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18-06-2019 16:55

Chris Yeates Chris Yeates

Bonjour tous a recent collection of damp leaves o

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Guy Buddy

Pennsylvania, USASpore measure from ejected spores

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Malcolm  Greaves Malcolm Greaves

A group of Scutellinia on a moss covered fallen br

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John Plischke John Plischke

2 weeks ago in New York we saw what appears to be

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Alessandro Fellin

Ritrovamento effettuato su tronco semisommerso e i

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Nicolas VAN VOOREN Nicolas VAN VOOREN

On dead stem of Veratrum, numerous dot-like specim

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Valencia Lopez Francisco Javier

Hola a todos/asAdjunto fotos de unas Geoporas reco

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Thomas Læssøe

In association with Nemania serpens (mostly effete

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Nicolas VAN VOOREN Nicolas VAN VOOREN

Hello.Here is a Hymenoscyphus species growing on b

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Riet van Oosten Riet van Oosten

Hello, Found by Theo Westra, 13-06-2019 (The Neth

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Paraphyses IKI reaction in Scutellinia
Perz Piotr, 03-02-2007 23:10
Hi,

I have question about paraphyses IKI reaction in genus Scutellinia. Sometimes paraphyses stain in IKI green - it means they have carotenoids. Sometimes there is no IKI reaction - also paraphyses does not contains carotenoids.

How is the name for violet-lila reaction? Here paraphyses of Scutellinia pseudotrechispora (Schröt.) Le Gal in IKI

  • message #1406
NC NC, 04-02-2007 10:10
Re:Paraphyses IKI reaction in Scutellinia
Bonjour,
J'ai déjà observé cette réaction violette avec le Lugol par exemple avec Scutellinia crucipila. Je pense que la réaction (carotene à l'extrémité des paraphyses)en vert foncé (vert-bouteille) s'arrete par manque de concentration en iode (??) et le mélange orange + vert foncé donne cette couleur lilas. Dans ton cas une préparation sans eau, seulement avec IKI donne t-elle toujours du violet?
Amicalement
Robert
Hans-Otto Baral, 04-02-2007 11:54
Hans-Otto Baral
Re:Paraphyses IKI reaction in Scutellinia
I have actually observed the same in "Scutellinia sp." (see my paper on Lugol versus Melzer, mycotaxon 29: 428, 1987): when adding IKI (Lugol) to a water mount, the colour reaction of the paraphyses changes from greyish-violet to grey.olive or grey-brown. The violet reaction is seen at an iodine concentration of 0.05-0.1% while the olive appears at 0.1-0.2 % and higher. So the colour is concentration-dependent similar as in the hemiamyloid apical rings. But contrary to hemiamyloidity, the violet and olive are also seen when KOH-pretreated, and I believe also in Melzer.

Zotto
Perz Piotr, 11-02-2007 09:00
Re:Paraphyses IKI reaction in Scutellinia
Thanks a lot, Robert & Zotto!