Accès membres

Mot de passe perdu? S'inscrire

19-02-2026 13:50

Margot en Geert Vullings

We found this collection on deciduous wood on 7-2-

19-02-2026 12:01

Castillo Joseba Castillo Joseba

Me mandan el material de Galicia (España), recole

17-02-2026 09:41

Maren Kamke Maren Kamke

Good morning, I found a Diaporthe species on Samb

16-02-2026 21:25

Andreas Millinger Andreas Millinger

Good evening,failed to find an idea for this fungu

08-12-2025 17:37

Lothar Krieglsteiner Lothar Krieglsteiner

20.6.25, on branch of Abies infected and thickened

17-02-2026 17:26

Nicolas Suberbielle Nicolas Suberbielle

Bonjour à tous, Je recherche cette publication :

03-02-2013 19:50

Nina Filippova

Good time), I've compared this specimen with the

15-02-2026 04:32

Tomaz Vucko Tomaz Vucko

One more specimen that is giving me some descent a

17-02-2026 13:41

Isabelle Charissou

Bonjour, est-ce que quelqu'un pourrait me fournir

16-02-2026 18:34

Thierry Blondelle Thierry Blondelle

Bonjour,La micro de cet anamorphe de Hercospora su

« < 1 2 3 4 5 > »
Erumpent on Wattle Part 2
Zuidland Peter, 25-06-2022 07:08
Hello all,
I view of possible confusion by poor images I representwhat I think is an erumpent I have found on Wattle. I have used Spooner's Heliotales of australia for years and I find nothing to help me in there, there is no budding like this on Heliotales according to him.
This sample forms under the bark, breaks through as it gets bigger and spreads across the wood surface. One image shows old craters with remnant excipulm around the edges under the bark.
Fresh images from today, all microscopy in water; sample is being kept moist and cool for further work if needed.

SE Victoria Australia 
Cheers
Pete
  • message #73136
  • message #73136
  • message #73136
  • message #73136
  • message #73136
  • message #73136
  • message #73136
  • message #73136
Hans-Otto Baral, 25-06-2022 09:23
Hans-Otto Baral
Re : Erumpent on Wattle Part 2
Helpful would be a closeup of the marginal lobes in section (last pic), if there are periphysoids. It could be an ostropalean fungus. Of course, free spores would be good and their measurements. Did you test IKI? MLZ is unsuitable, unless you pretreat with KOH.
Zuidland Peter, 25-06-2022 09:51
Re : Erumpent on Wattle Part 2
Thanks heaps Hans Otto,

I did check for IKI with Lugol's and it is the first micro image in my previous post on this( see below again); I have two more samples that I can try to check what you have asked for. 

Take care

Pete
  • message #73138
Zuidland Peter, 26-06-2022 04:21
Re : Erumpent on Wattle Part 2
I think these are the periphysoids with arrows.

Pete
  • message #73150
  • message #73150
Zuidland Peter, 26-06-2022 04:24
Re : Erumpent on Wattle Part 2
Free spores in H2O measured: 16.4 - 20.3 x 10.2 - 13.5um

many thanks

Pete
  • message #73151
  • message #73151
Hans-Otto Baral, 26-06-2022 18:00
Hans-Otto Baral
Re : Erumpent on Wattle Part 2
O.k., I am now a bit confused, do you think your wattle 1 and 2 are the same species?

I saw the IKI-ascus photo in 1 and wondered if it is a hemiamyloid apical ring or only plasma. It could be a ring, and then you should either show it in larger magnification or try KOH-pretreatment to see if the ring then stains blue in IKI.

Periphysoids: I am not sure. They often form a compact layer of short parallel hyphae embedded in some gel.
Zuidland Peter, 27-06-2022 07:42
Re : Erumpent on Wattle Part 2
I am sorry that my poor skills as a non trained mycologist are causing you some grief or confusion, I don't mean to.
The specimen is the same in both series, I have no other asco near it nor with these spores.
Here is sample with just Lugol's again, from today and zoomed.

Pete
  • message #73160
  • message #73160
Zuidland Peter, 27-06-2022 07:45
Re : Erumpent on Wattle Part 2
Here is the sample heat treated with 4%KOH, cooled, remove most KOH apply Lugol's.

This leaves a clear looking asci with no stain. 

  • message #73162
  • message #73162
Zuidland Peter, 27-06-2022 07:47
Re : Erumpent on Wattle Part 2
Heat treat with KOH, cool, add Lugol's, remove Lugol's then add Lugols again.

This left the asci stained.
  • message #73163
  • message #73163
Zuidland Peter, 27-06-2022 07:49
Re : Erumpent on Wattle Part 2
For the excercise I did it with MLZ too.

Heat with KOH, remove then add MLZ

One image looks to show a channel near the tip
  • message #73164
  • message #73164
Hans-Otto Baral, 27-06-2022 08:21
Hans-Otto Baral
Re : Erumpent on Wattle Part 2
Splendid and much better resolution! Now it is clear, the asci are inamyloid. The "channel" is an ocular chamber, maybe it is more typical of immature asci without spores or with beginning spore formation. In such asci it my  be that the lateral ascus wall is thickened.