14-04-2026 05:32
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Pyrenomycete with yellow subiculum on Aralia
Ethan Crenson,
14-04-2026 05:32
Hi all,
A few weeks back a friend pointed out some yellow fuzz in small cavities in stems of Aralia (A. elata or A. spinosa). I saw some depauperate perithecia among the fuzz. So I put the stem in a moist chamber for a couple of weeks.
A pyrenomycete has since grown. There are some small necks, fully covered in the same yellow subiculum. The subiculum turns brown-orange in KOH. This seems to be a Dothidiomycete. But if I am wrong about that, it is a Sordariomycete with a very thick unitunicate ascus and a
Asci IKI-, 174-224 x 13.7-17µm.
Pores brown, muriform with mostly 3 transverse septa and 2 or more longitudinal septa. 13.3-20.5 x 9.7-11.5µm
Paraphyses narrow.
Any help would be appreciated.
Thanks,
Ethan
Jacques Fournier,
14-04-2026 10:42
Re : Pyrenomycete with yellow subiculum on Aralia
Hi Ethan, nice little thing!
as usual with pyrenomycetes, a first critical step is to assess whether asci are uni-or bitunicate.
I say critical because it is often not easy to clearly assess.
IKI is not diagnostic but Congo red or coloured inks may be useful to understand the ascus apex anatomy.Judging from your photos the apex is not thickened but appears to be topped by a ring-like structure that would possibly stain in Congo red, heated if necessary.
If positive, this reaction would strongly suggest Sordariomycetes rather than Dothideomycetes and I suggest you to look into Thyridium, there is a recent paper by Sugita & Tanaka doi: 10.3897/mycokeys.86.78989 which might helpful.
All my best,
Jacques
as usual with pyrenomycetes, a first critical step is to assess whether asci are uni-or bitunicate.
I say critical because it is often not easy to clearly assess.
IKI is not diagnostic but Congo red or coloured inks may be useful to understand the ascus apex anatomy.Judging from your photos the apex is not thickened but appears to be topped by a ring-like structure that would possibly stain in Congo red, heated if necessary.
If positive, this reaction would strongly suggest Sordariomycetes rather than Dothideomycetes and I suggest you to look into Thyridium, there is a recent paper by Sugita & Tanaka doi: 10.3897/mycokeys.86.78989 which might helpful.
All my best,
Jacques
Ethan Crenson,
14-04-2026 19:22
Re : Pyrenomycete with yellow subiculum on Aralia
Hi Jacques,
Thank you for your help. I had considered Thyridium at first before the ascus anatomy confused me.
I have a feeling you are right about Thyridium, but in order to gain some experience with the microscopy I tried Congo Red (1% aqueous solution) and then I tried it again after heating and long soak (again 1% aqueous). Results are in the photo below.
I didn't get a lot of contrast, but there is something there that I am comparing to the illustration from the illustration in the Kendrick book I have. And of course it seems to align with the image in the Sugita and Tanaka paper you suggested:
More to come once I have looked at the paper.
Ethan
