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05-07-2025 12:38

Åge Oterhals

I found this pyrenomycetous fungi in pine forest o

01-06-2025 09:37

Charles Aron Charles Aron

Hi All, I found this Octospora growing with liver

06-07-2025 19:36

Castillo Joseba Castillo Joseba

me mandan el material de Galicia (España) recolec

07-07-2025 19:22

David Chapados David Chapados

Hi,Does anyone know what could this anamorph be?ht

02-07-2025 18:45

Elisabeth Stöckli

Bonsoir,Sur feuilles d'Osmunda regalis (Saulaie),

04-07-2025 20:12

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Hello.A fungus growing on the surface of a trunk o

20-06-2025 08:33

Josep Torres Josep Torres

Hello.Small, blackish, mucronated surface grains s

28-06-2025 16:00

Josep Torres Josep Torres

Hello.A tiny fungus shaped like globose black grai

04-07-2025 12:43

Castillo Joseba Castillo Joseba

me mandan el material seco de Galicia (España) 

03-07-2025 18:40

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Mollisia sp.
B Shelbourne, 16-01-2024 12:19
B ShelbourneI've had a go at identifying the Mollisia that I found the chytrid parasitising.

I think it could be Mollisia lividofusca (Fr.) Gillet, 1882. Any feedback appreciated.

This species seems to be 'incorrectly' named Tapesia lividofusca in Index Fungorum (later publication).

Ascomata: Apothecial, discoid, <= ~2 mm diameter, blackish-grey exterior, margin concolourous and uneven, disc pale whitish-grey when dry and darker bluish-grey when hydrated, margin in-rolled even in maturity, sessile, starting more urceolate, becoming acetabuliform, eventually +/- plane, often flexuous to irregularly lobate in maturity, glittering/micaceous especially exterior.

Habitat: Observed twice in January, gregarious to clustered, on decayed wooden fence rails, unidentified deciduous wood, top (more exposed) and middle rails of fence, ~0.5 – 1.25 m from the ground, presumably xeric, top and bottom sides of rails, decorticated parts, often in depressions and crevices, next to a pond in mixed deciduous woodland, southern England.
Some snow settling between observations, no noticeable effect on ascomata that were collected after snow but free from snow when collected.

Associated: Some ascomata parasitised by intra-hymenial Chytridomycete sp., evidenced by many sporangia appearing like asci in the hymenium, significantly supressing the host asci, and some motile zoospores ejected in water mount (see http://www.ascofrance.com/forum/77885/some-very-swollen-asci-on-a-suspected-mollisia-sp).
Subiculum appears to be part of an algal and dematiaceous biofilm that is visible on substrate around many ascomata, small/young thalli of lichens and bryophytes close by, tiny orange ascomata from an Orbilia sp. (indistinct macromorphology) also on the middle rail, basidiomata of Stereum cf. hirsutum on other corticated rails.

Preparation: Three ascomata from the middle rail examined microscopically, the first ascoma was stored for several days (in a damp container) prior to examination, but the following two were collected later and examined within hours of collection, water was used to mount unless otherwise stated.

Note that hymenial characters have been confirmed in the unparasitized ascoma (2), because clearly distinguishing the parasite 'rhizoids' has not been possible.

IKI: Apical ring bb, standard reactions elsewhere.

KOH: Ectal excipulum more brownish-olivaceous in thin sections, maybe a similar reaction in subhymenium, no reactions from hymenium.

Hymenium: Ochraceous en masse, no epithecium/exudate or gelatinous matrix identified.

Paraphyses: Filiform, sometimes gradually widening toward apex, rarely slightly swollen at apex, apex rounded, almost-completely filled with large cylindrical yellowish-hyaline VBs, 3 - 4 septa towards base, terminal cell longer.

Asci: 8-spored, croziers +, apex rounded, maybe slightly more truncate apex in front view.
Vital – 2 - 3 seriate, single spore at apex, mildly clavate, pars sporifera ~30 - 40%,
63.2 - 77.7 × 6.2 - 7.5 µm, N = 8.
Dead – usually uniseriate, more cylindrical, pars sporifera ~70-90%, often opaquely full of tiny LBs,
45.6 - 66.3 × 4.1 - 5.6 µm, N = 8.

Spores: Cylindrical to more allantoid or fusiform, occasionally narrowly-clavate, occasionally narrow-end slightly curved, inequilateral in profile view, aseptate, nucleus often visible, occasionally a few tiny shadowy LBs towards poles, OCI 0 (- 1).
Vital spores measured in squash mount (shorter spores probably immature) –
(7.6) 8.7 - 12.9 (13.9) × (2.3) 2.4 - 2.9 (3.2) µm,
Q = (2.6) 3.2 - 4.9 (5.1), N = 32,
Me = 10.5 × 2.7 µm, Qe = 3.9.

Subhymenium: Loose brownish hyphae.

Medullary excipulum: Hyaline to brownish, textura porecta?

Ectal excipulum: Blackish, textura globosa-angularis, some thick-walled.

Marginal cells/hairs: Brownish, usually one cell protruding, apices pyriform with large globose VB, or more cylindrical-hyphal with several medium-size VBs.

Subiculum: Anchoring hyphae brownish to hyaline, hyphae thick-walled, septate, clear contents, lots of hyphae found in/on the substrate with algae.

Possible anamorph: Diplococcium-like, seems micronematous, conidia - brownish, clear contents, 1 - 2 septate, basal cell usually wider, slightly constricted at some septa, acropetal, catenate? (maybe chains of two found),
4.7 - 9.4 × 3.1 - 5.7 µm,
Q = 1.4 - 2.2, N = 7,
Me = 7.1 × 4.3 µm, Qe = 1.7.
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Ingo Wagner, 16-01-2024 13:12
Ingo Wagner
Re : Mollisia sp.
Hello!

I think you are right with M. lividofusca.

Greetings
Ingo
B Shelbourne, 16-01-2024 14:16
B Shelbourne
Re : Mollisia sp.
Thank you very much for looking, Ingo. I don't think I would have got so close without your wonderful key!
Ingo Wagner, 16-01-2024 16:05
Ingo Wagner
Re : Mollisia sp.
Unfortunately the key is still very buggy.
I will add pictures in the future, for example of the spores.
The genus Mollisia is quite difficult.