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Mark Stevens"Hello everyone,I'm relatively new to microscopy (
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.. found by a seminar-participant, I do not know t
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Thomas Læssøehttps://svampe.databasen.org/observations/10572899
Pyrenopeziza/Mollisia on stems of Impatiens
Mlcoch Patrik,
06-11-2021 19:59
Hi,Please about help with determination of this collection.
Found on the dead stems of Impatiens glandulifera in the Czech Republic, 2. 9. 2021.
Marginal cells 16 x 5,5 um, excipular cells 13-15 x 10-12,5 um, dark brown. Asci IKI(+?), 35-40 X 5-7 um, paraphyses 30 x 1,8 um. Ascospores fusoid, hyaline, OCI=0-1, 7-9(9,5) x 1,7-2,5 um, Q=3,5, Me=8 x 2,3 um. KOH reaction of excipulum negative.
Any idea?? Thanks.
Hans-Otto Baral,
06-11-2021 20:53
Re : Pyrenopeziza/Mollisia on stems of Impatiens
To me this looks like a Mollisia. The crucial KOH reaction refers to the hymenium. Did you study living paraphyses? Herewith you can distinguish Pyrenopeziza which has no VBs (refractive vacuoles). To suggest a species name is difficult. M. revincta has a yellow KOH-reaction
Mlcoch Patrik,
06-11-2021 23:21
Re : Pyrenopeziza/Mollisia on stems of Impatiens
Thanks, this was microscopied in the freshes condition, what was possible.
Hans-Otto Baral,
07-11-2021 10:06
Re : Pyrenopeziza/Mollisia on stems of Impatiens
The your methods are not appropriate
- water as mountant
- don't apply pressure on the cover slip when studying sections and only very slight pressure for squash mounts.