El que las ascas sean totalmente amiloides me hace pensar en que es un liquen, pero la total ausencia de talo me hace dudar, ¿Qué opináis vosotros? Gracias.


Ascomas negros, discoides, no estipitados, hasta de 1,2 mm de diámetro, que en contacto con KOH 5% reaccionan desprendiendo una pigmentación amarillo-anaranjada. Ascas cilíndricas, afinándose hacia la base, octospóricas, unitunicadas, sin aparato apical, que produce la descarga por una rotura del ápice, euamiloides en toda su longitud y sin croziers  en la base, de (71-)77-92(-99) x (7,0-)7,9-8,6(-9,3) µm, N = 15, Me = 84 x 8,2 µm. Ascosporas biseriadas en la parte superior del asca, cilíndricas, curvadas o rara vez rectas, lisas e hialinas, sin contenido de aceite o con algunas pequeñas gútulas en los extremos, unicelulares o con un septo cuando están muy maduras, que parecen desacerse en ascocondios finalmente, de (7,7-)9,6-14,7(-15,8) x (2,4-)2,7-3,2(-3,6) µm, Q = (2,5-)3,3-5,4(-5,7), N = 52, Me = 12,2 x 2,9 µm, Qe = 4,2. Parafisis hialinas, multiseptadas, constreñidas en los septos, con algunas pequeñas gútulas de pigmentación amarillenta en su interior, ramificadas en el ápice, donde están cubiertas por una masa marrón oscuro o casi negra. 

Salvador. 

Hans-Otto Baral, 28-06-2015 10:26

Re : Discomycete negro ¿liquen?

Very interesting collection! Not sure if the asci are without croziers, are you? Is there a clearar photo about this feature?

And how sure are you with the substrate? Did you check the wood anatomy? Because your sample closely concurs with Patinella abietina (see my Durella folder, subfolder Patinella aff., on Pinus and Abies), including the amyloid asci and yellow KOH-reaction. But asci and spores are larger in your sample.

So it is possibly a different species of a possibly undescribed genus.

I compared Dermateopsis because of a similar excipulum, but that has +/- fusiform and asci only bluing at the top, also unbranched paraphyses..

Hans-Otto Baral, 29-06-2015 08:42

Re : Discomycete negro ¿liquen?

yes, celarly with croziers. This is only a matter of having free ascus bases in good orientation, then it would be very clear.

I hope you have these pics also without the black circle. This might be a new species and the photos are valuable.

It would be great if this sample could be sequenced because we have no idea where it belongs. Perhaps in the Tympanidaceae. Do you have enough apothecia?

I suggest you could send a part of it, for example, to Guy Marson.

Zotto

Hans-Otto Baral, 29-06-2015 17:41

Re : Discomycete negro ¿liquen?

Ah, i did not yet look through all these many images, sorry.

Here is Guy's address:

Guy Colling c/o Guy Marson
Musee National d´Histoire Naturelle
25 rue Münster
L-2160 Luxembourg
gmarson@pt.lu

Thank you very much!

Zotto

Hans-Otto Baral, 18-07-2015 10:21

Re : Discomycete negro ¿liquen?

That P. abietina has probably nothing to do with P. hyalophaea was already rather clear from its morphology, especially the entirely amyloid asci. Now it is easy to see with Guy's sequence that it is related to lichen genera rather than helotialean ones, which is quite a surprize. You must simply copy the sequence to GenBank (BLAST)
http://blast.ncbi.nlm.nih.gov/Blast.cgi?PROGRAM=blastn&BLAST_PROGRAMS=megaBlast&PAGE_TYPE=BlastSearch&SHOW_DEFAULTS=on&LINK_LOC=blasthome

press BLAST and within 10 sec. you get the result. It is mainly Umbilicaria what appears.

Yes, Guy, when you send me back my samples you can add this one.

Zotto

Hans-Otto Baral, 19-07-2015 17:09

Re : Discomycete negro ¿liquen?

Surely your fungus is not P. abietina but a closely related, perhaps new species.

Yes, this is surprizing that a lichen with a very conspicouos, foliose thallus shows genetical resemblance to a species without thallus. I do not know Umbilicaria well, and I do not find good photos of asci in the internet. The apothecia look indeed similar to this Patinella.
Would be interesting to know whether Umbilicaria shows any KOH-soluble pigments.

GenBank: Did you see the rectangle on the uper left where to paste the sequence, over which stands:
Enter accession number(s), gi(s), or FASTA sequence(s)?

Zotto