28-04-2026 20:07
Lothar Krieglsteiner
... on twig in the air at standing Ceratonia siliq
14-04-2026 05:32
Ethan CrensonHi all, A few weeks back a friend pointed out som
28-04-2026 20:33
Vitus SchäfftleinHello, I found Trochila ilicina on Ilex aquifoliu
30-04-2026 10:28
Rot BojanHello, by appearance I would say that I am dealing
27-04-2026 18:48
Tony MoverleyCollected 23rd April 2026, Norfolk, EnglandSwarms
27-04-2026 20:52
Lothar Krieglsteiner
Found on hanging tiwg of Olea europaea in dried-ou
28-04-2026 22:51
Bernard CLESSE
Bonsoir à toutes et tous,Pourriez-vous m'aider à
29-04-2026 08:01
Lothar Krieglsteiner
... on twig attached to small tree of Citrus auran
29-04-2026 10:44
Lothar Krieglsteiner
growing at moist, drying-out soil at the side of a
Lactophenol Cotton Blue
Malcolm Greaves,
28-08-2022 11:04
I use this for staining the warts on Scutellinia and also when heating spores to see if the outer surface loosens. I have just spotted that one bottle I have is LCB but another is just Cotton Blue in Lactic Acid with no mention of the Phenol. Does anyone know what component of the stain causes the loosening?Thanks
Mal
Viktorie Halasu,
28-08-2022 11:44
Re : Lactophenol Cotton Blue
Hi Mal,
I usually use small amount of LACB to not overstain the preparate. When I suspected a species with loosening perisp. but it didn't show it even after gentle heating, I added a drop of lactic acid and then it loosened all right (after more heating or without, I don't remember). Pure LA is very viscous and goes rather slowly into the solution, I usually have to lift the edge of the coverslip a bit to help it overflow the section. And S. mirabilis preparate was not heated at all, but I left it overnight and the perisp. loosened too. I don't have any exper. with phenol, though.
Have a nice sunday.
Viktorie
I usually use small amount of LACB to not overstain the preparate. When I suspected a species with loosening perisp. but it didn't show it even after gentle heating, I added a drop of lactic acid and then it loosened all right (after more heating or without, I don't remember). Pure LA is very viscous and goes rather slowly into the solution, I usually have to lift the edge of the coverslip a bit to help it overflow the section. And S. mirabilis preparate was not heated at all, but I left it overnight and the perisp. loosened too. I don't have any exper. with phenol, though.
Have a nice sunday.
Viktorie
Malcolm Greaves,
28-08-2022 22:36
Re : Lactophenol Cotton Blue
Thanks Viktorie