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Josep Torres Josep Torres

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Pourriez-vous me confirmer ma détermination de ce

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Dasyscyphella acutipilosa?
Miguel Ángel Ribes, 25-11-2010 00:54
Miguel Ãngel RibesBuenas noches

Esta vez con un verdadero ascomycete de menos de 0,5 mm de diámetro, ligeramente estipitado, sobre tallos herbáceos no identificados, con el himenio amarillo y el exterior cubierto de pelos blancos. Ascas cilíndrico-claviformes, acuminadas, biseriadas, con croziers y ápice azul con IKI. Esporas cilíndrico-fusiformes, muy estrechas y con un septo central en la madurez. Paráfisis lanceoladas, septadas, con pigmento amarillo en el ápice y el resto con aspecto marmóreo, ligeramente excedentes. Pelos finos, multiseptados, rectos, atenuándose hacia el ápice, lisos y con grandes cristales distribuidos de forma irregular, sobre todo en la parte basal. Excípulo ectal angular.
___________________________________________________
Good night

Now with a real ascomycete less than 0.5 mm, slightly stipitate, on unidentified herbaceous stems, with yellow hymenium and covered with white hairs. Asci cylindrical-clavate, acuminate, biseriate, with croziers and blue apex with IKI. Spores cylindrical-fusiform, very narrow, with a central septum at maturity. Paraphyses lanceolate, septate, with yellow pigment in the apex and the rest with marbling, slightly protruding. Fine hairs, multiseptate, straight, fading towards the apex, smooth and with large and irregularly distributed crystals, especially in the heel. Ectal and medular excipulum angular.

Spores measurement:
(11.3) 12.5 - 18.1 (19.1) x (1.7) 1.9 - 2.6 (2.8) µm
Q = (4.7) 5.4 - 8 (9.3) ; N = 66
Me = 15 x 2.3 µm ; Qe = 6.7

Asci measurement:
(59.2) 60.1 - 73.5 (83.2) x (4.3) 4.9 - 6.2 (6.8) µm
N = 35; Me = 67.5 x 5.5 µm

Thank you,

Miguel Ángel
  • message #13483
Miguel Ángel Ribes, 25-11-2010 00:54
Miguel Ãngel Ribes
Re:Dasyscyphella acutipilosa?
Another macro
  • message #13484
Miguel Ángel Ribes, 25-11-2010 00:55
Miguel Ãngel Ribes
Re:Dasyscyphella acutipilosa?
Asci acuminate with croziers
  • message #13485
Miguel Ángel Ribes, 25-11-2010 00:56
Miguel Ãngel Ribes
Re:Dasyscyphella acutipilosa?
Asci apex blue with IKI
  • message #13486
Miguel Ángel Ribes, 25-11-2010 00:57
Miguel Ãngel Ribes
Re:Dasyscyphella acutipilosa?
Spores with one septum
  • message #13487
Miguel Ángel Ribes, 25-11-2010 00:58
Miguel Ãngel Ribes
Re:Dasyscyphella acutipilosa?
Paraphysis lanceolate, yellow apex and silighlty protruding
  • message #13488
Miguel Ángel Ribes, 25-11-2010 00:59
Miguel Ãngel Ribes
Re:Dasyscyphella acutipilosa?
Hairs atenuated at apex and with large crystals
  • message #13489
Miguel Ángel Ribes, 25-11-2010 00:59
Miguel Ãngel Ribes
Re:Dasyscyphella acutipilosa?
And finally ectal excipulum with angular texture.

Thank you,

Miguel Ángel
  • message #13490
Hans-Otto Baral, 25-11-2010 09:28
Hans-Otto Baral
Re:Dasyscyphella acutipilosa?
Hi Miguel
the genus Albotricha is confirmed by molecular work as different from Dasyscyphella concerning A. acutipila and A. albotestacea. So my idea of Dasyscyphella seems wrong.

A. acutipila is strictly graminicolous, as far as I see, and now it depends on what your substrate is. I think this can be found out whether mono- or dicotyledonous, from the arrangement of vascular bundles (similar as with wood), by breaking the stem and looking on the cross-break.

I actually do not know these Albotricha species very well, and some are certainly misplaced in the genus. In my Dasyscyphella key I have listed D. acutipilosa under absent croziers, so this seems to exclude that species.
Zotto
Hans-Otto Baral, 25-11-2010 14:09
Hans-Otto Baral
Re:Dasyscyphella acutipilosa?
Looked again at your images. With my key I clearly arive at Albotricha caduca. The substrate looks like narrow xerophytic monocot leaves being folded. A collection I have seen form the high Alps (Davos) was on Nardus. Maybe yours is Festuca or the like? Was it also from high altitude?

Zotto
Miguel Ángel Ribes, 25-11-2010 15:20
Miguel Ãngel Ribes
Re:Dasyscyphella acutipilosa?
Hi Zotto

I am not the collector of the species, I will ask those who collected it, together with previous on Echinospartum, more or less at the same place: the Pyrenees, Huesca, Spain, so relatively speaking, it is from "high altitude".

Is the Albotricha key in your DVD? I am not capable to find it.

