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Greyish on Rubus leaves
Marja Pennanen,
23-06-2015 08:50
the summer finally started here.
These change colour from greyish to lighter and are about 0,3 mm wide,
The spores are multiguttulate, 10-12x3-4.
The asci are about 55-80x9-11 and weakly IKI blue.
Paraphyses are about 3 micrometers wide,
Sorry, but I could not distinguish, weather the asci tips were with Hymenoscyphus type colouring.
Help is surely needed with this and some other collections, too...
Marja
Hans-Otto Baral,
23-06-2015 09:10
Re : Greyish on Rubus leaves
Hi Marja
this should be a Calycellina, and the spores remind me of C. lutea on Rhododendron. But those are yellow and have pointed hairs at the margin. Yours is apparently without any hairs? Not even protuberances of a Mollisina?
Zotto
this should be a Calycellina, and the spores remind me of C. lutea on Rhododendron. But those are yellow and have pointed hairs at the margin. Yours is apparently without any hairs? Not even protuberances of a Mollisina?
Zotto
Marja Pennanen,
23-06-2015 09:27
Re : Greyish on Rubus leaves
Dear Zotto,
Not Mollisina, but the marginal cells had some vacuoles.
Marja
Forgot, that the paraphyses are septate and have a vacuole at the tip.
Not Mollisina, but the marginal cells had some vacuoles.
Marja
Forgot, that the paraphyses are septate and have a vacuole at the tip.
Hans-Otto Baral,
23-06-2015 09:34
Re : Greyish on Rubus leaves
So Calycellina sp. Have no time to screen the literature. perhaps check Raitviir 2004?
Marja Pennanen,
23-06-2015 10:37
Re : Greyish on Rubus leaves
Calycellina populina and I have found it in the autumn of 2009 on Salix leaves...
Rubus should be taken as a broadleaved tree ;)
Thank you: Marja
Hans-Otto Baral,
23-06-2015 10:56
Re : Greyish on Rubus leaves
C. populina is certainly not. It has narrower spores with few oil. besides it is a Calycina today. You can test cresyl blue, it should stain the spore surface lilac in your sample but not in C. populina. I guess there is a gel around the spores in your fungus.
Marja Pennanen,
23-06-2015 13:55
Re : Greyish on Rubus leaves
Oops.
It was the one, that was at least near enough...
Cresyl blue is it the same as cotton blue?
What are the ingredients?
Marja
It was the one, that was at least near enough...
Cresyl blue is it the same as cotton blue?
What are the ingredients?
Marja
Hans-Otto Baral,
23-06-2015 14:32
Re : Greyish on Rubus leaves
No. cotton blue is usually in lactic acid or lactophenol and kills cells imediately. It does not at all give the stain here requited. Cotton blue in water is rarely used but I remember it does not make the metachromatic colour change although it stains gel. Cresyl blue in water changes from blue to lilac when staining gel.