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Hyalorbilia erythrostigma help
Hardware Tony,
12-04-2022 15:44

Body: Pale yellow, translucent, slightly gelantinous, scattered with perhaps short stipe but unclear. 0.1 - 0.3mm, no hairs, paraphyses not seen but possible,
Ascospores: Not clear, but a few visible suggested at 2.3µm and subglobose showing with SBs -just.
Asci: 18 - 24 µm x 4/5, bluing capped exudate? in iodine.
Excipulum: in rows like 'brickwork' prismata. 12 - 20 µm wide x 4/5.
No conidia T-cells observed.
Apprecaite any help, with thanks
Jean-Paul Priou,
12-04-2022 20:17

Re : Hyalorbilia erythrostigma help
Clearly an Hyalorbilia,it seems to have more 8 spores in asci, difficult to count them !
It exists a pleioerythrostigma 16 spores from Australia !
JPP
Hardware Tony,
12-04-2022 21:22

Re : Hyalorbilia erythrostigma help
Thks Jeen-Paul, entirely possible as I was involved in finding a new species of Basidiomycetes last October in the UK called Cruentomycena visidocruenta - more or less only found in Australia/NZ and new for Europe and beyond! It does appear to have spores at the lower level within the ascus and in Otto's immense Monograph on Orbiliomycetes he states it is even close to the target speces above! However, it appears that there are no SBs in the ascospores of H. pleioerythrostigma and the description of the Ectal excipulum suggested globulosa-angularis content, not sure what that is mind you! Also the only 16 spored species so many thks for highlighting this.
With tks Tony
With tks Tony
Hans-Otto Baral,
12-04-2022 21:34

Re : Hyalorbilia erythrostigma help
A blue iodine reaction is certainly not the case. Maybe a refraction effect.
The asci appear to me immature, I wonder if it is only immature H. inflatula.
H. erythrostigma is never cupulate and smaller, is the large apo not larger than 0.3 mm?
Hardware Tony,
12-04-2022 23:32

Re : Hyalorbilia erythrostigma help
Thks Otto.
Must be refraction as observed in water as well.
However, still difficult to see as H. inflatula as the ascospores (what can be seen) do not appear thinly cylidrical at all, and what I have seen on the occasions of finding H. inflatula is that it is much larger, more translucent and flatter/sessile. Also I don't think this grows on stromata of other ascos?
The pic was taken through my scope at x40 mag, and so this was the 0.3mm sample, maybe very slightly bigger perhaps.
Accept if you don't think this is H. erythrostigma. Annoyed I couldn't get my trusty 1000x to be clearer at the time. Many thks Tony
Must be refraction as observed in water as well.
However, still difficult to see as H. inflatula as the ascospores (what can be seen) do not appear thinly cylidrical at all, and what I have seen on the occasions of finding H. inflatula is that it is much larger, more translucent and flatter/sessile. Also I don't think this grows on stromata of other ascos?
The pic was taken through my scope at x40 mag, and so this was the 0.3mm sample, maybe very slightly bigger perhaps.
Accept if you don't think this is H. erythrostigma. Annoyed I couldn't get my trusty 1000x to be clearer at the time. Many thks Tony
Hans-Otto Baral,
12-04-2022 23:55

Re : Hyalorbilia erythrostigma help
H. inflata actually often grew on other fungi, including stromata. Maybe I missed the detail where you see the spores.
Viktorie Halasu,
13-04-2022 08:40

Re : Hyalorbilia erythrostigma help
Hello,
I found some pictures of immature Hyalorbilia asci filled with small vacuoles, some seemingly have a "nucleus". Looks a bit similar to Tony's photos. You can see the mature asci with spores too.
Viktorie
I found some pictures of immature Hyalorbilia asci filled with small vacuoles, some seemingly have a "nucleus". Looks a bit similar to Tony's photos. You can see the mature asci with spores too.
Viktorie
Hardware Tony,
13-04-2022 09:38

Re : Hyalorbilia erythrostigma help
Thks Viktorie for your solution. This probably explains the odd spherical looking spore growth and put this all down to a very immature H. inflatula as Otto indicates. To many oddities for something rarer especially when I found it on two different substrates 500 meters apart both in the same state.
Thks again Tony
Thks again Tony