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Patellaria
Yannick Mourgues, 21-03-2009 22:19
Yannick MourguesBonsoir.

Voici un asco trouvé sur branche morte décortiquée de Cytisus scoparius.
Macro :
Ascome discoide +- cupulé. Hyménium gris-olivâtre frais, puis noir en séchant. Excipulum noir et lisse.
Micro:
Asques à priori bituniqués, 90-95x10-14 um, IKI-.
Spores hyalines avec 7 cloisons à maturité, une grosse gutulle dans chaque partie, 29,6-34,2x4,8-5,6 um Q=5,5-5,8
Aucune réaction dans KOH.

J'arrive à Patellaria atrata.
Confirmez-vous ?


Here is an asco found on dead decayed branch of Cytisus scoparius.
Macro: Ascome discoide + - cupulé. Hyménium grey - olive-greenish freshly, then black by drying. Black and smooth Excipulum.
Micro:
Asques in priori bitunicates, 90-95x10-14 um, IKI-.
Hyalines spores with 7 partitions with maturity, one big gutulle per party, 29,6-34,2x4,8-5,6 um Q=5,5-5,8
no reaction in KOH.

I arrive at Patellaria atrata.
What do you think about that ?

Yannick Mourgues
  • message #7184
Yannick Mourgues, 21-03-2009 22:20
Yannick Mourgues
Re:Patellaria
When dry ...

Une fois sec ...
  • message #7185
Yannick Mourgues, 21-03-2009 22:21
Yannick Mourgues
Re:Patellaria
Les spores ...
  • message #7186
Yannick Mourgues, 21-03-2009 22:21
Yannick Mourgues
Re:Patellaria
Asques dans Lugol

et spores bisériés ...
  • message #7187
Hans-Otto Baral, 21-03-2009 22:46
Hans-Otto Baral
Re:Patellaria
Hi Yannich

your asci look to me unitunicate. Surely not Patellaria. How are the paraphyses? In Patellaria they are branched at the top and immersed in dark olive or bluish exudate. Your Lugol is invisible, needs to dissolve some iodine crystals to get deep redbrown.

Apart from the iodine reaction a section showing the excipulum might help.
Zotto

Yannick Mourgues, 21-03-2009 23:23
Yannick Mourgues
Re:Patellaria
Hi Zotto.
Asci are unitunicate, yes. Paraphyses aren't like Patelalria's.
They are filiforms, septate, hyalines with gutulles.
I redo some photos of all this tomorrow.
Yannick
Yannick Mourgues, 22-03-2009 21:06
Yannick Mourgues
Re:Patellaria
Bonsoir.
Réaction IKI- confirmée.
Je n'ai pas pu faire une coupe car l'ensemble est trop gélatineux, trop mou. Mais à part cela, l'excipulum est formé de cellules cylindriques brunes, ne dépassant pas 10x4 um. Ensuite, entre l'excipulum et les asques, une couche de cellules hyalines rondes de diamètre 10-15um.
Asques unituniqués.

Une idée ?


Good evening.
Confirmed reaction IKI-.
I wasn't able to make a section because the it'is too gelatinous, too soft. But beyond that, the excipulum is formed by brown cylindrical cells, not overtaking 10x4 um. Then, between the excipulum and the ascus, there is a layer of hyalines cells rounds of diameter 10-15um.
Asques unitunicates.

An idea?

Yannick
  • message #7194
Yannick Mourgues, 22-03-2009 21:06
Yannick Mourgues
Re:Patellaria
Excipulum in Indian ink...
  • message #7195
Yannick Mourgues, 22-03-2009 21:08
Yannick Mourgues
Re:Patellaria
Another picture of ascomes...
  • message #7196
Yannick Mourgues, 22-03-2009 21:08
Yannick Mourgues
Re:Patellaria
Young ones... with a brown hymenium.
  • message #7197
Hans-Otto Baral, 22-03-2009 21:26
Hans-Otto Baral
Re:Patellaria
Hi Yannick
The excipulum looks very much as in Durella. Apart from the angular apothecia I believe in Durella connivens. What guttules did you see in the paraphyses? The living paraph. contain pale yellow elongated vacuoles, I attach an image (section of margin).
  • message #7198
Hans-Otto Baral, 22-03-2009 21:28
Hans-Otto Baral
Re:Patellaria
Interesting is also in this species that the old spores inside collapsed asci stain pale blue in iodine.
  • message #7199
Hans-Otto Baral, 22-03-2009 21:30
Hans-Otto Baral
Re:Patellaria
Moreover, in a recent find there was an IKI-blue gel at the base of the apothecia. But this I saw the first time, maybe it is not constant.
  • message #7200
Yannick Mourgues, 22-03-2009 21:59
Yannick Mourgues
Re:Patellaria
Thank's Zotto.
Yes, paraphyses here contain pale yellow elongated vacuoles.
I've seen too blue spores in Lugol. Are they dead ? not mature ? I don't know why i haven't seen that at the first time.
But I haven't seen this IKI-blue gel at the base of apo.
Yannick
  • message #7203
Yannick Mourgues, 22-03-2009 21:59
Yannick Mourgues
Re:Patellaria
another pict...
  • message #7204
Hans-Otto Baral, 22-03-2009 22:10
Hans-Otto Baral
Re:Patellaria
Hi Yannick

wonderful! I do not understand this amyloid stain of old spores. Yes, they are always dead when they stain blue. But maybe they can also be submature, I really never understood that. Yours look like being aseptate, so they are not mature. D. connivens spores are ejected when 7-septate, and later they get much wider and constricted at the septa when they germinate.

I am unaware of a literature report on this quite unique reaction. I only remember that Strossmayeria is said to show it, while I never saw it there.

Zotto
Yannick Mourgues, 22-03-2009 22:22
Yannick Mourgues
Re:Patellaria
That's very interesting !
I haven't seen this blue-IKI reaction at first time !
Do you know where I could find a key for Durella genus ?
Thank's again.
Yannick
Hans-Otto Baral, 22-03-2009 22:35
Hans-Otto Baral
Re:Patellaria
There is little literature on Durella. I only remember my key (in the Helotiales-key). Dennis 1956 (British Helotiaceae) gives descriptions of some better known species.

Zotto
Yannick Mourgues, 22-03-2009 22:40
Yannick Mourgues
Re:Patellaria
OK. Thank's !
Yannick
Baeza Yajaira, 29-02-2012 19:11
Baeza  Yajaira
Re : Patellaria
hello Zotto

Where can i find your helotiales key? because i have some similiar apothecia in study that i think iis durella too ... 

hope you can help me.
Saludos :)
Stip Helleman, 01-03-2012 07:29
Stip Helleman
Re : Patellaria
Hi Yannick & Zotto,

My collection from Norway last year had this  bleuening at the base too
http://www.helotiales.nl/english/Species/Durella%20connivens.html
 
On the spores i did not observe than but looking at my pictures now there seems to be some bleuening inside the asci too


Cheers,
Stip
  • message #17592