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28-07-2011 18:31

Alex Akulov Alex Akulov

Dear FriendsToday I made the pdf file of Velenovsk

26-03-2024 17:14

Juuso Äikäs

These little black ascos were growing on a dead, d

27-03-2024 22:53

Charles Aron Charles Aron

Hi All,Recently I came across these minute, setose

25-03-2024 13:41

B Shelbourne B Shelbourne

• Hyaloscyphaceae (no VBs), Hyaloscypha: Macro a

25-03-2024 21:27

Riet van Oosten Riet van Oosten

Hello, Found by Laurens van der Linde, March 2024

26-03-2024 14:37

DAVID MARCOS DAVID MARCOS

Spores (telles qu'elles apparaissent à l'intérie

24-03-2024 08:27

Thierry Blondelle Thierry Blondelle

HiOn Hedera helix fallen branchEcological habitat:

26-03-2024 11:06

michel bertrand

Bonjour, Malgré de nombreuses recherches, je n'a

25-03-2024 14:08

Ursula Kozik

Bonjour et bonne journée, On m'a envoyé une tro

25-03-2024 03:56

B Shelbourne B Shelbourne

• Scuttelinia: Macro and habitat.• S. scutella

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greenish 1-septate spores, saccate asci
Ethan Crenson, 11-06-2019 00:08
Hello all,

I found a few of these tiny black cushions on a branch last weekend in a cemetery in New York.  They grew on the same branch as the Orbilia and Barbatosphaeria barbirostris which I posted about earlier.  They are smaller than 1mm diameter, sessile, gelatinous in texture. The asci are saccate.  The spores 1-septate, greenish in color and measure 16-21 x 8-10µm.  In the photos you will see that they appear to be growing in a white subiculum, but I am not certain that it relates to these apothecia.  Any ideas?

Thank you in advance.

Ethan
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Hans-Otto Baral, 11-06-2019 07:39
Hans-Otto Baral
Re : greenish 1-septate spores, saccate asci
You need to apply diluted Lugol to see the iodine reaction (possibly lateral wall red, apical wall a thick blue cap, then it is a Dactylospora, now called Sclerococcum.
Ethan Crenson, 11-06-2019 18:46
Re : greenish 1-septate spores, saccate asci
Zotto,

Thank you.  When you said diluted Lugol's I didn't realize that it had to be very diluted.  My first three mounts were rubbish.  This series of images shows the section mounted in extremely dilute Lugol's to which I gradually added a less dilute solution.  I think I see the faint reddish tint on the ascus wall (indicated with an arrow).  The blue reaction at the apex of the ascus is hard to miss.

Ethan
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Ethan Crenson, 11-06-2019 18:47
Re : greenish 1-septate spores, saccate asci
Here's the picture a little closer...
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Hans-Otto Baral, 11-06-2019 20:21
Hans-Otto Baral
Re : greenish 1-septate spores, saccate asci
Indeed, this is a Lecanoromycete and in all lichens the reaction is very strong. But it is possible by strong squashing to see the details.
Non-lichenicolous species are difficult to identify. Are you sure with the big spore size? Usually spores are smaller.
Ethan Crenson, 11-06-2019 22:37
Re : greenish 1-septate spores, saccate asci
In fact I did read the wrong notes when I transcribed the spore size. My apologies. Spores measure: 9-11 x 4-5µm.