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Bonjour !
Voici un Orbilia probable de la région de Québec.
Merci de toujours nous aider dans nos identifications.
Amitiés ! Roland
Données :
Date de récolte : 10 / 07 / 2-12
Substrat: feuillu pourri probable
Hyménium couvert d'une substance gélatineuse recouvrant les paraphyses et asques d'une coiffe foncée (épithécium ?)
Asques à 8 spores bisériées, cylindriques, atténués à la base, non amyloïdes ?,18,5-27 x 2,5-4 µm
Paraphyses cylindriques, 1,9 à 2,6 µm de diam.
Spores courtement filiformes à cylindriques, parfois légèrement arquées, hyalines, avec deux petites guttules polaires, 4,8-7 x 0,6-1 µm, 5,9 x 0,8 µm en moyenne, Q = 5,1=10, Q moyen = 7,6
Medulla ± celluleuse, formée de cellules subglobuleuses à cylindriques
Excipulum (ental ?) en textura porrecta, formé d'hyphes ventrues, à paroi épaissie jusqu'à 2,8 µm, septées, 23,5-68,5 x 13,5-33,5 µm
Excipulum ectal (latéral ?) en textura prismatica, formé d'hyphes septées, à paroi épaissie, courtes, 3,5-14 x 2-6 µm
Subiculum formé d'hyphes peu abondantes, subglobuleuses à la base, septées, à paroi épaissie jusqu'à 1,4 µm, 4,2-10,2 µm de diam.
Note : La petite taille de certains des éléments microscopiques et la présence d'un épithécium ont rendu les mesures et les observations difficiles à préciser.
this collection strongly resembles Hyalorbilia inflatula, the asci look like arising from croziers (Hyalorbilia) on Jacqeline's photostream. But perhaps Zotto has a different idea
best wishes,
Stip
Stip is right with Hyalorbilia, the asci have a rather wide, unstalked base and certainly arise from croziers, unlike Orbilia. Also the excipulum is of a horizontal prismatica.
But H. inflatula has straight spores (or almost so). We have H. tortuosa and H. fagi as the two species in question, both undescribed, and at least the latter quite frequent. But the two can not easily be separated. The best is when you find the conidia on the substrate (or in culture), they are quite different. From your narrow spore width H. tortuosa would better fit. Could you please send me spore photos with an included scale?
I attach a part of my key, so you can try whether you arrive at tortuosa.
The margin of your schematic section is not well figured, it needs to look round and a bit inrolled rather than cut. And the photo on the miidle left is misleading, it does not show a section but a squash mount. It pretends that the cells are oriented perpendicular to the surface while they run in fact parallel to the surface.
Zotto
Thanks you for the key.
If I consider that the spores are at most arcuate or sometimes subheliocoid, the key gives H. inflatula.
We will place new photo of spores.
Their dimensions are done with Piximètre.
Do you have ones that represent H. tortuosa, for comparaison.
We will make againt photos of excipulum and the margin.
We don't see conidia even when we observe the subicular hyphae on the substrat.
We will give you more informations. Please stay with us to go on.
Amitiés à vous et Stip !
Roland
Here a part of the H. inflatula plate where you see that the spores are generally straight or almost so.
Also plates of fagi and tortuosa in the documents below
Zotto
Thank you for the beautifull plates you place for us.
Now, we are sure that our Hyalorbilia is inflatula.
I change the micro plate of this species.
You will better see the spores and excipulum.
It'is a pleasure to work with you and with the teem of Ascofrance !
Merci et amitiés !
Jules, Roland and Jacqueline
your spores partly look like inflatula but others are too much curved. I never saw inflatula like that. I know it is a very difficult group, and perhaps intermediate cases exist. We have a few records we cannot safely place. Regrettably we have much too few sequences to get a better idea.
If you saw any conidia (~30-40 x 4-5 µm, multiseptate) in your collection this would be very helpful.
Could you please tell me the locality where it comes from?
Thanks
Zotto
Here is the locality : ville Lac-Beauport, comté de la Jacques-Cartier.
Finder : Jules Cimon
Herbarum : No 50466
Jules Cimon
5 chemin du Rouet Lac Beauport
G3B 0M3
Québec - Canada
Amitiés !
Roland
Zotto
OK, now I change the data for the locality, finder and herbarum.
If it is not enough, please ask again !
Roland
Lac Beauport lies at 46° 56' N 71° 58' W at ca. 250 m alt. Now, Jules Simon found it close to his home? And the number is his personal herbarium I think.
Zotto
Roland