a sporidioma