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06-03-2026 17:51

William Slosse William Slosse

Good evening all,On March 4, 2026, I found the fol

05-03-2026 10:07

Hulda Caroline Holte

Hello, I found and collected this species growing

06-03-2026 09:41

Alain GARDIENNET Alain GARDIENNET

Hi forum, I'm now looking for another reference c

05-03-2026 19:29

William Slosse William Slosse

Good evening everyone,On March 4, 2026, I found th

05-03-2026 16:30

François Bartholomeeusen

Dear forum members, On the 2nd of February 2026,

19-02-2026 17:49

Salvador Emilio Jose

Hola buenas tardes!! Necesito ayuda para la ident

28-02-2026 11:05

Yanick BOULANGER

Bonjour à tousLe 24/02/2026 à Montmacq, devant m

03-03-2026 20:34

Miguel Ãngel Ribes Miguel Ángel Ribes

Good eveningThese small, amphora-shaped perithecia

28-02-2026 11:54

Alain GARDIENNET Alain GARDIENNET

Hi forum,Is anyone aware if the 1936 edition of Si

02-03-2026 22:07

Jorge Hernanz

Buenas noches!Entre musgos, bajo Pinus halepensis

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Black apothecia with greenish septate spores on Picea abies twig
Edvin Johannesen, 23-03-2023 12:36
Edvin JohannesenThese black, erumpent, initially closed apothecia were found in small groups on a thin twig of an old spruce tree in February, in a mountainous area in central-southern Norway. They eventually open and disclose a yellowish white hymenium. The spores are greenish, which can be seen even in reflected light. The largest, open apothecium measure 2 mm.
Asci are 4-spored, clavate with a very long, alomost hypha-like base, ca. 20 micr. across at their broadest. Paraphyses filiform, ca. 1 micr. thick.
Spores 3-septate, greenish at maturity, 25-30 x 9-10 micr. 
Asci stain blue in Lugol, somewhat stronger towards the apex. Asci also weakly blue in Lugol after pre-treatment with KOH and water rinsing. Spores possibly also somewhat amyloid, but masked by the initial green color of spore wall.
Are we in Ostropales? In the "vicinity" of Tryblidiopsis?  Opinions appreciated.  Thanks!
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Edvin Johannesen, 23-03-2023 12:37
Edvin Johannesen
Re : Black apothecia with greenish septate spores on Picea abies twig
Asci and spores in water.
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Edvin Johannesen, 23-03-2023 12:38
Edvin Johannesen
Re : Black apothecia with greenish septate spores on Picea abies twig
Asci and spores in Lugol (no pre-treatment).
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Edvin Johannesen, 23-03-2023 12:39
Edvin Johannesen
Re : Black apothecia with greenish septate spores on Picea abies twig
Ascus in Lugol after KOH pre-treatment/rinsing.
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Edvin Johannesen, 23-03-2023 12:40
Edvin Johannesen
Re : Black apothecia with greenish septate spores on Picea abies twig
Ascus and spores in water.  Phase contrast to show septation.
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Hans-Otto Baral, 23-03-2023 16:53
Hans-Otto Baral
Re : Black apothecia with greenish septate spores on Picea abies twig
This reminds me of Lahmia kunzei = Parkerella populi, which is known from POpulus bark with much smaller apos (0.2-0.3 mm), 8-spored inamyloid asci, and apparently thin-walled, allantoid, 3-septate spores of 18-37 x 4-5. Only 1 species is known on the genus.

I have it in Tympanidaceae, but this is tentative and no sequence is known to me.
Edvin Johannesen, 23-03-2023 17:04
Edvin Johannesen
Re : Black apothecia with greenish septate spores on Picea abies twig
Thanks! So I would say, despite similarities, quite different from Lahmia kuntzei. Jason K. has offered to sequence it and perhaps also try to cultivate it. Do the spores look dead, by the way?
Hans-Otto Baral, 23-03-2023 17:15
Hans-Otto Baral
Re : Black apothecia with greenish septate spores on Picea abies twig
The multiguttulate ones are alive.
Jason Karakehian, 23-03-2023 19:15
Jason Karakehian
Re : Black apothecia with greenish septate spores on Picea abies twig
We should tell Luis Quijada about this if you think Tympanidaceae. The caespitose apothecia seem to suggest that, but I have no experience with the group.
Edvin Johannesen, 23-03-2023 21:51
Edvin Johannesen
Re : Black apothecia with greenish septate spores on Picea abies twig
OK, thanks again. I have been in touch with Luis a couple of times before, so I will bring this to his attention.