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This tiny, rather "rough" erumpent asco was found

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Philippe PELLICIERBonjour, pensez-vous que S. ceijpii soit le nom co
Little pinkish asco
Zuzana Sochorová (Egertová),
11-05-2016 20:37

this very little ascomycete grew on soil in a margin of a way, among protonemata, on sandstone background, Czechia.
Apothecia about 0.2 mm broad, sessile, pinkish, rounded, without hairs, convex.
Asci cylindric-clavate, non-amyloid, with 8 biseriately arranged spores in the upper part.
Spores 11-12.5 x 5.5-6 micrometers (unfortunately the collection contains mostly young apothecia, so I couldn´t observe many mature spores), smooth, hyaline, filled with many little droplets. In young spores (inside asci) there is a well visible a septum (or rarely two).
Paraphyses 1-1.5 micrometers wide, bent or curved, septate, hyaline.
Excipulum with subglobose to subangular cells, 7-13.5 micrometers broad.
It resembles me the genus Mniaecia.
Zuzana
François Valade,
11-05-2016 21:03

Re : Little pinkish asco
Hello
It reminds me Discinella lividopurpurea. Let's wait for Zotto's point of view.
François
It reminds me Discinella lividopurpurea. Let's wait for Zotto's point of view.
François
Hans-Otto Baral,
11-05-2016 21:36

Re : Little pinkish asco
I imagine that this might be a Mniaecia! Incredible, but it looks a bit so. Discinella limosella/lividopurpurea has more elongate spores with a rather low lipid content, amyloid asci, and straight paraphyses with VBs. The septate spores are surely overmature (such are not rarely found inside dead asci!), the mature guttulate spores seem to be aseptate. Do you have a photo of the ascus apex in dead state? Is it thick-walled?
Such strange collections would benefit from a DNA analysis.
Such strange collections would benefit from a DNA analysis.
Zuzana Sochorová (Egertová),
11-05-2016 22:27

Re : Little pinkish asco
Thank you, Francois and Zotto.
I made photos of asci in cotton blue and KOH, but they are of a very poor quality.
I will let them grow few more days in a fridge and then sample few apothecia for sequencing (although I don´t know whether it will be succesful in such little fungi).
I made photos of asci in cotton blue and KOH, but they are of a very poor quality.
I will let them grow few more days in a fridge and then sample few apothecia for sequencing (although I don´t know whether it will be succesful in such little fungi).
Hans-Otto Baral,
11-05-2016 22:32

Re : Little pinkish asco
in opened asci the thickening is not so good to see, but anyhow I see there is one.