27-04-2026 20:52
Lothar Krieglsteiner
Found on hanging tiwg of Olea europaea in dried-ou
27-04-2026 18:48
Tony MoverleyCollected 23rd April 2026, Norfolk, EnglandSwarms
27-04-2026 17:41
Lothar Krieglsteiner
.. Algarve, same leaf than the last post. The con
27-04-2026 18:05
Lothar Krieglsteiner
... still attached at standing tree. The green con
27-04-2026 17:16
Lothar Krieglsteiner
.. Algarve, moist lying.The conidiomata look like
27-04-2026 12:54
Steve ClementsBonjour. Ce petit champignon blanc résupiné et
27-04-2026 09:59
Pauline. PennaBonjour Can anyone advise me on these pycnidia fo
22-04-2026 20:54
Enrique Rubio
Hi to everybody.This Pyrenopeziza grew in moist le
Hello everyone,
I hope anyone of you can give me a hand with this problem
On a pine cone I found a pair of yellow / orange slices. I'm not quite sure but think that there are only fours pores in the asci. The measure of the asci is between 75-85 x18 µm. The measure of the spores is between 23-26 x 4-7 µm. I thought there were konidiensporen but were appendages of the spores. Parafysen are knobby, J-.
Is there anyone who has an idea in which direction I should think?
Greetings
Gannie
If the asci are IKI+, it might be Pezicula eucrita. It grows right now, after the rains in mid-europe.
Cheers,
Guy
Hello Guy,
Thank you, I looked for some pictures and I think you're right. Can you explain what IKI+ and how to see this?
Greetings
Hannie
IKI is the so called Lugol´s solution (or "J" - but without chloral hydrate).
With such IKI, your fungus will show a hemiamyloid (= red!) reaction of the AA (apical apparatus).
In Melzer´s reagent, the reaction is negative.
Pre-treated with KOH (5%), the reaction will be blue in Melzer´s and in Lugol´s solution and not turning into red after a little while..
Cheers,
Guy
Hello Guy,
thanks for explanation. I did it with Lugol but I can't see any reaction.
Greetings
Hannie
Hello Guy,
yes it's pale yellow. It's hard to get it here in Holland, so someone sent it to me. Maybe he put some water to it? So it's better to buy some new?
Hannie
to my experience there are several varieties of Lugol's solution in trade. I once got one that was almost clear and prepared for lichenologists, since the reaction in lichen is very strong, but gives no sufficiant results with "standard" ascomycetes. On the other hand I experienced false descriptions of the reaction where concentrations of iodine stronger than 2% were used and thus an hemiamyloid reaction was suppressed or overrun.
The standard formula for Lugol's for ascomycetes should be 1% iodine + 3% potassium iodide added to water.
Regards
Martin
I have crystalline iodine and potassium iodide and mix it needed.
See http://www.invivoveritas.de/articles/iodine-reaction/
Zotto
Martin and Zotto,
thanks for the answer. I'll study your paper Zotto but it will take some time for me.
Cheers
Hannie