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Villalonga Paco

Small Scutellinia growing in garden soil (calcareo

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Who can help? Malloch, D.; Hubart, J.-M. 1987. An

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Bryoscyphus phascoides?
Georges Greiff, 10-03-2022 23:23
Bonsoir,

I hope everybody is well.

I wanted to check the ID of a Bryoscyphus, which appears to be B. phascoides, that I was sent recently. It was growing on lower parts of Rhytidiadelphus squarrosus moss shoots. Based on some searches and material in Zotto's folder, I think this is correct. Apparently, this fungus is rather common on R. squarrosus according to some other AscoFrance comments.

I have put a link to the page with images on my website / blog below. Please excuse the poor quality.


Thanks and best wishes,

George
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Hans-Otto Baral, 11-03-2022 09:56
Hans-Otto Baral
Re : Bryoscyphus phascoides?
Yes, to my memory this fits well.
Georges Greiff, 11-03-2022 12:42
Re : Bryoscyphus phascoides?
Thank you, Zotto.

I don't immediately see labelled sequence info on this on Genbank. I hope to get ITS and LSU for it as the collector has found more material. Along with recent B. rhombisporus and two Pithyella spp., I hope we can understand a bit more about the relationships between these genera.

Thanks again and best wishes,
George
Hans-Otto Baral, 11-03-2022 17:11
Hans-Otto Baral
Re : Bryoscyphus phascoides?
This would be great! Perhaps you can do another try for the ascus base?

There exists ITS and LSU for a sample by Ingo Wagner (not in GB) which has simple septa.
Georges Greiff, 11-03-2022 17:17
Re : Bryoscyphus phascoides?
Thanks Zotto. I will have a proper look at the ascus bases as I kept some tissue.

All the best,
George
Georges Greiff, 15-03-2022 00:36
Re : Bryoscyphus phascoides?
Hi Zotto,

I hope these photos help to clarify. I received more material with enough apothecia for sequencing, so hopefully that will work.

All the best,

George
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Hans-Otto Baral, 15-03-2022 08:16
Hans-Otto Baral
Re : Bryoscyphus phascoides?
Perfect! No croziers. I am curious for the DNA result!
Georges Greiff, 15-03-2022 10:59
Re : Bryoscyphus phascoides?
Thanks Zotto. Me too. Giving it a shot this coming weekend so fingers crossed they work.

Best wishes,
George
Patrice TANCHAUD, 15-01-2023 22:20
Re : Bryoscyphus phascoides?
Bonjour Georges,

as-tu un résultat ADN pour ta récolte ?
Merci.

Patrice
Georges Greiff, 16-01-2023 10:17
Re : Bryoscyphus phascoides?
Hi Patrice,

Unfortunately it did not work that time. I have the material so I will try ITS & LSU again when I can and let you know here.

All the best,
George
Patrice TANCHAUD, 16-01-2023 10:47
Re : Bryoscyphus phascoides?
Understood, thank you Georges. I asked you the question because I have a harvest that looks like yours, without hooks at the base of the asci.

All the best.

Patrice
Hans-Otto Baral, 16-01-2023 11:28
Hans-Otto Baral
Re : Bryoscyphus phascoides?
Yes this appears to be the same thing, and who knows which is the true phascoides. Could you please provide a bit of data such as date and host?
Patrice TANCHAUD, 16-01-2023 11:37
Re : Bryoscyphus phascoides?
Unidentified moss on the ground, in the town of St Georges de Didonne (Charente-Maritime), several harvests between January 1 and 15, 2023.

Patrice


Georges Greiff, 16-01-2023 12:13
Re : Bryoscyphus phascoides?
Thanks. Would you be able to show some different pictures of the moss host from further away? I might be able to identify it.

Best,
George
Michel Hairaud, 16-01-2023 16:15
Michel Hairaud
Re : Bryoscyphus phascoides?
Bonjour, 

I would also believe there might be several B. phascoides . At least the H- and H+ forms have clearly been documented. 
It would be interesting to check whether spore guttulation and spore shapes are the same for these both ''forms' I hereby join 2 pics showing spores of them.

Amitiés 
Michel
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Patrice TANCHAUD, 16-01-2023 16:20
Re : Bryoscyphus phascoides?
Voici.
Georges Greiff, 16-01-2023 16:22
Re : Bryoscyphus phascoides?
Thanks. The host seems to be Hypnum cupressiforme var. lacunosum.
Patrice TANCHAUD, 16-01-2023 18:11
Re : Bryoscyphus phascoides?
Merci Georges.
Hans-Otto Baral, 16-01-2023 19:00
Hans-Otto Baral
Re : Bryoscyphus phascoides?
I could not find a difference in spore size and guttulation between the two groups. Typical is that a larger LB is in the broader part and a smaller LB in the narrower part of the spore, each surrounded by small ones. But a tendency to more mutliguttulate is also observed as one to large-biguttulate. I think this depends also on spore maturity or external influence.

Your two samples, Michel, I might not have, at least the right one I did not see, not anything comparable to it.
Hans-Otto Baral, 21-01-2023 10:27
Hans-Otto Baral
Re : Bryoscyphus phascoides?
Hi Michel

I stumbled again at your two pics. You spoke about H+ and H- but you did not say which of the two photos are H+ and which H-. Do you not know?

Zotto
Michel Hairaud, 21-01-2023 15:01
Michel Hairaud
Re : Bryoscyphus phascoides?
Bonjour Zotto, 
Yes I think I should know -:) 
Here are pics of the H+ one. 
If data needed, will send next week, once back home

Amitiés
Michel
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Hans-Otto Baral, 22-01-2023 09:48
Hans-Otto Baral
Re : Bryoscyphus phascoides?
Yes, I would be interested in the data.