21-11-2025 11:52
Jean-Luc RangerBonjour à tous, on voit toujours 2 espèces areni
21-11-2025 10:56
Christopher Engelhardt
Very small (~0,5 mm) white ascos, found yesterday
14-11-2025 16:26
Marian Jagers
Hello everyone, On dead wood of Cytisus scoparius
17-11-2025 21:46
Philippe PELLICIERBonjour,Récolté sur bois pourrissant de feuillu
20-11-2025 14:14
Mick PeerdemanFound on the leaves of 'Juglans regia' in the Neth
20-11-2025 13:07
Mick PeerdemanIn January i found these black markings on the dea
Lactophenol Cotton Blue
Malcolm Greaves,
28-08-2022 11:04
I use this for staining the warts on Scutellinia and also when heating spores to see if the outer surface loosens. I have just spotted that one bottle I have is LCB but another is just Cotton Blue in Lactic Acid with no mention of the Phenol. Does anyone know what component of the stain causes the loosening?Thanks
Mal
Viktorie Halasu,
28-08-2022 11:44
Re : Lactophenol Cotton Blue
Hi Mal,
I usually use small amount of LACB to not overstain the preparate. When I suspected a species with loosening perisp. but it didn't show it even after gentle heating, I added a drop of lactic acid and then it loosened all right (after more heating or without, I don't remember). Pure LA is very viscous and goes rather slowly into the solution, I usually have to lift the edge of the coverslip a bit to help it overflow the section. And S. mirabilis preparate was not heated at all, but I left it overnight and the perisp. loosened too. I don't have any exper. with phenol, though.
Have a nice sunday.
Viktorie
I usually use small amount of LACB to not overstain the preparate. When I suspected a species with loosening perisp. but it didn't show it even after gentle heating, I added a drop of lactic acid and then it loosened all right (after more heating or without, I don't remember). Pure LA is very viscous and goes rather slowly into the solution, I usually have to lift the edge of the coverslip a bit to help it overflow the section. And S. mirabilis preparate was not heated at all, but I left it overnight and the perisp. loosened too. I don't have any exper. with phenol, though.
Have a nice sunday.
Viktorie
Malcolm Greaves,
28-08-2022 22:36
Re : Lactophenol Cotton Blue
Thanks Viktorie