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13-06-2025 09:41

Josep Torres Josep Torres

Hello.A cerebriform ascomycete sprouting scattered

13-06-2025 16:34

Andgelo Mombert Andgelo Mombert

Bonjour,Un petit discomycète qui me résiste. Il

24-07-2025 19:02

Castillo Joseba Castillo Joseba

De esta mañana en tronco caido de Haya A ver si

21-07-2025 19:22

Ethan Crenson

Hello all,  Here is an Orbilia found by a friend

14-07-2025 11:20

Michel Hairaud Michel Hairaud

Bonjour, Voici une espèce de  (?) Hyaloscyphace

18-07-2025 23:03

Josep Torres Josep Torres

Hello.Fruitings between 51 and 130 microns in tota

17-07-2025 11:55

Castillo Joseba Castillo Joseba

De ayer en bosque de hayas y abetos, en tieraEjemp

16-07-2025 17:34

Bernard Declercq Bernard Declercq

Hello,I have trouble distinguishing above mention

15-07-2025 13:27

Angel Pintos Angel Pintos

Hello, does anyone have access to the following ar

16-01-2023 21:31

Riet van Oosten Riet van Oosten

Hello, Nearby the find of Calycina claroflava on

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Iodophanus look-alike, but probably inoperculate
Edvin Johannesen, 03-07-2019 14:56
Edvin Johannesen
These tiny (0,5-1 mm) whitish to slightly pinkish apothecia were first thought to be an immature Iodophanus (aff. difformis), but after a thorough study in company with Prof. Trond Schumacher, it is concluded that it is most likely inoperculate, and possibly even partly with mature spores. It is believed to be bryophilous, since moss fragments have been observed attached to the apothecia. The spores are initially (in what is believed to be immature spores) multiguttulate (in water). Spores turn from more or less spherical to asymmetrically ellipsoid during maturation, then becoming slightly pointed at one end, and with one large guttule and a few smaller ones (in cotton blue). Spores seem to have one area void of guttules, thus being stained by CB. The possibly mature spores measure 17-19 x 10-13 um. Asci approx. 150 um long with a thickened apex. There is possibly a weak amyoid reaction (Meltzer) in the ascus wall, but no staining observed in the ascus tip. Paraphyse are slender and not expanded at the apex, thus "inoperculate"-like. Some clavate cells observed, staining violet in CB, but may be young asci. Some spores are seen to germinate at one or both ends. Some spores appear to be aborted within the asci.

Does anyone have a suggestion? Thanks in advance.
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Lothar Krieglsteiner, 03-07-2019 15:17
Lothar Krieglsteiner
Re : Iodophanus look-alike, but probably inoperculate

Hello Edvin,


perhaps something around Mniaecia nivea (and gloeocapsae) - the liverworts on the foto are characteristic.


I once found a collection in Norway, too :-) - it had larger spores.


I think the group is very insufficiently known.


Best regards, Lothar

Edvin Johannesen, 03-07-2019 15:32
Edvin Johannesen
Re : Iodophanus look-alike, but probably inoperculate
That was quick, Lothar - and what a great suggestion! You must obviously be on the right track. I assume it is your 2015 collection from Dovrefjell you are referring to:


Actually this one was also collected in the Dovrefjell area, a bit further south (Grimsdalen), in the subalpine region.

We have taken a sample for DNA sequencing (Schumacher will take care of that). Perhaps Zotto has an opinion?

Thank you so much. I wish I had the necessary literature in order to arrive at the right species, if possible. Can you help?

Best regards,

Edvin
Hans-Otto Baral, 03-07-2019 17:08
Hans-Otto Baral
Re : Iodophanus look-alike, but probably inoperculate
Hi Edvin & Lothar

do you have more photos in water? In CB I would not describe the oil drops, you get artifacts there. One large drop is surely due to coalescence provoked by lactophenol.

A weakly amyloid reaction is possible, do you have Lugol as well? I remember Trizodia has such reaction and something related to it, whereas Mniaecia is inamyloid.

Maturity you can only estimate in water, then you see which asci are alive and mature.

Zotto
Edvin Johannesen, 04-07-2019 10:40
Edvin Johannesen
Re : Iodophanus look-alike, but probably inoperculate
Hi Zotto (and Lothar)!

I am adding the remaining photos I have in water, not sure it helps much, but some definitely show immature spores/asci. Sorry about the phase contrast at 1000x oil, I just use it sometimes to be able to see hyaline hyphae.

I do have Lugol and Barals Lösung. I decided to dry the material yesteday, after keeping it alive for two weeks, in and out of the fridge, hoping that it would mature (which it apparently didn't). I believe I did already try Lugol, without seeing any reaction. Schumacher claimed to see a weak wall reaction with Meltzer (but I was not convinced :-)

Eddi
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Hans-Otto Baral, 04-07-2019 11:31
Hans-Otto Baral
Re : Iodophanus look-alike, but probably inoperculate
Good, so you see that the living spores are multiguttulate, without large LBs. They look mature in my opinion, only the asci are dead. 

Apparently the species has inamyloid asci, when you did not get a reaction.

For Mniaecia nivea and M. gloeocapsa I see only LSU in GenBank, no ITS. Also Trizodia includes LSU, so when trying a sequence, LSU would be important.
Edvin Johannesen, 04-07-2019 12:05
Edvin Johannesen
Re : Iodophanus look-alike, but probably inoperculate
I have now re-wetted and sacrificed one more (immature) apothecium and mounted in Lugol. I am uncertain whether there is a weak reaction or not in the ascus wall (three first images). What do you think? The three last images are in water, showing immature spores.
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Edvin Johannesen, 04-07-2019 12:05
Edvin Johannesen
Re : Iodophanus look-alike, but probably inoperculate
And thanks for the tip on LSU!
Lothar Krieglsteiner, 04-07-2019 12:44
Lothar Krieglsteiner
Re : Iodophanus look-alike, but probably inoperculate

Hi Edvin,


for me this looks negative :-)


Best regards, Lothar

Edvin Johannesen, 04-07-2019 14:06
Edvin Johannesen
Re : Iodophanus look-alike, but probably inoperculate
Zotto,

It is strange that you find only LSU for Mniaecia in Genbank, since sequences for all three regions (ITS/LSU/SSU) are cited in this paper from 2018 (Fig. 2):


and in this 2019 paper (supplementaryTable 1):


I don't have an explanation for this.

Interestingly, Trizodia branches out as a separate lineage in the first paper.

Regards,

Edvin