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Lactophenol Cotton Blue
Malcolm Greaves,
28-08-2022 11:04
Thanks
Mal
Viktorie Halasu,
28-08-2022 11:44
Re : Lactophenol Cotton Blue
Hi Mal,
I usually use small amount of LACB to not overstain the preparate. When I suspected a species with loosening perisp. but it didn't show it even after gentle heating, I added a drop of lactic acid and then it loosened all right (after more heating or without, I don't remember). Pure LA is very viscous and goes rather slowly into the solution, I usually have to lift the edge of the coverslip a bit to help it overflow the section. And S. mirabilis preparate was not heated at all, but I left it overnight and the perisp. loosened too. I don't have any exper. with phenol, though.
Have a nice sunday.
Viktorie
I usually use small amount of LACB to not overstain the preparate. When I suspected a species with loosening perisp. but it didn't show it even after gentle heating, I added a drop of lactic acid and then it loosened all right (after more heating or without, I don't remember). Pure LA is very viscous and goes rather slowly into the solution, I usually have to lift the edge of the coverslip a bit to help it overflow the section. And S. mirabilis preparate was not heated at all, but I left it overnight and the perisp. loosened too. I don't have any exper. with phenol, though.
Have a nice sunday.
Viktorie
Malcolm Greaves,
28-08-2022 22:36
Re : Lactophenol Cotton Blue
Thanks Viktorie