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Thomas Flammer

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Trichobolus
Margot en Geert Vullings, 06-09-2023 12:29
This Trichobolus found on rabbit dung.

The length of the setae points to T. zukalii, but the spore size is not quite right? 

Spores 8.7 x 7.4 µ with Bary bubbles.

Only fruit bodies full of spores were found, no asci.

Setae length on average 350µ

Identification Ellis&Ellis.

Is this T. zukalii?

Thanks in advance, Margot
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Hardware Tony, 08-09-2023 10:44
Hardware Tony
Re : Trichobolus
Hello Margot,
your description fits best with T. zukalii to known other species that I can find. The ascus is almost the whole fruit body itself containing all the spores ranging from 1000 > 4000 sometimes depending on age. The setae fits well. This could also be the reason why your spores are slightly smaller but I suggest within range. The odd thing about these species is that Paul Cannon (Kew) states that this and other close species do not have deBary bubbles or oil spots etc. Perhaps there is a easy explanation for this but I am not aware. see:

https://fungi.myspecies.info/all-fungi/trichobolus-zukalii

I assume the examination was made with water as meltzers seem to expand the size of the spores.
regards
Margot en Geert Vullings, 09-09-2023 19:19
Re : Trichobolus
Thanks Tony for the answer.
The measurements were carried out in water.
I read on this site that some varieties do have deBary bubbles, but I cannot find which species:
https://fungi.myspecies.info/all-fungi/trichobolus

Kind regards, Margot
Hardware Tony, 09-09-2023 20:26
Hardware Tony
Re : Trichobolus
I foumd this article that provides a key to Trichobolus plus others.  Hope this helps.
Tony

https://www.mycosphere.org/pdf/MC3_4_No13.pdf
Norbert Heine, 09-09-2023 22:10
Norbert Heine
Re : Trichobolus
Hello Margot and Tony,
I know Trichobolus zukalii from nearly 50 finds and T. sphaerosporus from more than 10! In my opinion the best way for separation is the spore quotient. It is in middle 1,19 for zukalii and 1,07 for sphaerosporus (Doveri, FFI, 2004).
Regards, Norbert
Margot en Geert Vullings, 10-09-2023 13:46
Re : Trichobolus
Thank you both.

Tony, the key also takes you to T. zukalii.

Norbert, our spore quotiënt is 1.1 x 1.2 ( foto 4) this also points most to T. zukalii? Have you seen the Bary bubbles during these 50 observations?

Kind regards, Margot
Michel Delpont, 10-09-2023 16:40
Michel Delpont
Re : Trichobolus
Hello Margot

Same opinion as Norbert; what did you prepare for?

Michel.
Margot en Geert Vullings, 10-09-2023 16:53
Re : Trichobolus
Sorry Michel, I don't understand what you mean by "what did you prepare for"?

Kind regards, Margot
Michel Delpont, 10-09-2023 17:57
Michel Delpont
Re : Trichobolus
Excuse me, what liquid (only water?) do you use to make your Trichobolus preparations. Some can cause artifacts and modify certain details (this about  spores and Bary's bubbles).

Michel.
Margot en Geert Vullings, 10-09-2023 19:28
Re : Trichobolus
Tony also asked the same in the first answer :) everything was viewed and measured in water.

Margot


Norbert Heine, 10-09-2023 20:01
Norbert Heine
Re : Trichobolus
"Have you seen the Bary bubbles during these 50 observations?"

Yes, I did. But only on dried material, which was re-moisturized. The bubbles were thick-walled, just like you show.

Regards, Norbert

PS. Also I notized: Spores 8,5-9 x 6-7,5 µm when unripe!
Margot en Geert Vullings, 11-09-2023 08:44
Re : Trichobolus
"Yes, I did. But only on dried material, which was re-moisturized. The bubbles were thick-walled, just like you show".

This could very well be the explanation, because this could also have happened in nature before I found it.

Thanks and kind regards,

Margot