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Adam Polhorský

Hello, I'm looking for description of Apodospora b

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Hi all, Found by a friend last Saturday in Staten

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Yanick BOULANGER

BonsoirJe pense que c'est Mollisia cinereaJ'ai hé

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Harald Homa Harald Homa

Hello everyone! While working through the finds f

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Angel Pintos Angel Pintos

Hello,anybody has the following article:Botryospha

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Vasileios Kaounas Vasileios Kaounas

found in the soil, in olive trees and Pistacia ver

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Blasco Rafael Blasco Rafael

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Hello,today my girlfriend Sylvie found a single ap

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Hi! Found this one on or next to some Juncus speci

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Paraphyses IKI reaction in Scutellinia
Perz Piotr, 03-02-2007 23:10
Hi,

I have question about paraphyses IKI reaction in genus Scutellinia. Sometimes paraphyses stain in IKI green - it means they have carotenoids. Sometimes there is no IKI reaction - also paraphyses does not contains carotenoids.

How is the name for violet-lila reaction? Here paraphyses of Scutellinia pseudotrechispora (Schröt.) Le Gal in IKI

  • message #1406
NC NC, 04-02-2007 10:10
Re:Paraphyses IKI reaction in Scutellinia
Bonjour,
J'ai déjà observé cette réaction violette avec le Lugol par exemple avec Scutellinia crucipila. Je pense que la réaction (carotene à l'extrémité des paraphyses)en vert foncé (vert-bouteille) s'arrete par manque de concentration en iode (??) et le mélange orange + vert foncé donne cette couleur lilas. Dans ton cas une préparation sans eau, seulement avec IKI donne t-elle toujours du violet?
Amicalement
Robert
Hans-Otto Baral, 04-02-2007 11:54
Hans-Otto Baral
Re:Paraphyses IKI reaction in Scutellinia
I have actually observed the same in "Scutellinia sp." (see my paper on Lugol versus Melzer, mycotaxon 29: 428, 1987): when adding IKI (Lugol) to a water mount, the colour reaction of the paraphyses changes from greyish-violet to grey.olive or grey-brown. The violet reaction is seen at an iodine concentration of 0.05-0.1% while the olive appears at 0.1-0.2 % and higher. So the colour is concentration-dependent similar as in the hemiamyloid apical rings. But contrary to hemiamyloidity, the violet and olive are also seen when KOH-pretreated, and I believe also in Melzer.

Zotto
Perz Piotr, 11-02-2007 09:00
Re:Paraphyses IKI reaction in Scutellinia
Thanks a lot, Robert & Zotto!