Accès membres

Mot de passe perdu? S'inscrire

28-02-2026 15:52

Spooren Marco Spooren Marco

Who has an idea ? I have no coupes made for conid

27-02-2026 17:51

Michel Hairaud Michel Hairaud

Bonjour, Quelqu'un peut il me donner un conseil p

28-02-2026 14:43

Alain GARDIENNET Alain GARDIENNET

A new refrence desired :Svanidze, T.V. (1984) Novy

28-02-2026 11:54

Alain GARDIENNET Alain GARDIENNET

Hi forum,Is anyone aware if the 1936 edition of Si

28-02-2026 11:05

Yanick BOULANGER

Bonjour à tousLe 24/02/2026 à Montmacq, devant m

29-11-2024 21:47

Yanick BOULANGER

BonjourJ'avais un deuxième échantillon moins mat

27-02-2026 16:17

Mathias Hass Mathias Hass

Hi, Found this on Betula, rather fresh fallen twi

27-02-2026 12:56

Åge Oterhals

Found on fallen cones of Pinus sylvestris in midle

27-02-2026 11:21

Yannick Mourgues Yannick Mourgues

Hi to all. Here is a specie that can may be relat

26-02-2026 22:06

Malcolm  Greaves Malcolm Greaves

Can someone explain the features that split Geoscy

« < 1 2 3 4 5 > »
Hysteropezizella diminuens
Nina Filippova, 12-02-2013 16:44
I saw very beautiful blue coloring of VBs at tips of paraphyses  in Zotto's collection of this species, 9413 (CRB), which i did not succeed to see. Is it vital reaction with cresil blue?

From dead leaves of Eriophorum angustifolium, wet bog hollow.

Apothecia immersed in tissue, ellipsoid, to 350 x 170 mk, opening by ellipsoid slit, disc pale, surrounded by dark brown edge.

Excipulum from textura angularis at base, cells dark brown, about 10 mk in diam, textura prismatica at the edge, end cells (hairs) cylindrical or clavate, 15-20 x 5-8, brown, walls of end cells under immersion with roughness; asci clavate, inamyloid, 59-67 x 12,5-14,6; paraphyses cylindrical with ampulliform end cell, about 3 mk broad and segmented in lower part, ampulliform cell up to 5 mk in broadest part, hyaline, with small guttules (rarely seen in rehydrated), not or some exeeding the asci; spores subfusoid, many-guttulate, 17 (15,7-17,7) x 3,7 (3,4-4,2) (Q=4,67; N=13).
  • message #21842
  • message #21842
  • message #21842
  • message #21842
  • message #21842
  • message #21842
Hans-Otto Baral, 12-02-2013 18:09
Hans-Otto Baral
Re : Hysteropezizella diminuens
Yes, these are globose, rather inconspicuous (low-refractive) VBs which stain turquoise-blue in Cresylblue, but you see them only in living state.

Did you pretread your asci with KOH?

Hysteronaevia olivacea would have inamyloid asci, and paraph. as yours, but has much broader spores with acute ends.

Zotto

Nina Filippova, 12-02-2013 19:04
Re : Hysteropezizella diminuens
I used water-lugol, and MLZ and they were inamyloid, now added KOH, heated, and strong amyloid reaction happened. So, this shows they are actually amyloid?

Paraphyses hyaline and brownish in different fbs at one leaf, is seems variable.

I posted Hysteronaevia olivacea some before: http://www.ascofrance.fr/forum?page=1&id=21842
  • message #21851
Hans-Otto Baral, 12-02-2013 21:19
Hans-Otto Baral
Re : Hysteropezizella diminuens
This is the very typical hemiamyloid reaction, and it shows that your Lugol is insufficient. You should get a bright red reaction in water + lugol. Maybe your solution is almost colourless (the photo looks so), or it contains unallowed  ingredients. Hemiamyloid rings in Melzer are negative without KOH.

Sorry, I confused your two postings. So yes, this is H. diminuens. My answer on paraphyses, of course, concerned the Hysteronaevia, I will change it accordingly.

Zotto
Nina Filippova, 13-02-2013 11:55
Re : Hysteropezizella diminuens
There is ascus pore in fresh prepared lugol, it is still not pure red, apparently since J crystals not fully dissolved yet and solution is some weaker. But finally, i can see red reaction too.
  • message #21867
Hans-Otto Baral, 13-02-2013 12:09
Hans-Otto Baral
Re : Hysteropezizella diminuens
Yes, this is fine! Possibly the reaction has a blue component that becomes visible at lower iodine concentrations, resulting in a more dirty red (type RB).