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02-01-2026 17:43

MARICEL PATINO

Hi there, although I couldn't see the fruitbody, I

04-01-2026 17:45

Stephen Martin Mifsud Stephen Martin Mifsud

I was happy to find these orange asmocyetes which

02-01-2026 22:48

éric ROMERO éric ROMERO

Bonjour tous, Je profite de cette nouvelle demand

02-01-2026 19:35

William Slosse William Slosse

Good evening everyone,First of all, my best wishes

03-01-2026 13:08

Niek Schrier

Hi all,We found groups of perithecia on a Lecanora

03-01-2026 15:36

éric ROMERO éric ROMERO

Bonjour, Pouvez-vous me dire quel est le nom à p

29-12-2025 17:44

Isabelle Charissou

Bonjour,J'aimerais savoir si d'autres personnes au

01-01-2026 18:35

Spooren Marco Spooren Marco

Original loamy soil aside a artificial lake.The co

31-12-2025 19:27

Spooren Marco Spooren Marco

Collected from loamy soil, at waterside (completel

29-12-2025 17:51

Blasco Rafael Blasco Rafael

Hola, me pueden ayudar con esta muestra.Recogida s

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Black apothecia with greenish septate spores on Picea abies twig
Edvin Johannesen, 23-03-2023 12:36
Edvin JohannesenThese black, erumpent, initially closed apothecia were found in small groups on a thin twig of an old spruce tree in February, in a mountainous area in central-southern Norway. They eventually open and disclose a yellowish white hymenium. The spores are greenish, which can be seen even in reflected light. The largest, open apothecium measure 2 mm.
Asci are 4-spored, clavate with a very long, alomost hypha-like base, ca. 20 micr. across at their broadest. Paraphyses filiform, ca. 1 micr. thick.
Spores 3-septate, greenish at maturity, 25-30 x 9-10 micr. 
Asci stain blue in Lugol, somewhat stronger towards the apex. Asci also weakly blue in Lugol after pre-treatment with KOH and water rinsing. Spores possibly also somewhat amyloid, but masked by the initial green color of spore wall.
Are we in Ostropales? In the "vicinity" of Tryblidiopsis?  Opinions appreciated.  Thanks!
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Edvin Johannesen, 23-03-2023 12:37
Edvin Johannesen
Re : Black apothecia with greenish septate spores on Picea abies twig
Asci and spores in water.
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Edvin Johannesen, 23-03-2023 12:38
Edvin Johannesen
Re : Black apothecia with greenish septate spores on Picea abies twig
Asci and spores in Lugol (no pre-treatment).
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Edvin Johannesen, 23-03-2023 12:39
Edvin Johannesen
Re : Black apothecia with greenish septate spores on Picea abies twig
Ascus in Lugol after KOH pre-treatment/rinsing.
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Edvin Johannesen, 23-03-2023 12:40
Edvin Johannesen
Re : Black apothecia with greenish septate spores on Picea abies twig
Ascus and spores in water.  Phase contrast to show septation.
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Hans-Otto Baral, 23-03-2023 16:53
Hans-Otto Baral
Re : Black apothecia with greenish septate spores on Picea abies twig
This reminds me of Lahmia kunzei = Parkerella populi, which is known from POpulus bark with much smaller apos (0.2-0.3 mm), 8-spored inamyloid asci, and apparently thin-walled, allantoid, 3-septate spores of 18-37 x 4-5. Only 1 species is known on the genus.

I have it in Tympanidaceae, but this is tentative and no sequence is known to me.
Edvin Johannesen, 23-03-2023 17:04
Edvin Johannesen
Re : Black apothecia with greenish septate spores on Picea abies twig
Thanks! So I would say, despite similarities, quite different from Lahmia kuntzei. Jason K. has offered to sequence it and perhaps also try to cultivate it. Do the spores look dead, by the way?
Hans-Otto Baral, 23-03-2023 17:15
Hans-Otto Baral
Re : Black apothecia with greenish septate spores on Picea abies twig
The multiguttulate ones are alive.
Jason Karakehian, 23-03-2023 19:15
Jason Karakehian
Re : Black apothecia with greenish septate spores on Picea abies twig
We should tell Luis Quijada about this if you think Tympanidaceae. The caespitose apothecia seem to suggest that, but I have no experience with the group.
Edvin Johannesen, 23-03-2023 21:51
Edvin Johannesen
Re : Black apothecia with greenish septate spores on Picea abies twig
OK, thanks again. I have been in touch with Luis a couple of times before, so I will bring this to his attention.