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17-10-2018 07:10

Ethan Crenson

In Northeastern North America.  On the banks of a

15-10-2018 18:55

hannie wijers

Hello, I'd like some help if possibleIn Beilen I

16-10-2018 23:33

Valencia Lopez Francisco Javier

Hola a todos/asPeziza recolectada en el sur de Esp

03-10-2018 22:13

François Bartholomeeusen

Good evening forum members,Can this be Hyalorbilia

14-10-2018 19:25

Marja Pennanen

Hi, on a Populus leaf among other things, I found

15-10-2018 10:52

Castillo Joseba Castillo Joseba

Del pasado sabado en bosque de avellanos,   pare

14-10-2018 22:24

Castillo Joseba Castillo Joseba

De ayer sobre tierra en terrenos de cultivo con av

10-10-2018 23:11

Viktorie Halasu Viktorie Halasu

Hello forum,I would like to ask how to call correc

12-10-2018 20:15

Thorben Hülsewig

Hi there,two week ago i found this asco on pine co

12-10-2018 05:29

Danny Newman Danny Newman

Substrate/Host: on hollow, herbaceous twig in a ro

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Small, aggregated Encoelia
Edvin Johannesen, 08-10-2018 14:51
Edvin JohannesenI came across these dense mats of apothecia seated on a dark, but whitish hoary subiculum, growing on the bark of a huge, fallen deciduous tree (most likely Salix caprea or Popolus tremulae), in a nature reserve i Southern Norway. I guess this is an Encoelia, but it doesn't seem fit any of the species known from Norway. It comes closest to E. pruinosa. However, the apothecia are only 1-2 mm in diam. and the paraphyses ar not noticeably widened at the tips.  The spores are curved, 11x3 microns, apprarently with some sort of thickening or body at each end, becoming evident during focussing. The attached micrographs are taken at 1000x oil immersion, phase contrast, in water. I have not yet used other media. Does anyone know this species?
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Hans-Otto Baral, 08-10-2018 15:34
Hans-Otto Baral
Re : Small, aggregated Encoelia
The fungus looks pretty good, but with the micros I have no clear idea.
Indeed, Encoelia pruinosa )or now Sclerencoelia pruinosa) would also be my first option.But it should occur on living trunks. There should be lots of crystals outside, which is probable because of the pruinose surface.

Spore size could be larger, but apo size seems to be variable.

To clarify whether it is Salix or Populus needs to look at a radial or tangential section for homo/heterocellular radial rays.
Zotto
Lothar Krieglsteiner, 08-10-2018 15:36
Lothar Krieglsteiner
Re : Small, aggregated Encoelia

Hello Edvin,


very interesting fungus.


The photos are with phase contrast as you write. Why? I do not use it at all and would much prefer to see "normal" photos. I think I am not alonè with this (?).


Best regards, Lothar

Edvin Johannesen, 08-10-2018 16:30
Edvin Johannesen
Re : Small, aggregated Encoelia
Thanks for the comments. I know you guys don't fancy phase contrast :). What mounting medium do you recommend for obtaining optimal contrast for photomicrography, when spores are hyaline? I have lugol, cotton blue, Congo red, and Baral's medium, apart from KOH. I am not working with ascomycetes, primarily, so grateful for advice on mounting media.
Edvin Johannesen, 08-10-2018 16:32
Edvin Johannesen
Re : Small, aggregated Encoelia
Unfortunately I do no have a section of the wood. Parts of the tree (the branch) could still be alive.
Hans-Otto Baral, 08-10-2018 17:05
Hans-Otto Baral
Re : Small, aggregated Encoelia
If you have a portion with apothecia, then there is usually enough wood to examine.

There is enough contrast when mounting in water, especially when cells are alive. Look recent contribution by Elisabeth.

What you mean with polar thickenings of the spores, I assume you mean the light polar region seen especially in one spore. I am unable to say in photos with phasecontrast, but I could see clearer without that device, maybe polar oil drops.
Edvin Johannesen, 08-10-2018 21:35
Edvin Johannesen
Re : Small, aggregated Encoelia
I think you are right about the polar oil drops (see photos below). Correct, there is also a small bit of wood, which I will try to identify to Salix vs. Populus.  Here are a few spore photos in water, plain transmitted light. I know - these are shitty pictures, but this is the best I could do (shot directly through the eyepiece). Does that help?
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Hans-Otto Baral, 08-10-2018 22:35
Hans-Otto Baral
Re : Small, aggregated Encoelia
This looks better :-)

I don't understand the grainy background but otherwise the photos are splendid.

So from the scale I get 11.5-13.5 x 2.6-3.3 µm which would fit well with other collections.
Edvin Johannesen, 08-10-2018 23:21
Edvin Johannesen
Re : Small, aggregated Encoelia
Thanks! I guess the grainy background is dust somewhere in the optical path. I need to do some cleanup ...  You mean it fits well with Sclerencoelia pruinosa?
Hans-Otto Baral, 08-10-2018 23:24
Hans-Otto Baral
Re : Small, aggregated Encoelia
Judging from the spores alone - yes.