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Leandro Sánchez Leandro Sánchez

Sur branche de feuillus (Salix au côté)Spores 9,

27-07-2015 21:32

Dartanha Soares Dartanha Soares

Hi,Found this fungus on decorticated angiosperm wo

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Zuzana Sochorová (Egertová) Zuzana Sochorová (Egertová)

Hello,I found this Genea in Abkhazia (Great Caucas

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Michel RIMBAUD

Bonjour,J'ai récolté ce petit asco sur tige mort

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Leandro Sánchez Leandro Sánchez

Sur tige d'ombellifèreAsques 33-38/4-5, croziers

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Steve Clements

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Rubén Martínez-Gil Rubén Martínez-Gil

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Matthias Theiss

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Lepista Zacarias

2015-07-18 Pyreno on leaves Hi everyone, Maybe s

25-07-2015 07:55

Maren Kamke Maren Kamke

Hi everybody,I thought of M. typhicola, but the as

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Paraphyses IKI reaction in Scutellinia
Perz Piotr, 03-02-2007 23:10
Hi,

I have question about paraphyses IKI reaction in genus Scutellinia. Sometimes paraphyses stain in IKI green - it means they have carotenoids. Sometimes there is no IKI reaction - also paraphyses does not contains carotenoids.

How is the name for violet-lila reaction? Here paraphyses of Scutellinia pseudotrechispora (Schröt.) Le Gal in IKI

  • message #1406
NC NC, 04-02-2007 10:10
Re:Paraphyses IKI reaction in Scutellinia
Bonjour,
J'ai déjà observé cette réaction violette avec le Lugol par exemple avec Scutellinia crucipila. Je pense que la réaction (carotene à l'extrémité des paraphyses)en vert foncé (vert-bouteille) s'arrete par manque de concentration en iode (??) et le mélange orange + vert foncé donne cette couleur lilas. Dans ton cas une préparation sans eau, seulement avec IKI donne t-elle toujours du violet?
Amicalement
Robert
Hans-Otto Baral, 04-02-2007 11:54
Hans-Otto Baral
Re:Paraphyses IKI reaction in Scutellinia
I have actually observed the same in "Scutellinia sp." (see my paper on Lugol versus Melzer, mycotaxon 29: 428, 1987): when adding IKI (Lugol) to a water mount, the colour reaction of the paraphyses changes from greyish-violet to grey.olive or grey-brown. The violet reaction is seen at an iodine concentration of 0.05-0.1% while the olive appears at 0.1-0.2 % and higher. So the colour is concentration-dependent similar as in the hemiamyloid apical rings. But contrary to hemiamyloidity, the violet and olive are also seen when KOH-pretreated, and I believe also in Melzer.

Zotto
Perz Piotr, 11-02-2007 09:00
Re:Paraphyses IKI reaction in Scutellinia
Thanks a lot, Robert & Zotto!