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13-02-2020 00:11

Josep Torres Josep Torres

Hola.Imágenes tomadas el 2 de Febrero en la Repú

18-02-2020 22:32

Christine VALANCE

Bonjour, je sèche lamentablement sur cet asco tr

16-02-2020 18:00

Josep Torres Josep Torres

Hola.Especie fotografiada el 29 de Septiembre de 2

17-02-2020 22:31

Eric Bionne Eric Bionne

Bonjour à tous;Lasiosphaeria sur écorce de Fagus

17-02-2020 21:25

Enrique Rubio Enrique Rubio

Do you have some idea on this fungus with scutate

18-02-2020 08:53

Alain GARDIENNET Alain GARDIENNET

Hi forum, Have either of you ever observed the te

16-02-2020 16:48

Peter Thompson

Hello Everyone,I have found some pale yellowish re

17-02-2020 00:10

Valencia Lopez Francisco Javier

Hola a todos/asAdjunto algunas fotos de unos supue

17-02-2020 16:17

Björn Nordén

Hi!Can someone please tell me what this is? It has

16-02-2020 15:25

Riet van Oosten Riet van Oosten

Hello, Just a try :-) Found by Rene Hoogenboom,

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Sclerotinia sclerotiorum Spores adhesion
Ale Ale, 13-02-2020 16:34
Hi all,

I am working with SS spores on glass surfaces. My current problem is that they get stuck to the surface of glass very easily and they also tend to form clumps. I already tried surfactants such as Tween 20, Pluronic, PVA but I was not able to solve the problem. Any advice, tips or suggestions?.

Attached is a photo of stained spores. Thank you!
  • message #61493
David Malloch, 13-02-2020 17:56
David Malloch
Re : Sclerotinia sclerotiorum Spores adhesion
I have found that the spores of many white-spored agarics, especially Mycena, stick to glass slides in small clumps and are difficult to dislodge. Usually a drop of water will cause them to rehydrate and separate, but in some situations oil (mineral oil or even olive oil) seems to work. If you wish to make single-spore isolates, it would probably be better to discharge the spores on to an agar surface.
Ale Ale, 13-02-2020 19:13
Re : Sclerotinia sclerotiorum Spores adhesion
Hi David,

Thank you for your answer. I think I should explain a bit better my problem. Currently I have the spores flowing in a microchannel with DI water, so spores are already in contact with water. The flow is generated using a micropump. Some spores get stuck to the surface of this microchannel (glass) and wont move even at high flow rates. I noticed this problem not only in glass but in surfaces such as: Aluminum, Gold, PDMS, SU-8.

Spores get stuck easily and the surfactants I mentioned before did not help.
David Malloch, 13-02-2020 19:32
David Malloch
Re : Sclerotinia sclerotiorum Spores adhesion
This is beyond anything I have experienced. It would seem that the spores are genetically disposed to stick firmly to any surface they encounter; not a bad strategy for a plant parasite. Perhaps the "glue" is impervious to most normal surfactants or solvents. That sounds like a challenging and interesting problem.