tiny discomycete on Glyceria - Forum ASCOFrance

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tiny discomycete on Glyceria

Chris Yeates, 18-06-2019 16:55

Bonjour tous

a recent collection of damp leaves of Glyceria maxima has numerous, generally rather scattered, pale sessile ascomata.

The margins are "fringed" rather than hairy, and under the microscope can be seen as projecting marginal cells, a considerable number of which are swollen up to 4.6µm and contain vacuolar material (hyaline >possibly very pale straw colour).

Asci 30.3-39 x 4.9-5.9µm; "Psilachnum guttules" not seen; although difficult to see I think there were croziers (see image); apex blue in Lugol's.

Ascospores mostly staight, narrowly clavate, with a few small mostly polar guttules, 7.2-8.7 x 1.9-2.3µm.

I think this is Psilachnum eburneum (Pezizella eburnea). The absence of a guttule in the asci may well be due to stage of development. Dennis in CMI Mycological Paper 62 (1956) makes no mention of the vacuolar content at the hair apices, but I suspect this was due to him working from dried material (this v. content disppeared when Lugol was added to the mount). An image of Stip's seems to show similar content in the apical cell: http://www.helotiales.nl/english/Species/Psilachnum_eburneum.htm

Any comments welcome

Merci

Chris