I have found this key in the internet, but I do not understand the languaje, sorry:

https://docs.google.com/viewer?url=http://www.mycokey.com/MycokeyDK/DKkeysPDFs/Hyaloscyphaceae.pdf&pli=1

Thank you very much
Marja Pennanen, 25-11-2010 16:12
Re:Dasyscyphella acutipilosa?
Hello.

I'll try to translate the description of A. caduca:
Sp. 10-15 x 2,5-3,5 µm. Frl. stilkede; skive 0,5-0,8 mm bred, gullig til orange; rand hvid-
håret; sp. kølleformede, encellede; sække 55-75 x 5,5-7,2 µm; parafyser lancetformede,
3-4 µm brede, 20-30 µm udragende; hår 130-210 x 3,5 µm, tilspidsede, tyndvæggede,
flercellede, glatte eller med få, løse korn; på græsser

Spores 10-15x2,5-3,5 µm. Fruitbody stipate, cup 0,5-0,8 mm wide, yelloish to orange, margin white haired; spores clavate?, one celled, ascus 55-75x5,5-7,2, paraphyses lanceolate, 3-4 µm wide, 20-30 µm exeeding the asci, hairs 130-210x3,5 µm, pointed, thin-walled?, septate, smooth or with some, easily loosen granules; on grasses.

My danish is not very good, but knowing a little bit of swedish helps ;)

The snow has fallen here: Marja
Miguel Ángel Ribes, 25-11-2010 20:21
Miguel Ãngel Ribes
Re:Dasyscyphella acutipilosa?
Hello Marja

Thank you for the translation. I think it do not fits exactly: ¿¿"spores clavate and one celled"??

Miguel Ángel
Hans-Otto Baral, 25-11-2010 23:21
Hans-Otto Baral
Re:Dasyscyphella acutipilosa?
I have two collections identifi9ed as A. caduca, one leg. et det. A. Raitviir. The spores in that were 15 x 3 µm, obtuse-cylindrical, the asci were with croziers.

The description of the other is here ( a few macros and hair micro is on the DVD, HB 7570):

On culms of Nardus stricta. Davos, Albula-Pass. Ap. 0,5-1,3mm, subsessile or with distinct short stalk, white. Asci *55-71 x 8.5-10 or 77-83 x 8 µm, apex conical, IKI 2-3bb Calycina-type, 8sp., with croziers. Spores *13-18 x 2.4-3.3 µm, Ölm.0-1 (a few small LBs near each end), elongate-cylindr., old 1-septate, -23.5µ long, often germinating by hyphae. Paraph. medium to strongly lanceolate, partly very acute, c. 10-15 µm projecting beyond living asci, *3.8-5.3µ wide, no content. hairs 100-127 or -160 x 3.5-4.3 µm, 8-10-celled, lower part thick, quite thin-walled, irreg. roughly warted & with abund. hyaline resin, no crystals, apex long tapering, */2-2.8 µm wide, upper 2-4 cells smooth somewhat longer than mid cells. Ect.exc. t. glob.-prism. firm-walled (*).

My Dasyscyphella (not Albotricha) key is here:

Zotto
Miguel Ángel Ribes, 26-11-2010 01:05
Miguel Ãngel Ribes
Re:Dasyscyphella acutipilosa?
I think this description and your photos in de DVD fits better with my collection. Only the asci are slightly different: acuminate and less broad in my collection, but I have been reviewing my photos and perhaps the acuminate apex occurs only in the young and empty asci, and the conical apex occurs at maturity (see attached photo).

Paraphysis wide: (3) 3.2 - 5 (5.4) µm; Me = 4 µm
Paraphysis projecting beyond asci: (8) 8.3 - 14 (20.6) µm; Me = 11.2 µm
Hairs wide at the apex: (1.7) 1.9 - 3.1 (3.6) µm; Me = 2.3 µm

Thank you Zotto and Marja.

Miguel Ángel

  • message #13507
Hans-Otto Baral, 26-11-2010 10:34
Hans-Otto Baral
Re:Dasyscyphella acutipilosa?
I do not know with which images you compared your acuminate asci. But the differenced to your new photo is simply that there are two living asci in the lower part above the paraphyses, and these are not acuminate because of the internal turgor.

Zotto
Miguel Ángel Ribes, 26-11-2010 11:46
Miguel Ãngel Ribes
Re:Dasyscyphella acutipilosa?
Ok, very clear your observation, I was comparing dead asci with living asci. So asci wide is bigger than my measurement. Sorry for my uselessness :(, I'm still learning to differentiate living and dead ;).

Thank you again,

Miguel Ángel
Miguel Ángel Ribes, 02-12-2010 23:42
Miguel Ãngel Ribes
Re:Dasyscyphella acutipilosa?
Dear Zotto

Sorry for the delay to replying. Regarding the habitat of the species, we are not sure about the identification of the substrate, but a botanical specialist told us that it could be a Festuca, but without security.

Next time we will try to identify the plant species.

Thank you,

Miguel Ángel
Jorge Hernanz, 06-12-2010 16:25
Re:Dasyscyphella acutipilosa?
Bonjour à tous!
I'm one of the collectors and guilty of not knowing the substrate. The problem is that the substrate are plant debris brought by the wind and retained within the plant pillow. The remains could belong to a multitude of plants around the area. In following collections I'll try to compare the plant debris with the various flat substrate in the area...
Thanks a lot
Jorge