05-03-2026 10:07
Hulda Caroline HolteHello, I found and collected this species growing
05-03-2026 16:30
François BartholomeeusenDear forum members, On the 2nd of February 2026,
19-02-2026 17:49
Salvador Emilio JoseHola buenas tardes!! Necesito ayuda para la ident
03-03-2026 20:34
Miguel Ángel Ribes
Good eveningThese small, amphora-shaped perithecia
01-03-2026 18:02
Francois Guay
I found this mystery Helotiales on an incubated le
28-02-2026 14:43
Alain GARDIENNET
A new refrence desired :Svanidze, T.V. (1984) Novy
Lactophenol Cotton Blue
Malcolm Greaves,
28-08-2022 11:04
I use this for staining the warts on Scutellinia and also when heating spores to see if the outer surface loosens. I have just spotted that one bottle I have is LCB but another is just Cotton Blue in Lactic Acid with no mention of the Phenol. Does anyone know what component of the stain causes the loosening?Thanks
Mal
Viktorie Halasu,
28-08-2022 11:44
Re : Lactophenol Cotton Blue
Hi Mal,
I usually use small amount of LACB to not overstain the preparate. When I suspected a species with loosening perisp. but it didn't show it even after gentle heating, I added a drop of lactic acid and then it loosened all right (after more heating or without, I don't remember). Pure LA is very viscous and goes rather slowly into the solution, I usually have to lift the edge of the coverslip a bit to help it overflow the section. And S. mirabilis preparate was not heated at all, but I left it overnight and the perisp. loosened too. I don't have any exper. with phenol, though.
Have a nice sunday.
Viktorie
I usually use small amount of LACB to not overstain the preparate. When I suspected a species with loosening perisp. but it didn't show it even after gentle heating, I added a drop of lactic acid and then it loosened all right (after more heating or without, I don't remember). Pure LA is very viscous and goes rather slowly into the solution, I usually have to lift the edge of the coverslip a bit to help it overflow the section. And S. mirabilis preparate was not heated at all, but I left it overnight and the perisp. loosened too. I don't have any exper. with phenol, though.
Have a nice sunday.
Viktorie
Malcolm Greaves,
28-08-2022 22:36
Re : Lactophenol Cotton Blue
Thanks Viktorie