05-06-2026 11:02
Thomas Læssøehttps://svampe.databasen.org/observations/10596691
07-06-2026 15:10
William Slosse
Hello everyone,On 05-06-26, I found following asco
07-06-2026 12:43
Steve ClementsBojour. This was a strange find on a stick on my
07-06-2026 12:09
François Freléchoux
Bonjour, Voici une brève description de ce qui m
12-07-2015 00:05
Nedim Jukic
This one from the same locality as the previous on
06-06-2026 17:44
Steve ClementsBonjour, This disco was on planed wood 3 x 1.5 cm
14-08-2016 23:15
Alex Akulov
Dear friendsCan you help me to find the descriptio
04-06-2026 11:36
Gernot FriebesHi,found on Vaccinium myrtillus.Asci: IKI –, 8-s
Demande
René Dougoud,
27-05-2015 09:48
J'ai trouvé dans Mycologia 71, 1979, un article de R.J. Bandoni: Safranin O as a rapid nuclear stain for fungi. (chez Cyberliber)
Ma demande : Avez-vous de l'expérience avec ce colorant nucléaire ?
Merci de vos réponses
René
Carmel Sammut,
28-05-2015 18:41
Re : Demande
Hi René,
I don't know if this is of any help. I have tried using o-safranin as a counterstain with toluidine blue. It didn't work but I use this mix for thin structure like paraphyses (in the attached photo from Propolis farinosa). It tends to overstain quickly.
I tried simple staining with o-safranin alone but I am not sure what I should see. I rarely use acetocarmine stains as I get mixed results with precipitates and unclean slides. I am attaching some tests on a Tarzetta - cupularis or gaillardiana (some spores are in the 26-27µm range). The stain was a 1% water solution added near the coverslip.
I don't know if this is of any help. I have tried using o-safranin as a counterstain with toluidine blue. It didn't work but I use this mix for thin structure like paraphyses (in the attached photo from Propolis farinosa). It tends to overstain quickly.
I tried simple staining with o-safranin alone but I am not sure what I should see. I rarely use acetocarmine stains as I get mixed results with precipitates and unclean slides. I am attaching some tests on a Tarzetta - cupularis or gaillardiana (some spores are in the 26-27µm range). The stain was a 1% water solution added near the coverslip.
