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17-11-2009 22:22

Pablo Chacón Pablo Chacón

Bonne nuit, Voir si vous m'avez élaguée appor

07-12-2015 14:17

Zugna Marino Zugna Marino

Buon giorno a tutti, ad un primo momento, non ess

25-11-2012 20:32

Bometon Javier Bometon Javier

Ascomas cupoliformes abiertos lateralmente, himeni

25-01-2026 16:08

Malcolm  Greaves Malcolm Greaves

This Geoglossum had spores mostly 70-80 (87) with

27-01-2026 11:43

Malcolm  Greaves Malcolm Greaves

Is anyone with experience of DNA testing able to t

26-01-2026 11:49

Margot en Geert Vullings

We found this possible anamorph on a dead Cytisus

25-01-2026 23:23

Tomaz Vucko Tomaz Vucko

Hello! I found this species that resembles Delitsc

18-01-2026 12:24

Josep Torres Josep Torres

Hello.An anamorph located on the surface of a thin

23-01-2026 21:50

Cameron DK

I am looking for this please publication. is anyon

10-01-2026 20:00

Tom Schrier

Hi all,We found picnidia on Protoparmeliopsis mur

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Lophodermium on pine needles
Lepista Zacarias, 21-03-2017 18:46
Hi everyone,
This fungus was found on pine needles about two weeks ago. I don't know the exact species of pine, but the needles are double and do not excceed 10 cm, some with half of that size.
Ascomata black with less than 1mm long, slightly raised and aligned lengthwise on the needle having a slit with greyish labia;
Asci 100-115 x 11-13 µm;
Ascospores hyaline, acicular almost as long as the asci.
Is it possible to identify the species?
Thanks in advance for you help,
zaca
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Joanne Taylor, 21-03-2017 19:21
Re : Lophodermium on pine needles
Hi there,

it looks like your needles are trash needles (those which have died prematurely as the branch has been broken) and not senesced litter needles (died of old age). If they are the former and if your host is Scots Pine, then it might either be L. conigenum or L. seditiosum. You then need to look at your sections and look for displaced epidermal cells (the ascomata are more or less sub epidermal but a few cells get left at the base of the ascomata depending on the species). If thereare none then it is L.seditiosum and if there are a few (less than 7)scattered about then it will be L. conigenum. This information is from David Minter's amazing studies on Lophodermium!
Cheers,
Jo
Lepista Zacarias, 21-03-2017 20:07
Re : Lophodermium on pine needles
Hi Joanne,
Thanks for your message.
I'm afraid to not understood completely the meaning of the displaced cells as you call them. Thus, I upload the (bad) section I took from microscopy. Where in the photo I should look for those cells? 
Best Regards,
zaca
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Joey JTan, 21-03-2017 20:48
Re : Lophodermium on pine needles
I can't make them out in your picture, it usually requires a pretty thin vertical section.  Sectioning air-dried specimens with a safety razor blade is often easier than sectioning fresh specimens.

Minter describes and illustrates the concept of displaced epidermal cells in:

Minter, D. W., Staley, J. M., & Millar, C. S. (1978). Four species of Lophodermium on Pinus sylvestris. Transactions of the British Mycological Society, 71(2), 295-301.

Minter, D.W., 1981. Lophodermium on pines. Commonwealth Mycological Institute.

It relates to how the ascoma is embedded in the host tissue.  It took me a bit of time to learn how to readily identify this character; collecting and comparing different subcuticular and subepidermal Lophodermium species helped.

Of course there are cryptic species complexes in Lophodermium, which makes identification somewhat tenuous for some species...
Lepista Zacarias, 21-03-2017 21:31
Re : Lophodermium on pine needles
Thanks, Joey,
for your comments and references.
I will try to get some literature on the genus and, maybe make a better section.
Best regards,
zaca
Lepista Zacarias, 22-03-2017 00:36
Re : Lophodermium on pine needles
Hi again,
The needles are ver britle, making difficult to make good sections. I attached my best result, from where I could not see any "displaced cells" from the ascoma left on the substrate.
Cordialment,
zaca
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Peter Püwert, 22-03-2017 02:53
Peter Püwert
Re : Lophodermium on pine needles
Hi Zaca,
this is not often simple to determine this species.
I have sent some literature.
Greetings Peter.
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