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25-09-2024 22:18

Bometon Javier Bometon Javier

Apotecios 100 y 350 um, estipitados.Pararafisis la

25-09-2024 14:24

B Shelbourne B Shelbourne

• Macro and habitat suggest Rutstroemia, and pos

26-09-2024 17:25

Hans-Otto Baral Hans-Otto Baral

Does someone have a pdf of this paper? I have it

23-09-2024 17:24

Karen Poulsen

Hi there, I found a few very small apothecia on o

24-09-2024 18:27

Pierre-Yves Julien

Récolte le 01/09/2024 – Paris (75) – France â

25-09-2024 20:07

François Bartholomeeusen

After I dipped a fallen Ilex leaf in water for a d

22-09-2024 20:24

Robin Isaksson Robin Isaksson

Dear all, I did found this one on on S. acauli

23-09-2024 20:46

B Shelbourne B Shelbourne

• Macro and habitat suggest Gelatinodiscaeae.•

24-09-2024 11:35

Peter Welt Peter Welt

Search Looking for an item again. Who can help? T

06-07-2020 05:51

Yulia Lytvynenko Yulia Lytvynenko

Dear friends.Looking for a copy of the following w

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Peziza blueing
Malcolm Greaves, 17-06-2016 00:04
Malcolm  GreavesCan anyone tell me if it is normal for the blueing of the ascus tip to only last a very short time. I have what I think is Peziza vesiculosa from soil in the garden and the blue only lasted a few minutes. These were taken only a few minutes apart
Thanks
Mal
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  • message #43371
  • message #43371
Hans-Otto Baral, 17-06-2016 07:24
Hans-Otto Baral
Re : Peziza blueing
I am not sure why your photo has such a red tone. Which reagents did you apply, Melzer or Lugol? It is necessary to see the yellow colour of iodine in order to say something. For instance if you add KOH, the reaction logically disappears.
Zotto
Malcolm Greaves, 17-06-2016 10:33
Malcolm  Greaves
Re : Peziza blueing
Hi Zotto
It was a bit of poor setup of the camera software. I will try and get some more representative photos today. Both the photos were in Lugol.
Mal
Now attached. Not perfect but much more representative.
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Hans-Otto Baral, 17-06-2016 10:52
Hans-Otto Baral
Re : Peziza blueing
The phenomenon exists that higher concentrations of Lugol suppress the blue reaction. this is some kind of hemiamyloid, which is in fact a change to a dirty rid-brown. I am not sure if this is the case here. You can easily test this by adding more Lugol so that the ascoplasm gets distinctly stained.

You can also test KOH-pretreatment. After washing with water you can repeat the test, the effect should then be absent.
Malcolm Greaves, 17-06-2016 10:55
Malcolm  Greaves
Re : Peziza blueing
Didn't seem to fade with Meltzers.
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  • message #43376