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Hello.A very tiny pyrenomycete sprouting sparsely
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ITS sequences from the following two collections B
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Blasco Rafael
Hola, en el mismo habitat que la anteriorRetamaDia
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Blasco Rafael
Hola, He recogido esta muestra de Orbilia sobre Re
Seed
Dragiša Savic,
22-04-2014 13:41
Hans-Otto Baral,
22-04-2014 16:12
Re : Seed
This should be the anamorph of Symphyosirinia clematidis on Clematis seed. Could you please take a photo of the conidia in water, under larger scale?
Dragiša Savic,
22-04-2014 21:14
Re : Seed
Bravo Zotto,
I'm sorry but this is not possible, I found it a month ago. I do not have my microscope and occasionally use one at the University. Therefore I use Lactofenol than water because it evaporates quickly.
I'm sorry but this is not possible, I found it a month ago. I do not have my microscope and occasionally use one at the University. Therefore I use Lactofenol than water because it evaporates quickly.
Hans-Otto Baral,
22-04-2014 21:23
Re : Seed
Evaporation of water is a problem but is slow enough to make 100 microphotos of a preparation.
It is well possible that the conidia are still alive in your dried specimen. If so and mounted in water they will contain lots of conspicuous drops. I put two bad photos here, one in water, the other in Cresyl blue.
Also a photo with apothecia and anamorph.
Zotto
It is well possible that the conidia are still alive in your dried specimen. If so and mounted in water they will contain lots of conspicuous drops. I put two bad photos here, one in water, the other in Cresyl blue.
Also a photo with apothecia and anamorph.
Zotto
Symphyosirinia-clematidis-HB-3938-2-0001.JPG
Symphyosirinia-clematidis-19.11.89-1-0001.jpg
Dragiša Savic,
22-04-2014 21:35
Re : Seed
No,no, I do preparation immediately and micrioscope later, sometimes after 10 to 20 days.
Hans-Otto Baral,
22-04-2014 21:47
Re : Seed
Let me please tell you that you should reconsider your methods. You loose a lot of information. The droplets in spores and conidia are only one of many features that are destroyed with your method. They are important species markers but are very difficult to use in dead material. When mounting in lactophenol they become even invisible and/or confluent.
Zotto
Zotto
Dragiša Savic,
22-04-2014 22:19
Re : Seed
Thanks, I'm still learning. I plan to buy a microscope soon.