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Asco blanc, Hymenoscyphus ???
Patrice TANCHAUD,
22-10-2015 16:15
récolte faite en tourbière avec sphaignes, apothécies sur de petites tiges pourrissantes. Apothécies cupuliformes, non stipitées. Asques d'une moyenne de 105 µm, très faiblement amyloïdes. Spores 11-16 x 3,5-5 µm.
Merci d'avance pour vos suggestions.
Harvest made with sphagnum bog, apothecia on small rotting stems. Apothecia cup-shaped, without stipe. Asci 105 µm, très faiblement amyloïdes. Spores 11-16 x 3,5-5 µm.
Thank you in advance our suggestions.
Patrice
Doc1-0015.pdf
Hans-Otto Baral,
22-10-2015 16:50
Re : Asco blanc, Hymenoscyphus ???
Hi Patrice
is it actually MLZ what you used? This could be a Pezoloma, and a hemiamyloid reaction of the apical ring would be possible.
The externe layer could be the gelatinized outer layer typical of the genus.
Zotto
is it actually MLZ what you used? This could be a Pezoloma, and a hemiamyloid reaction of the apical ring would be possible.
The externe layer could be the gelatinized outer layer typical of the genus.
Zotto
Patrice TANCHAUD,
22-10-2015 17:13
Re : Asco blanc, Hymenoscyphus ???
Melzer 2015.
Nothing with Lugol.
Nothing with Lugol.
Hans-Otto Baral,
22-10-2015 17:20
Re : Asco blanc, Hymenoscyphus ???
Does this mean you used MLZ bought in 2015? And als only very pale blue reaction in Lugol?
If you don't have Lugol, try KOH-pretreatment whether the reaction gets more intense.
If you don't have Lugol, try KOH-pretreatment whether the reaction gets more intense.
Patrice TANCHAUD,
22-10-2015 17:42
Re : Asco blanc, Hymenoscyphus ???
Sorry, I specify :
- MLZ bought in 2015, yes, and very very pale blue reaction, or no reaction.
- I have Lugol bought in 2014, and the reaction is negative.
Patrice
- MLZ bought in 2015, yes, and very very pale blue reaction, or no reaction.
- I have Lugol bought in 2014, and the reaction is negative.
Patrice
Hans-Otto Baral,
22-10-2015 17:57
Re : Asco blanc, Hymenoscyphus ???
This is unusal that MLZ gives a more distinct reaction. I see a pale blue on your pics. If your Lugol is still dark brown (it should so), and if you still have material, it would be helpful to see a mount in IKI.
Patrice TANCHAUD,
22-10-2015 18:49
Re : Asco blanc, Hymenoscyphus ???
I have no IKI, sorry.
I also tried :
- KOH then melzer : nothing better
- KOH then lugol : no reaction
My lugol is color amber.
I also tried :
- KOH then melzer : nothing better
- KOH then lugol : no reaction
My lugol is color amber.
Hans-Otto Baral,
22-10-2015 18:58
Re : Asco blanc, Hymenoscyphus ???
Lugol = IKI, but the concentration is possibly too week when it is amber in the bottle. Look my homepage, last photo
http://www.invivoveritas.de/iodine-reaction/
anyway it shoudl give a reaction also when yellow-amber.
http://www.invivoveritas.de/iodine-reaction/
anyway it shoudl give a reaction also when yellow-amber.
Patrice TANCHAUD,
22-10-2015 20:04
Re : Asco blanc, Hymenoscyphus ???
No doubt, my lugol is too clear, it is surprising because it is not old.
Hans-Otto Baral,
22-10-2015 20:33
Re : Asco blanc, Hymenoscyphus ???
If you bozught it as Lugo it has usually about 0.3% iodine. This is the standard and useful for lichens, although I prefer also there a higher concentration because some blue reactions turn red only then.
Patrice TANCHAUD,
22-10-2015 20:42
Re : Asco blanc, Hymenoscyphus ???
Thanks for the information.
François Valade,
27-10-2015 10:23
Re : Asco blanc, Hymenoscyphus ???
Hello
Concentration of the Lugol provided to Patrice is 1 % iodine, 2% KI pour 100 ml.
I have now requested that KI raises to 3% for the Lugol reactive
Is it convenient for you?
François
Concentration of the Lugol provided to Patrice is 1 % iodine, 2% KI pour 100 ml.
I have now requested that KI raises to 3% for the Lugol reactive
Is it convenient for you?
François
Hans-Otto Baral,
27-10-2015 10:57
Re : Asco blanc, Hymenoscyphus ???
Hi Francois
I put the KI and crystals without using any weighing machine, so I cannot say for sure that my concentration is 1%. When I wrote my article in 1987 I measured it exactly, and there noticed the decrease in concentration during some months if you keep it in small bottles with a plastic lid.
Maybe I presently use a better lid, it seems not to permit the iodine to escape. When did Patrice get the solution?
3% maybe o.k., but it is then more difficult to observe the blue part of the RB reaction and you easily get the preparation overstained.
I put the KI and crystals without using any weighing machine, so I cannot say for sure that my concentration is 1%. When I wrote my article in 1987 I measured it exactly, and there noticed the decrease in concentration during some months if you keep it in small bottles with a plastic lid.
Maybe I presently use a better lid, it seems not to permit the iodine to escape. When did Patrice get the solution?
3% maybe o.k., but it is then more difficult to observe the blue part of the RB reaction and you easily get the preparation overstained.