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17-02-2013 08:38

Alain GARDIENNET Alain GARDIENNET

Bonjour, J'ai trouvé ces acervules sur feuille d

21-12-2024 09:08

Castillo Joseba Castillo Joseba

Me mandan el material seco de Galicia,  recolecta

21-12-2024 11:14

Michel RIMBAUD

Hello,Does somebody could send me a key for Olla/U

21-12-2024 12:45

Marc Detollenaere Marc Detollenaere

Dear Forum,On naked wood of Fagus, I found some ha

17-12-2024 12:33

Lothar Krieglsteiner Lothar Krieglsteiner

this fluffy anamorph was repeatedly found on decid

20-12-2024 20:30

Bernard CLESSE Bernard CLESSE

Bonsoir à toutes et tous,Pourriez-vous m'aider à

19-12-2024 09:27

Castillo Joseba Castillo Joseba

Llevo mas de 6 meses que no puedo contactar con Re

20-12-2024 00:01

Francois Guay Francois Guay

Hi, I found this species on incubated Fir needles

20-12-2024 20:34

Enrique Rubio Enrique Rubio

Perhaps some of you can help me identify this smal

18-12-2024 10:05

ruiz Jose antonio

Buenos días a todos, un pequeño ascomiceto de un

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Ascocoryne cylichnium
B Shelbourne, 13-10-2024 12:27
B Shelbourne• Macro and habitat suggest Ascocoryne.
• The lack of condiomata with so many apothecia seems distinct to many other examples fruiting on smaller and less decayed pieces of wood at the moment.
• Spores, structure of the medullary, abundant druses, and less inflated paraphysis apices support section cylichnium.
• Consulting Baral's key (2000), it must be A. cylichnium var. cyclichnium, based on the croziers (and almost uninflated and longer paraphysis apical cells, habitat, and maybe the conidial LBs).

Habitat: On the top and sides of a large angiosperm trunk, possibly Fraxinus, sodden and well decayed, lying on the ground, damp and shady area near several streams, flooding in very wet weather, mixed deciduous woodland, Low Weald, early October, after lots of rain, close to flooding.

Associates: A variety of fungi (espc. basidios) have been seen fruiting from the trunk throughout the year, currently Galerina marginata noticeable.

Apothecia: < 1 x 2 cm, usually 3-10+ caespitose,10+ groups on the trunk, purple, mostly cupulate to more discoid, appearing sessile or with a short and thick stipe, superficial, opaque, shape and margin appear variable when larger and more mature, possibly linked to exposure, margin round to several distinct lobes to undulating and densely scalloped, usually narrowing to a darker edge, receptacle paler, more pinkish, with an uneven-fleshy appearance, seen as many whitish lumps on the surface under low magnification (possibly druses), disc darker, vinaceous, waxy-gelatinous appearance, +/- uneven, sometimes with wart-like islands, initially convex, becoming plano-convex, sometimes eventually concave and umbilical, a light tomentum visible all over under low magnification, tough-gelatinous texture when sliced, internally lower part appears more gelatinised with a wet appearance.

Asci: Cylindrical-clavate, croziers, sometimes with a downward basal protrusion, apex bluing, rings bb or rarely rb, form looks typical for Ascocoryne, 2-2.5 seriate when turgid, apex rounded when turgid, becoming more conical-subtruncate when flaccid, acute-rounded when immature and sometimes rings not present or inamyloid.


Spores: Ellipsoid-fusoid, ends rounded-acute, often slightly heteropolar with base more elongated, inequilateral to very slightly curved in profile view, rarely slightly s-shaped or alternately curved, lower spores seem longer, filled with many smallish LBs (no large LBs seen), OCI 4-5 when fresh and mature, apparently uninucleate, some mature spores in a dead ascus appear to have 3-5 septa, one free spore seen germinating after some hours in water, with an ellipsoid outgrowth from each pole.


Spores free in water or in mature vital asci: (21.2) 21.8-26.8 (27.7) × 5.0-6.1 (6.3) µm, Q = (3.9) 3.92 - 5.1 (5.2), n = 13, mean = 24.4 × 5.5 µm, Q mean = 4.5.


Paraphyses: Narrow filiform, not to very slightly inflated at apex, with many smallish VBs in upper part of apical cell, coalescing somewhat over time, lower part of hymenium looks pink and more vinaceous en masse, sometimes branching from the central septa.


Ectal: Text. primatica-globosa, gradual transition in shape from medullary, hyaline globosa at surface, underneath a layer of dense pigment, purplish, continues to the margin, chains of globose-obpyriform cells at the margin, several cells protruding, shortish hyaline hyphae extending from the surface, branching occasionally, some druses and many crystals on the surface.


Medullary: Text. intricata, hyaline but more pigmented toward subhymenium, low part with more narrow and gelatinised hyphae, in the upper part and close to subhymenium with broader and more dense hyphae, many druses (clusters) and crystals of varying sizes, individually approximately octahedral, some cells with a relatively large globose refractive body inside (SCB?).


Subhymenium: Pigmented, dense with paraphysis bases.


Excudate: Some hyphae appear pigmented but pigment also exuded, clumping in the excipulum and hymenium, strongly gelatinised hymenium (epithecium), pigment seems to dissolve somewhat in KOH and more slowly in water.


Anamorph: Seem to be related, conidiophores on the surface of the excipulum, like the protruding hyphae, (botryoid?) conidia growing in small bunches at septa or apices, on individual short thin stalks, conidia 2.3-2.8 µm diameter, hyaline, globose, with several small or one larger LB.

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Hans-Otto Baral, 14-10-2024 11:07
Hans-Otto Baral
Re : Ascocoryne cylichnium
Of course this is cylichnium.

Interesting is the anamorph. We never saw it as in your case. The globose conidia appear to differ a bit from those on the ascospores. In our culture we know such globose conidia, but in unclarified samples from cinifer wood. A. cylichnium has rarely an anamorph with small sporodochia wit verticillate conidiophores and ellispoid-ovate conidia.

I would be glad to receive the collection data.

Fraxinus is easily IDed by its ring-pored wood and isolated small late-year vessels.

DNA data are still few in cylichnium. More is available for the very similar A. urnalis (earlier called deuncinata by us because of the lacking croziers. A. urnalis is much more consistent in the ITS than cylichnium (ITS tree attached).
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B Shelbourne, 14-10-2024 12:32
B Shelbourne
Re : Ascocoryne cylichnium
Thank you. The identification of this species in the key does seem rather simple in the end, druses + croziers.

There is no evidence that these anamorphic structures are related, but I could also see no overt evidence of parasitism and the short hairs are present on all of the apothecia. I looked at a water mount that I left wet, and I found some more conidia growing in the same way but most of these are more elongated, cylindrical-obovoid.

Hans-Otto Baral, 14-10-2024 14:44
Hans-Otto Baral
Re : Ascocoryne cylichnium
Strange, this does not look the same as the globose conidia. But both may occur in a culture of Ascocoryne. Wilson repeatedly made such observations. Here two images of her unpublished thesis attached.
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