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20-09-2020 22:01

Stephen Mifsud Stephen Mifsud

I have found Botryosporium longibrachiatum on dec

19-10-2016 20:32

Thorben Hülsewig

Hi there,last Sunday i found on rotten plant this

20-09-2020 15:39

Juuso Äikäs

These were also growing on deer dung. Spores 10.

20-09-2020 15:13

Juuso Äikäs

Some quite small ascos were growing on deer dung w

20-09-2020 18:11

Castillo Joseba Castillo Joseba

De esta mañana  sobre madera posiblemente de Fag

20-09-2020 15:02

Chris Yeates Chris Yeates

Bonjour tous A few days ago I collected a piece o

18-09-2020 22:38

Castillo Joseba Castillo Joseba

De esta mañana  en corteza de fagusHe visto unos

19-09-2020 11:04

Angel Pintos Angel Pintos

Hello,anybody has?Pseudoguignardia scirpi Gutner,

18-09-2020 22:49

Marek Capoun Marek Capoun

Hello everybody,I need help with the determination

01-03-2017 17:18

Enrique Rubio Enrique Rubio

Hi to everybodyCould you help me with a pdf copy o

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Sclerotinia sclerotiorum Spores adhesion
Ale Ale, 13-02-2020 16:34
Hi all,

I am working with SS spores on glass surfaces. My current problem is that they get stuck to the surface of glass very easily and they also tend to form clumps. I already tried surfactants such as Tween 20, Pluronic, PVA but I was not able to solve the problem. Any advice, tips or suggestions?.

Attached is a photo of stained spores. Thank you!
  • message #61493
David Malloch, 13-02-2020 17:56
David Malloch
Re : Sclerotinia sclerotiorum Spores adhesion
I have found that the spores of many white-spored agarics, especially Mycena, stick to glass slides in small clumps and are difficult to dislodge. Usually a drop of water will cause them to rehydrate and separate, but in some situations oil (mineral oil or even olive oil) seems to work. If you wish to make single-spore isolates, it would probably be better to discharge the spores on to an agar surface.
Ale Ale, 13-02-2020 19:13
Re : Sclerotinia sclerotiorum Spores adhesion
Hi David,

Thank you for your answer. I think I should explain a bit better my problem. Currently I have the spores flowing in a microchannel with DI water, so spores are already in contact with water. The flow is generated using a micropump. Some spores get stuck to the surface of this microchannel (glass) and wont move even at high flow rates. I noticed this problem not only in glass but in surfaces such as: Aluminum, Gold, PDMS, SU-8.

Spores get stuck easily and the surfactants I mentioned before did not help.
David Malloch, 13-02-2020 19:32
David Malloch
Re : Sclerotinia sclerotiorum Spores adhesion
This is beyond anything I have experienced. It would seem that the spores are genetically disposed to stick firmly to any surface they encounter; not a bad strategy for a plant parasite. Perhaps the "glue" is impervious to most normal surfactants or solvents. That sounds like a challenging and interesting problem.