12-11-2019 10:32
Miguel Ángel Ribes
Hi againExactly at the same place than my previous
25-12-2019 17:54
Valencia Lopez Francisco JavierHola a todos/asEstas supuestas pezizas estaban en
28-07-2011 18:31
Alex Akulov
Dear FriendsToday I made the pdf file of Velenovsk
12-07-2015 00:05
Nedim Jukic
This one from the same locality as the previous on
30-05-2026 21:12
Philippe PELLICIERSur branche de mélèze (Larix) près de la neige,
31-05-2026 10:35
Hulda Caroline HolteHello,I collected this species growing on a rather
25-05-2026 16:35
Bernard CLESSE
Bonjour à toutes et tous,J'ai trouvé récemment,
29-05-2026 15:35
daniel FERREBonjour à tous,Je voudrais votre aide pour cette
Lactophenol Cotton Blue
Malcolm Greaves,
28-08-2022 11:04
I use this for staining the warts on Scutellinia and also when heating spores to see if the outer surface loosens. I have just spotted that one bottle I have is LCB but another is just Cotton Blue in Lactic Acid with no mention of the Phenol. Does anyone know what component of the stain causes the loosening?Thanks
Mal
Viktorie Halasu,
28-08-2022 11:44
Re : Lactophenol Cotton Blue
Hi Mal,
I usually use small amount of LACB to not overstain the preparate. When I suspected a species with loosening perisp. but it didn't show it even after gentle heating, I added a drop of lactic acid and then it loosened all right (after more heating or without, I don't remember). Pure LA is very viscous and goes rather slowly into the solution, I usually have to lift the edge of the coverslip a bit to help it overflow the section. And S. mirabilis preparate was not heated at all, but I left it overnight and the perisp. loosened too. I don't have any exper. with phenol, though.
Have a nice sunday.
Viktorie
I usually use small amount of LACB to not overstain the preparate. When I suspected a species with loosening perisp. but it didn't show it even after gentle heating, I added a drop of lactic acid and then it loosened all right (after more heating or without, I don't remember). Pure LA is very viscous and goes rather slowly into the solution, I usually have to lift the edge of the coverslip a bit to help it overflow the section. And S. mirabilis preparate was not heated at all, but I left it overnight and the perisp. loosened too. I don't have any exper. with phenol, though.
Have a nice sunday.
Viktorie
Malcolm Greaves,
28-08-2022 22:36
Re : Lactophenol Cotton Blue
Thanks Viktorie